Vitamin D binding protein identified in synovial proteome associated with disease extension in JIA patients

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Aim/introduction: Current laboratory tests are unable to flag juvenile
idiopathic arthritis (JIA) patients at a higher risk of disease spread to
multiple joints, who could benefit from earlier therapy to prevent joint
damage. This study was focused on profiling the synovial fluid (SF)
proteome associated with disease extension from oligo- to polyarticular
status by a difference gel electrophoresis (DIGE) approach.
Method: SF samples from 55 JIA patients were analysed: 30 oligo-, 8
extended oligo- and 17 polyarticular disease. Initial SF samples from
each patient were labeled with Cy dyes and subjected to protein
separation by 2-DE. Protein spots of interest over expressed between
patient subgroups were identified by MALDI-TOF. Specific antibodies
were used to validate putative biomarker expression in
synovial fluid and in synovial membrane (SM).
Results: Samespots software analysis of SF gel scans was used to
highlight differentially expressed proteins. Hierarchical clustering
based on the expression levels of 40 proteins matched across the three
clinical subgroups segregates the extended oligoarticular patients.
Proteolytic fragments of apolipoprotein AII, complement component
C3c, and vitamin D binding protein were identified (p\0.05)
amongst the discriminatory proteins. Apolipoprotein AII and vitamin
D binding protein were expressed at significantly higher levels in the
polyarticular patients, p = 0.046 and p = 0.019, respectively, both
with a perivascular distribution in the SM.
Conclusions: Synovial fluid proteome profiles have been used to flag
JIA patients at risk of disease spread. The panel of identified proteins
may play a role in spread of joint inflammation. With further validation,
these putative prognostic biomarkers could improve the
clinical management of patients.
Original languageEnglish
Title of host publicationIrish Journal of Medical Science
Volume179
EditionS14
DOIs
Publication statusPublished - 2010
EventIRISH SOCIETY FOR RHEUMATOLOGY AND IRISH
RHEUMATOLOGY HEALTH PROFESSIONALS SOCIETY –
AUTUMN SCIENTIFIC MEETING
- Belfast, United Kingdom
Duration: 24 Sep 200925 Sep 2009

Conference

ConferenceIRISH SOCIETY FOR RHEUMATOLOGY AND IRISH
RHEUMATOLOGY HEALTH PROFESSIONALS SOCIETY –
AUTUMN SCIENTIFIC MEETING
CountryUnited Kingdom
CityBelfast
Period24/09/0925/09/09

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