Wound healing in MIP-1 alpha(-/-) and MCP-1(-/-) mice

QEH Low, IA Drugea, LA Duffner, Daniel Quinn, DN Cook, BJ Rollins, EJ Kovacs, LA DiPietro

    Research output: Contribution to journalArticle

    217 Citations (Scopus)

    Abstract

    A salient feature of normal wound healing is the development and resolution of an acute inflammatory response. Although much is known about the function of inflammatory cells within wounds, little is known about the chemotactic and activation signals that influence this response. As the CC chemokines macrophage inflammatory protein-1 alpha (MEP-1 alpha) and monocyte chemotactic protein-1 (MCP-1) are abundant in acute wounds, wound repair was examined in MIP-1 alpha (-/-) and MCP-1(-/-) mice. Surprisingly, wound re-epithelialization, angiogenesis, and collagen synthesis in MIP-1 alpha (-/-) mice was nearly identical to wildtype controls. In contrast, MCP-1(-/-) mice displayed significantly delayed wound re-epithelialization, with the greatest delay at day 3 after injury (28 +/- 5% versus 79 +/- 14% re-epithelialization, P < 0.005). Wound angiogenesis was also delayed in MCP-1(-/-) mice, with a 48% reduction in capillary density at day 5 after injury. Collagen synthesis was impeded as well, with the wounds of MCP-1(-/-) mice containing significantly less hydroxyproline than those of control mice (25 <plus/minus> 3 versus 50 +/- 8 mug/wound at day 5, P < 0.0001). No change in the number of wound macrophages was observed in MCP-1(-/-) mice, suggesting that monocyte recruitment into wounds is independent of this chemokine. The data suggest that MCP-1 plays a critical role in healing wounds, most likely by influencing the effector state of macrophages and other cell types.
    LanguageEnglish
    Pages457-463
    JournalAMERICAN JOURNAL OF PATHOLOGY
    Volume159
    Issue number2
    DOIs
    Publication statusPublished - Aug 2001

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    Chemokine CCL2
    Re-Epithelialization
    Wound Healing
    Wounds and Injuries
    Macrophages
    Chemokine CCL3
    CC Chemokines
    Chemokines
    Monocytes
    Collagen

    Cite this

    Low, QEH., Drugea, IA., Duffner, LA., Quinn, D., Cook, DN., Rollins, BJ., ... DiPietro, LA. (2001). Wound healing in MIP-1 alpha(-/-) and MCP-1(-/-) mice. AMERICAN JOURNAL OF PATHOLOGY, 159(2), 457-463. https://doi.org/10.1016/S0002-9440(10)61717-8
    Low, QEH ; Drugea, IA ; Duffner, LA ; Quinn, Daniel ; Cook, DN ; Rollins, BJ ; Kovacs, EJ ; DiPietro, LA. / Wound healing in MIP-1 alpha(-/-) and MCP-1(-/-) mice. In: AMERICAN JOURNAL OF PATHOLOGY. 2001 ; Vol. 159, No. 2. pp. 457-463.
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    abstract = "A salient feature of normal wound healing is the development and resolution of an acute inflammatory response. Although much is known about the function of inflammatory cells within wounds, little is known about the chemotactic and activation signals that influence this response. As the CC chemokines macrophage inflammatory protein-1 alpha (MEP-1 alpha) and monocyte chemotactic protein-1 (MCP-1) are abundant in acute wounds, wound repair was examined in MIP-1 alpha (-/-) and MCP-1(-/-) mice. Surprisingly, wound re-epithelialization, angiogenesis, and collagen synthesis in MIP-1 alpha (-/-) mice was nearly identical to wildtype controls. In contrast, MCP-1(-/-) mice displayed significantly delayed wound re-epithelialization, with the greatest delay at day 3 after injury (28 +/- 5{\%} versus 79 +/- 14{\%} re-epithelialization, P < 0.005). Wound angiogenesis was also delayed in MCP-1(-/-) mice, with a 48{\%} reduction in capillary density at day 5 after injury. Collagen synthesis was impeded as well, with the wounds of MCP-1(-/-) mice containing significantly less hydroxyproline than those of control mice (25 3 versus 50 +/- 8 mug/wound at day 5, P < 0.0001). No change in the number of wound macrophages was observed in MCP-1(-/-) mice, suggesting that monocyte recruitment into wounds is independent of this chemokine. The data suggest that MCP-1 plays a critical role in healing wounds, most likely by influencing the effector state of macrophages and other cell types.",
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    Low, QEH, Drugea, IA, Duffner, LA, Quinn, D, Cook, DN, Rollins, BJ, Kovacs, EJ & DiPietro, LA 2001, 'Wound healing in MIP-1 alpha(-/-) and MCP-1(-/-) mice', AMERICAN JOURNAL OF PATHOLOGY, vol. 159, no. 2, pp. 457-463. https://doi.org/10.1016/S0002-9440(10)61717-8

    Wound healing in MIP-1 alpha(-/-) and MCP-1(-/-) mice. / Low, QEH; Drugea, IA; Duffner, LA; Quinn, Daniel; Cook, DN; Rollins, BJ; Kovacs, EJ; DiPietro, LA.

    In: AMERICAN JOURNAL OF PATHOLOGY, Vol. 159, No. 2, 08.2001, p. 457-463.

    Research output: Contribution to journalArticle

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    AB - A salient feature of normal wound healing is the development and resolution of an acute inflammatory response. Although much is known about the function of inflammatory cells within wounds, little is known about the chemotactic and activation signals that influence this response. As the CC chemokines macrophage inflammatory protein-1 alpha (MEP-1 alpha) and monocyte chemotactic protein-1 (MCP-1) are abundant in acute wounds, wound repair was examined in MIP-1 alpha (-/-) and MCP-1(-/-) mice. Surprisingly, wound re-epithelialization, angiogenesis, and collagen synthesis in MIP-1 alpha (-/-) mice was nearly identical to wildtype controls. In contrast, MCP-1(-/-) mice displayed significantly delayed wound re-epithelialization, with the greatest delay at day 3 after injury (28 +/- 5% versus 79 +/- 14% re-epithelialization, P < 0.005). Wound angiogenesis was also delayed in MCP-1(-/-) mice, with a 48% reduction in capillary density at day 5 after injury. Collagen synthesis was impeded as well, with the wounds of MCP-1(-/-) mice containing significantly less hydroxyproline than those of control mice (25 3 versus 50 +/- 8 mug/wound at day 5, P < 0.0001). No change in the number of wound macrophages was observed in MCP-1(-/-) mice, suggesting that monocyte recruitment into wounds is independent of this chemokine. The data suggest that MCP-1 plays a critical role in healing wounds, most likely by influencing the effector state of macrophages and other cell types.

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    Low QEH, Drugea IA, Duffner LA, Quinn D, Cook DN, Rollins BJ et al. Wound healing in MIP-1 alpha(-/-) and MCP-1(-/-) mice. AMERICAN JOURNAL OF PATHOLOGY. 2001 Aug;159(2):457-463. https://doi.org/10.1016/S0002-9440(10)61717-8