TY - JOUR
T1 - Vitamin D binding protein isoforms as candidate predictors of disease extension in childhood arthritis
AU - Gibson, David
AU - Newell, Keri
AU - Evans, Alexandra
AU - Finnegan, Sorcha
AU - Manning, Gwen
AU - Scaife, Caitiona
AU - McAllister, Catherine
AU - Pennington, Stephen
AU - Duncan, Mark
AU - Moore, Terry
AU - Rooney, Madeleine
PY - 2012
Y1 - 2012
N2 - Introduction. Juvenile idiopathic arthritis (JIA) comprises a poorly understood group ofchronic autoimmune diseases with variable clinical outcomes. We investigated whetherthe synovial fluid (SF) proteome could distinguish a subset of patients in whom diseaseextends to affect a large number of joints.Methods. SF samples from 57 patients were obtained around time of initial diagnosis of JIA,labeled with Cy dyes and separated by two-dimensional electrophoresis. Multivariateanalyses were used to isolate a panel of proteins which distinguish patient subgroups.Proteins were identified using MALDI-TOF mass spectrometry with expression verified byimmunochemical methods. Protein glycosylation status was confirmed by hydrophilicinteraction liquid chromatography.Results. A truncated isoform of vitamin D binding protein (VDBP) is present at significantlyreduced levels in the SF of oligoarticular patients at risk of disease extension, relative toother subgroups (p
AB - Introduction. Juvenile idiopathic arthritis (JIA) comprises a poorly understood group ofchronic autoimmune diseases with variable clinical outcomes. We investigated whetherthe synovial fluid (SF) proteome could distinguish a subset of patients in whom diseaseextends to affect a large number of joints.Methods. SF samples from 57 patients were obtained around time of initial diagnosis of JIA,labeled with Cy dyes and separated by two-dimensional electrophoresis. Multivariateanalyses were used to isolate a panel of proteins which distinguish patient subgroups.Proteins were identified using MALDI-TOF mass spectrometry with expression verified byimmunochemical methods. Protein glycosylation status was confirmed by hydrophilicinteraction liquid chromatography.Results. A truncated isoform of vitamin D binding protein (VDBP) is present at significantlyreduced levels in the SF of oligoarticular patients at risk of disease extension, relative toother subgroups (p
U2 - 10.1016/j.jprot.2012.06.024
DO - 10.1016/j.jprot.2012.06.024
M3 - Article
VL - 75
SP - 5479
EP - 5492
JO - Journal of Proteomics
JF - Journal of Proteomics
ER -
