Yeasts and bacterial biosurfactants as demulsifiers for petroleum derivative in seawater emulsions

Fernanda Cristina P. Rocha e Silva, Bruno Augusto C. Roque, Nathalia Maria P. Rocha e Silva, Raquel D. Rufino, Juliana M. Luna, Valdemir A. Santos, Ibrahim M Banat, Leonie A. Sarubbo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Oil sludge or waste generated in transport, storage or refining forms highly stable mixtures due to the presence and additives with surfactant properties and water forming complex emulsions. Thus, demulsification is necessary to separate this residual oil from the aqueous phase for oil processing and water treatment/disposal. Most used chemical demulsifiers, although effective, are environmental contaminants and do not meet the desired levels of biodegradation. We investigated the application of microbial biosurfactants as potential natural demulsifiers of petroleum derivatives in water emulsions. Biosurfactants crude extracts, produced by yeasts (Candida guilliermondii, Candida lipolytica and Candida sphaerica) and bacteria (Pseudomonas aeruginosa, Pseudomonas cepacia and Bacillus sp.) grown in industrial residues, were tested for demulsification capacity in their crude and pure forms. The best results obtained were for bacterial biosurfactants, which were able to recover about 65% of the seawater emulsified with motor oil compared to 35–40% only for yeasts products. Biosurfactants were also tested with oil-in-water (O/W) and water-in-oil (W/O) kerosene model emulsions. No relationship between interfacial tension, cell hydrophobicity and demulsification ratios was observed with all the biosurfactants tested. Microscopic illustrations of the emulsions in the presence of the biosurfactants showed the aspects of the emulsion and demulsification process. The results obtained demonstrate the potential of these agents as demulsifiers in marine environments.
LanguageEnglish
JournalApplied Microbiology and Biotechnology Express
Volume7
Early online date15 Nov 2017
DOIs
Publication statusE-pub ahead of print - 15 Nov 2017

Fingerprint

emulsion
yeast
petroleum
seawater
oil
water
hydrophobicity
surfactant
marine environment
biodegradation
water treatment
sludge
bacterium
pollutant

Keywords

  • Pseudomonas
  • Bacillus
  • Candida
  • Demulsification
  • Cell hydrophobicity
  • Interfacial tension
  • Oil
  • Environmental contamination

Cite this

Rocha e Silva, F. C. P., Roque, B. A. C., Rocha e Silva, N. M. P., Rufino, R. D., Luna, J. M., Santos, V. A., ... Sarubbo, L. A. (2017). Yeasts and bacterial biosurfactants as demulsifiers for petroleum derivative in seawater emulsions. Applied Microbiology and Biotechnology Express, 7. https://doi.org/10.1186/s13568-017-0499-6
Rocha e Silva, Fernanda Cristina P. ; Roque, Bruno Augusto C. ; Rocha e Silva, Nathalia Maria P. ; Rufino, Raquel D. ; Luna, Juliana M. ; Santos, Valdemir A. ; Banat, Ibrahim M ; Sarubbo, Leonie A. / Yeasts and bacterial biosurfactants as demulsifiers for petroleum derivative in seawater emulsions. In: Applied Microbiology and Biotechnology Express. 2017 ; Vol. 7.
@article{40d08722d3e64c989743b5abcba439b1,
title = "Yeasts and bacterial biosurfactants as demulsifiers for petroleum derivative in seawater emulsions",
abstract = "Oil sludge or waste generated in transport, storage or refining forms highly stable mixtures due to the presence and additives with surfactant properties and water forming complex emulsions. Thus, demulsification is necessary to separate this residual oil from the aqueous phase for oil processing and water treatment/disposal. Most used chemical demulsifiers, although effective, are environmental contaminants and do not meet the desired levels of biodegradation. We investigated the application of microbial biosurfactants as potential natural demulsifiers of petroleum derivatives in water emulsions. Biosurfactants crude extracts, produced by yeasts (Candida guilliermondii, Candida lipolytica and Candida sphaerica) and bacteria (Pseudomonas aeruginosa, Pseudomonas cepacia and Bacillus sp.) grown in industrial residues, were tested for demulsification capacity in their crude and pure forms. The best results obtained were for bacterial biosurfactants, which were able to recover about 65{\%} of the seawater emulsified with motor oil compared to 35–40{\%} only for yeasts products. Biosurfactants were also tested with oil-in-water (O/W) and water-in-oil (W/O) kerosene model emulsions. No relationship between interfacial tension, cell hydrophobicity and demulsification ratios was observed with all the biosurfactants tested. Microscopic illustrations of the emulsions in the presence of the biosurfactants showed the aspects of the emulsion and demulsification process. The results obtained demonstrate the potential of these agents as demulsifiers in marine environments.",
keywords = "Pseudomonas, Bacillus, Candida, Demulsification, Cell hydrophobicity, Interfacial tension, Oil, Environmental contamination",
author = "{Rocha e Silva}, {Fernanda Cristina P.} and Roque, {Bruno Augusto C.} and {Rocha e Silva}, {Nathalia Maria P.} and Rufino, {Raquel D.} and Luna, {Juliana M.} and Santos, {Valdemir A.} and Banat, {Ibrahim M} and Sarubbo, {Leonie A.}",
year = "2017",
month = "11",
day = "15",
doi = "10.1186/s13568-017-0499-6",
language = "English",
volume = "7",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",

}

Yeasts and bacterial biosurfactants as demulsifiers for petroleum derivative in seawater emulsions. / Rocha e Silva, Fernanda Cristina P.; Roque, Bruno Augusto C.; Rocha e Silva, Nathalia Maria P.; Rufino, Raquel D.; Luna, Juliana M.; Santos, Valdemir A.; Banat, Ibrahim M; Sarubbo, Leonie A.

In: Applied Microbiology and Biotechnology Express, Vol. 7, 15.11.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Yeasts and bacterial biosurfactants as demulsifiers for petroleum derivative in seawater emulsions

AU - Rocha e Silva, Fernanda Cristina P.

AU - Roque, Bruno Augusto C.

AU - Rocha e Silva, Nathalia Maria P.

AU - Rufino, Raquel D.

AU - Luna, Juliana M.

AU - Santos, Valdemir A.

AU - Banat, Ibrahim M

AU - Sarubbo, Leonie A.

PY - 2017/11/15

Y1 - 2017/11/15

N2 - Oil sludge or waste generated in transport, storage or refining forms highly stable mixtures due to the presence and additives with surfactant properties and water forming complex emulsions. Thus, demulsification is necessary to separate this residual oil from the aqueous phase for oil processing and water treatment/disposal. Most used chemical demulsifiers, although effective, are environmental contaminants and do not meet the desired levels of biodegradation. We investigated the application of microbial biosurfactants as potential natural demulsifiers of petroleum derivatives in water emulsions. Biosurfactants crude extracts, produced by yeasts (Candida guilliermondii, Candida lipolytica and Candida sphaerica) and bacteria (Pseudomonas aeruginosa, Pseudomonas cepacia and Bacillus sp.) grown in industrial residues, were tested for demulsification capacity in their crude and pure forms. The best results obtained were for bacterial biosurfactants, which were able to recover about 65% of the seawater emulsified with motor oil compared to 35–40% only for yeasts products. Biosurfactants were also tested with oil-in-water (O/W) and water-in-oil (W/O) kerosene model emulsions. No relationship between interfacial tension, cell hydrophobicity and demulsification ratios was observed with all the biosurfactants tested. Microscopic illustrations of the emulsions in the presence of the biosurfactants showed the aspects of the emulsion and demulsification process. The results obtained demonstrate the potential of these agents as demulsifiers in marine environments.

AB - Oil sludge or waste generated in transport, storage or refining forms highly stable mixtures due to the presence and additives with surfactant properties and water forming complex emulsions. Thus, demulsification is necessary to separate this residual oil from the aqueous phase for oil processing and water treatment/disposal. Most used chemical demulsifiers, although effective, are environmental contaminants and do not meet the desired levels of biodegradation. We investigated the application of microbial biosurfactants as potential natural demulsifiers of petroleum derivatives in water emulsions. Biosurfactants crude extracts, produced by yeasts (Candida guilliermondii, Candida lipolytica and Candida sphaerica) and bacteria (Pseudomonas aeruginosa, Pseudomonas cepacia and Bacillus sp.) grown in industrial residues, were tested for demulsification capacity in their crude and pure forms. The best results obtained were for bacterial biosurfactants, which were able to recover about 65% of the seawater emulsified with motor oil compared to 35–40% only for yeasts products. Biosurfactants were also tested with oil-in-water (O/W) and water-in-oil (W/O) kerosene model emulsions. No relationship between interfacial tension, cell hydrophobicity and demulsification ratios was observed with all the biosurfactants tested. Microscopic illustrations of the emulsions in the presence of the biosurfactants showed the aspects of the emulsion and demulsification process. The results obtained demonstrate the potential of these agents as demulsifiers in marine environments.

KW - Pseudomonas

KW - Bacillus

KW - Candida

KW - Demulsification

KW - Cell hydrophobicity

KW - Interfacial tension

KW - Oil

KW - Environmental contamination

U2 - 10.1186/s13568-017-0499-6

DO - 10.1186/s13568-017-0499-6

M3 - Article

VL - 7

JO - Applied Microbiology and Biotechnology

T2 - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

ER -