Zinc status and age-related changes in peripheral blood leukocyte subpopulations in healthy men and women aged 55-70y: the ZENITH study

CF Hodkinson, M Kelly, C Coudray, WS Gilmore, BM Hannigan, JM O'Connor, JJ Strain, Julie Wallace

Research output: Contribution to journalArticle

Abstract

Objective: To determine zinc status and age-related changes in the immune function of healthy late-middle-aged men and women (aged 55-70 y). Design: Observational study. Setting: Population of Northern Ireland. Subjects: Apparently healthy, free-living individuals (45 men, 48 women) aged 55-70 y. Intervention: Zinc status markers were analysed by flame atomic absorption spectrometry and commercially available kits. Immune function was assessed by flow cytometry. Results: Serum and erythrocyte zinc concentrations were 13.0 (s.d. 1.40) mu mol/l and 222 (s.d. 48.2) mu mol/l, respectively. Serum alkaline phosphatase (ALP) concentrations were 76.8 (s.d. 16.1) U/I; women showed significantly higher concentrations of ALP (P=0.011). Women demonstrated (1) a significant inverse correlation in naive T lymphocytes, specifically naive T-helper lymphocytes (% expression, r= -0.364, P=0.007 and absolute count, r= -0.275, P=0.036) with age and (2) a significant positive correlation between late activation of T lymphocytes (% expression, r=0.299, P=0.019 and absolute count, r=0.260, P=0.039) with advancing age. Men demonstrated a significant positive correlation in the % expression of (CD3-/CD16 + CD56 +) natural killer (NK) cells with age (r=0.316, P=0.017). Conclusions: Between the ages of 55 and 70 y, healthy individuals experience significant alterations in immune function; however, such changes appear largely sex specific. Given the reported importance of adequate zinc status in maintaining optimal immune function, further studies are required to explore the effect of enhanced zinc status on emerging immune deficiencies in cell-mediated immunity in healthy 55-70 y olds.
LanguageEnglish
PagesS63-S67
JournalEuropean Journal of Clinical Nutrition
Volume59
Issue numberSuppl.
DOIs
Publication statusPublished - Nov 2005

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Zinc
Leukocytes
Alkaline Phosphatase
T-Lymphocytes
Northern Ireland
Helper-Inducer T-Lymphocytes
Serum
Cellular Immunity
Natural Killer Cells
Observational Studies
Spectrum Analysis
Healthy Volunteers
Flow Cytometry
Erythrocytes
Population

Cite this

Hodkinson, CF ; Kelly, M ; Coudray, C ; Gilmore, WS ; Hannigan, BM ; O'Connor, JM ; Strain, JJ ; Wallace, Julie. / Zinc status and age-related changes in peripheral blood leukocyte subpopulations in healthy men and women aged 55-70y: the ZENITH study. In: European Journal of Clinical Nutrition. 2005 ; Vol. 59, No. Suppl. pp. S63-S67.
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title = "Zinc status and age-related changes in peripheral blood leukocyte subpopulations in healthy men and women aged 55-70y: the ZENITH study",
abstract = "Objective: To determine zinc status and age-related changes in the immune function of healthy late-middle-aged men and women (aged 55-70 y). Design: Observational study. Setting: Population of Northern Ireland. Subjects: Apparently healthy, free-living individuals (45 men, 48 women) aged 55-70 y. Intervention: Zinc status markers were analysed by flame atomic absorption spectrometry and commercially available kits. Immune function was assessed by flow cytometry. Results: Serum and erythrocyte zinc concentrations were 13.0 (s.d. 1.40) mu mol/l and 222 (s.d. 48.2) mu mol/l, respectively. Serum alkaline phosphatase (ALP) concentrations were 76.8 (s.d. 16.1) U/I; women showed significantly higher concentrations of ALP (P=0.011). Women demonstrated (1) a significant inverse correlation in naive T lymphocytes, specifically naive T-helper lymphocytes ({\%} expression, r= -0.364, P=0.007 and absolute count, r= -0.275, P=0.036) with age and (2) a significant positive correlation between late activation of T lymphocytes ({\%} expression, r=0.299, P=0.019 and absolute count, r=0.260, P=0.039) with advancing age. Men demonstrated a significant positive correlation in the {\%} expression of (CD3-/CD16 + CD56 +) natural killer (NK) cells with age (r=0.316, P=0.017). Conclusions: Between the ages of 55 and 70 y, healthy individuals experience significant alterations in immune function; however, such changes appear largely sex specific. Given the reported importance of adequate zinc status in maintaining optimal immune function, further studies are required to explore the effect of enhanced zinc status on emerging immune deficiencies in cell-mediated immunity in healthy 55-70 y olds.",
author = "CF Hodkinson and M Kelly and C Coudray and WS Gilmore and BM Hannigan and JM O'Connor and JJ Strain and Julie Wallace",
year = "2005",
month = "11",
doi = "10.1038/sj.ejcn.1602301",
language = "English",
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pages = "S63--S67",
journal = "European Journal of Clinical Nutrition",
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Zinc status and age-related changes in peripheral blood leukocyte subpopulations in healthy men and women aged 55-70y: the ZENITH study. / Hodkinson, CF; Kelly, M; Coudray, C; Gilmore, WS; Hannigan, BM; O'Connor, JM; Strain, JJ; Wallace, Julie.

In: European Journal of Clinical Nutrition, Vol. 59, No. Suppl., 11.2005, p. S63-S67.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Zinc status and age-related changes in peripheral blood leukocyte subpopulations in healthy men and women aged 55-70y: the ZENITH study

AU - Hodkinson, CF

AU - Kelly, M

AU - Coudray, C

AU - Gilmore, WS

AU - Hannigan, BM

AU - O'Connor, JM

AU - Strain, JJ

AU - Wallace, Julie

PY - 2005/11

Y1 - 2005/11

N2 - Objective: To determine zinc status and age-related changes in the immune function of healthy late-middle-aged men and women (aged 55-70 y). Design: Observational study. Setting: Population of Northern Ireland. Subjects: Apparently healthy, free-living individuals (45 men, 48 women) aged 55-70 y. Intervention: Zinc status markers were analysed by flame atomic absorption spectrometry and commercially available kits. Immune function was assessed by flow cytometry. Results: Serum and erythrocyte zinc concentrations were 13.0 (s.d. 1.40) mu mol/l and 222 (s.d. 48.2) mu mol/l, respectively. Serum alkaline phosphatase (ALP) concentrations were 76.8 (s.d. 16.1) U/I; women showed significantly higher concentrations of ALP (P=0.011). Women demonstrated (1) a significant inverse correlation in naive T lymphocytes, specifically naive T-helper lymphocytes (% expression, r= -0.364, P=0.007 and absolute count, r= -0.275, P=0.036) with age and (2) a significant positive correlation between late activation of T lymphocytes (% expression, r=0.299, P=0.019 and absolute count, r=0.260, P=0.039) with advancing age. Men demonstrated a significant positive correlation in the % expression of (CD3-/CD16 + CD56 +) natural killer (NK) cells with age (r=0.316, P=0.017). Conclusions: Between the ages of 55 and 70 y, healthy individuals experience significant alterations in immune function; however, such changes appear largely sex specific. Given the reported importance of adequate zinc status in maintaining optimal immune function, further studies are required to explore the effect of enhanced zinc status on emerging immune deficiencies in cell-mediated immunity in healthy 55-70 y olds.

AB - Objective: To determine zinc status and age-related changes in the immune function of healthy late-middle-aged men and women (aged 55-70 y). Design: Observational study. Setting: Population of Northern Ireland. Subjects: Apparently healthy, free-living individuals (45 men, 48 women) aged 55-70 y. Intervention: Zinc status markers were analysed by flame atomic absorption spectrometry and commercially available kits. Immune function was assessed by flow cytometry. Results: Serum and erythrocyte zinc concentrations were 13.0 (s.d. 1.40) mu mol/l and 222 (s.d. 48.2) mu mol/l, respectively. Serum alkaline phosphatase (ALP) concentrations were 76.8 (s.d. 16.1) U/I; women showed significantly higher concentrations of ALP (P=0.011). Women demonstrated (1) a significant inverse correlation in naive T lymphocytes, specifically naive T-helper lymphocytes (% expression, r= -0.364, P=0.007 and absolute count, r= -0.275, P=0.036) with age and (2) a significant positive correlation between late activation of T lymphocytes (% expression, r=0.299, P=0.019 and absolute count, r=0.260, P=0.039) with advancing age. Men demonstrated a significant positive correlation in the % expression of (CD3-/CD16 + CD56 +) natural killer (NK) cells with age (r=0.316, P=0.017). Conclusions: Between the ages of 55 and 70 y, healthy individuals experience significant alterations in immune function; however, such changes appear largely sex specific. Given the reported importance of adequate zinc status in maintaining optimal immune function, further studies are required to explore the effect of enhanced zinc status on emerging immune deficiencies in cell-mediated immunity in healthy 55-70 y olds.

U2 - 10.1038/sj.ejcn.1602301

DO - 10.1038/sj.ejcn.1602301

M3 - Article

VL - 59

SP - S63-S67

JO - European Journal of Clinical Nutrition

T2 - European Journal of Clinical Nutrition

JF - European Journal of Clinical Nutrition

SN - 0954-3007

IS - Suppl.

ER -