Abstract
Aim/introduction: Current laboratory tests are unable to flag juvenile
idiopathic arthritis (JIA) patients at a higher risk of disease spread to
multiple joints, who could benefit from earlier therapy to prevent joint
damage. This study was focused on profiling the synovial fluid (SF)
proteome associated with disease extension from oligo- to polyarticular
status by a difference gel electrophoresis (DIGE) approach.
Method: SF samples from 55 JIA patients were analysed: 30 oligo-, 8
extended oligo- and 17 polyarticular disease. Initial SF samples from
each patient were labeled with Cy dyes and subjected to protein
separation by 2-DE. Protein spots of interest over expressed between
patient subgroups were identified by MALDI-TOF. Specific antibodies
were used to validate putative biomarker expression in
synovial fluid and in synovial membrane (SM).
Results: Samespots software analysis of SF gel scans was used to
highlight differentially expressed proteins. Hierarchical clustering
based on the expression levels of 40 proteins matched across the three
clinical subgroups segregates the extended oligoarticular patients.
Proteolytic fragments of apolipoprotein AII, complement component
C3c, and vitamin D binding protein were identified (p\0.05)
amongst the discriminatory proteins. Apolipoprotein AII and vitamin
D binding protein were expressed at significantly higher levels in the
polyarticular patients, p = 0.046 and p = 0.019, respectively, both
with a perivascular distribution in the SM.
Conclusions: Synovial fluid proteome profiles have been used to flag
JIA patients at risk of disease spread. The panel of identified proteins
may play a role in spread of joint inflammation. With further validation,
these putative prognostic biomarkers could improve the
clinical management of patients.
idiopathic arthritis (JIA) patients at a higher risk of disease spread to
multiple joints, who could benefit from earlier therapy to prevent joint
damage. This study was focused on profiling the synovial fluid (SF)
proteome associated with disease extension from oligo- to polyarticular
status by a difference gel electrophoresis (DIGE) approach.
Method: SF samples from 55 JIA patients were analysed: 30 oligo-, 8
extended oligo- and 17 polyarticular disease. Initial SF samples from
each patient were labeled with Cy dyes and subjected to protein
separation by 2-DE. Protein spots of interest over expressed between
patient subgroups were identified by MALDI-TOF. Specific antibodies
were used to validate putative biomarker expression in
synovial fluid and in synovial membrane (SM).
Results: Samespots software analysis of SF gel scans was used to
highlight differentially expressed proteins. Hierarchical clustering
based on the expression levels of 40 proteins matched across the three
clinical subgroups segregates the extended oligoarticular patients.
Proteolytic fragments of apolipoprotein AII, complement component
C3c, and vitamin D binding protein were identified (p\0.05)
amongst the discriminatory proteins. Apolipoprotein AII and vitamin
D binding protein were expressed at significantly higher levels in the
polyarticular patients, p = 0.046 and p = 0.019, respectively, both
with a perivascular distribution in the SM.
Conclusions: Synovial fluid proteome profiles have been used to flag
JIA patients at risk of disease spread. The panel of identified proteins
may play a role in spread of joint inflammation. With further validation,
these putative prognostic biomarkers could improve the
clinical management of patients.
Original language | English |
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Title of host publication | Irish Journal of Medical Science |
Volume | 179 |
Edition | S14 |
DOIs | |
Publication status | Published (in print/issue) - 2010 |
Event | IRISH SOCIETY FOR RHEUMATOLOGY AND IRISH RHEUMATOLOGY HEALTH PROFESSIONALS SOCIETY – AUTUMN SCIENTIFIC MEETING - Belfast, United Kingdom Duration: 24 Sept 2009 → 25 Sept 2009 |
Conference
Conference | IRISH SOCIETY FOR RHEUMATOLOGY AND IRISH RHEUMATOLOGY HEALTH PROFESSIONALS SOCIETY – AUTUMN SCIENTIFIC MEETING |
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Country/Territory | United Kingdom |
City | Belfast |
Period | 24/09/09 → 25/09/09 |