Whole blood analysis of phagocytosis, apoptosis, cytokine production, and leukocyte subsets in healthy older men and women: The ZENITH study

Clare F Hodkinson, Jacqueline O'Connor, H Denis Alexander, Ian Bradbury, Maxine P Bonham, BM Hannigan, William S Gilmore, JJ Strain, Julie Wallace

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Abstract

Few studies to date have examined age-related changes in markers of immune status in healthy older individuals. The immune status of 93 healthy individuals aged 55-70 years was assessed by two- and three-color flow cytometry and biochemical analysis. There were significant age effects (p <= .05) on monocyte phagocytic activity and cluster of differentiation (CD) 3/human leukocyte antigen-D-related (HLA-DR) late-activated T lymphocytes (% expression). There was a significant (p <= 0.1) Age X Sex interaction in absolute counts (X 10(9)/L) of CD3/CD8 total cytotoxic T lymphocytes (CTL), the CD4 T-helper to CD8 CTL ratio, the CD3/CD4/CD45RA naive T helper to CD3/CD4/CD45RO memory T helper lymphocyte ratio, and interleukin (1L)-1 beta (% expression) by activated monocytes. The study shows that alterations in markers of immune status occur between 55 and 70 years, and provides reference values for the lymphocyte measures in healthy men and postmenopausal women in this age group. The study further highlights the need for sex-specific reference ranges for such markers.
LanguageEnglish
Pages907-917
JournalJournals of Gerontology, Series A
Volume61
Issue number9
Publication statusPublished - Sep 2006

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Cytotoxic T-Lymphocytes
Phagocytosis
Monocytes
Reference Values
Leukocytes
Color
Biomarkers
CD Antigens
Apoptosis
Cytokines
Helper-Inducer T-Lymphocytes
Interleukin-1beta
Flow Cytometry
Age Groups
Lymphocytes
T-Lymphocytes

Cite this

Hodkinson, C. F., O'Connor, J., Alexander, H. D., Bradbury, I., Bonham, M. P., Hannigan, BM., ... Wallace, J. (2006). Whole blood analysis of phagocytosis, apoptosis, cytokine production, and leukocyte subsets in healthy older men and women: The ZENITH study. Journals of Gerontology, Series A, 61(9), 907-917.
Hodkinson, Clare F ; O'Connor, Jacqueline ; Alexander, H Denis ; Bradbury, Ian ; Bonham, Maxine P ; Hannigan, BM ; Gilmore, William S ; Strain, JJ ; Wallace, Julie. / Whole blood analysis of phagocytosis, apoptosis, cytokine production, and leukocyte subsets in healthy older men and women: The ZENITH study. In: Journals of Gerontology, Series A. 2006 ; Vol. 61, No. 9. pp. 907-917.
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abstract = "Few studies to date have examined age-related changes in markers of immune status in healthy older individuals. The immune status of 93 healthy individuals aged 55-70 years was assessed by two- and three-color flow cytometry and biochemical analysis. There were significant age effects (p <= .05) on monocyte phagocytic activity and cluster of differentiation (CD) 3/human leukocyte antigen-D-related (HLA-DR) late-activated T lymphocytes ({\%} expression). There was a significant (p <= 0.1) Age X Sex interaction in absolute counts (X 10(9)/L) of CD3/CD8 total cytotoxic T lymphocytes (CTL), the CD4 T-helper to CD8 CTL ratio, the CD3/CD4/CD45RA naive T helper to CD3/CD4/CD45RO memory T helper lymphocyte ratio, and interleukin (1L)-1 beta ({\%} expression) by activated monocytes. The study shows that alterations in markers of immune status occur between 55 and 70 years, and provides reference values for the lymphocyte measures in healthy men and postmenopausal women in this age group. The study further highlights the need for sex-specific reference ranges for such markers.",
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Hodkinson, CF, O'Connor, J, Alexander, HD, Bradbury, I, Bonham, MP, Hannigan, BM, Gilmore, WS, Strain, JJ & Wallace, J 2006, 'Whole blood analysis of phagocytosis, apoptosis, cytokine production, and leukocyte subsets in healthy older men and women: The ZENITH study', Journals of Gerontology, Series A, vol. 61, no. 9, pp. 907-917.

Whole blood analysis of phagocytosis, apoptosis, cytokine production, and leukocyte subsets in healthy older men and women: The ZENITH study. / Hodkinson, Clare F; O'Connor, Jacqueline; Alexander, H Denis; Bradbury, Ian; Bonham, Maxine P; Hannigan, BM; Gilmore, William S; Strain, JJ; Wallace, Julie.

In: Journals of Gerontology, Series A, Vol. 61, No. 9, 09.2006, p. 907-917.

Research output: Contribution to journalArticle

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