Vitamin D binding protein isoforms as candidate predictors of disease extension in childhood arthritis

David Gibson, Keri Newell, Alexandra Evans, Sorcha Finnegan, Gwen Manning, Caitiona Scaife, Catherine McAllister, Stephen Pennington, Mark Duncan, Terry Moore, Madeleine Rooney

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Abstract

Introduction. Juvenile idiopathic arthritis (JIA) comprises a poorly understood group ofchronic autoimmune diseases with variable clinical outcomes. We investigated whetherthe synovial fluid (SF) proteome could distinguish a subset of patients in whom diseaseextends to affect a large number of joints.Methods. SF samples from 57 patients were obtained around time of initial diagnosis of JIA,labeled with Cy dyes and separated by two-dimensional electrophoresis. Multivariateanalyses were used to isolate a panel of proteins which distinguish patient subgroups.Proteins were identified using MALDI-TOF mass spectrometry with expression verified byimmunochemical methods. Protein glycosylation status was confirmed by hydrophilicinteraction liquid chromatography.Results. A truncated isoform of vitamin D binding protein (VDBP) is present at significantlyreduced levels in the SF of oligoarticular patients at risk of disease extension, relative toother subgroups (p
Original languageEnglish
Pages (from-to)5479-5492
JournalJournal of Proteomics
Volume75
DOIs
Publication statusPublished - 2012

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    Gibson, D., Newell, K., Evans, A., Finnegan, S., Manning, G., Scaife, C., McAllister, C., Pennington, S., Duncan, M., Moore, T., & Rooney, M. (2012). Vitamin D binding protein isoforms as candidate predictors of disease extension in childhood arthritis. Journal of Proteomics, 75, 5479-5492. https://doi.org/10.1016/j.jprot.2012.06.024