Objective. This study assessed the workday step counts of lower active (b10,000 daily steps) university 22employees using an automated, web-based walking intervention (Walk@Work). 23Methods. Academic and administrative staff (n=390; 45.6±10.8 years; BMI 27.2±5.5 kg/m2; 290women) 24at five campuses (Australia [x2], Canada, Northern Ireland and the United States), were given a pedometer, 25access to the website program (2010–11) and tasked with increasing workday walking by 1000 daily steps 26above baseline, every two weeks, over a six week period. Step count changes at four weeks post intervention 27were evaluated relative to campus and baseline walking. 28Results. Across the sample, step counts significantly increased frombaseline to post-intervention (1477 daily 29steps; p=0.001). Variations in increases were evident between campuses (largest difference of 870 daily steps; 30p=0.04) and for baseline activity status. Those least active at baseline (b5000 daily steps; n=125) increased 31step counts the most (1837 daily steps; p=0.001), whereas those most active (7500–9999 daily steps; n=79) 32increased the least (929 daily steps; p=0.001). 33Conclusions. Walk@Work increased workday walking by 25% in this sample overall. Increases occurred 34through an automated program, at campuses in different countries, and were most evident for those most in 35need of intervention.