Vitacrystallography: Appearance and Development of Cancer-Induced Structural Biomarkers in a Mouse Model

Oleksii Avdieiev; Sergey A. Denisov; Ashkan Ajeer; Lois Adams; Charlene Greenwood; Heather Nesbitt; Keith Thomas; Keith Rogers; Olga Solovyeva; Lev Mourokh; Pavel Lazarev

doi:10.3390/life15060904

Vitacrystallography: Appearance and Development of Cancer-Induced Structural Biomarkers in a Mouse Model

Oleksii Avdieiev
, Sergey A. Denisov
, Ashkan Ajeer
, Lois Adams
, Charlene Greenwood
, Heather Nesbitt
, Keith Thomas
, Keith Rogers
, Olga Solovyeva
, Lev Mourokh
, Pavel Lazarev

Research output: Contribution to journal › Article › peer-review

54 Downloads (Pure)

Abstract

Structural biomarkers determined by X-ray scattering of the tissues can complement conventional diagnostics and provide a pathway for early detection of diseases. In the present study, mouse models were utilized to observe the progression of prostate cancer. We induced cancer in the left lobe of the mouse prostate, whilst the right lobe was left uninoculated. The mice were sacrificed at increasing systematic time points, and lobe samples were subsequently analyzed using X-ray scattering. Control samples were also collected from healthy mice sacrificed at the same time points. This investigation revealed that the ratio between the X-ray scattering peaks associated with the lipids and water can serve as a structural biomarker of cancer, and this biomarker develops as the tumor advances. The obtained cancer trajectory can serve as a baseline for the determination of the disease stage, and the biomarker movement along the trajectory can be evidence of the healing or disease progression.

Original language	English
Article number	904
Pages (from-to)	1-11
Number of pages	11
Journal	Life
Volume	15
Issue number	6
Early online date	3 Jun 2025
DOIs	https://doi.org/10.3390/life15060904
Publication status	Published (in print/issue) - 30 Jun 2025

Bibliographical note

Publisher Copyright:
© 2025 by the authors.

Data Availability Statement

The files with the XRD patterns are available at https://doi.org/10.5
281/zenodo.14751060, published 27 January 2025. The codes for the image processing are available upon request.

Funding

This research was funded by Innovate UK, part of UK Research and Innovation, through the Smart Grant scheme (Grant number 10063925).

Funders	Funder number
Innovate UK	10063925

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

vitacrystallography
X-ray scattering
structural biomarkers
mouse model
prostate cancer
cancer trajectory
Structural Biomarkers
Cancer Trajectory
Vitacrystallography
Mouse Model

Access to Document

10.3390/life15060904

life-15-00904-v2Final published version, 1.96 MBLicence: CC BY

Cite this

@article{aee2c130abed47169a929e73542a0785,

title = "Vitacrystallography: Appearance and Development of Cancer-Induced Structural Biomarkers in a Mouse Model",

abstract = "Structural biomarkers determined by X-ray scattering of the tissues can complement conventional diagnostics and provide a pathway for early detection of diseases. In the present study, mouse models were utilized to observe the progression of prostate cancer. We induced cancer in the left lobe of the mouse prostate, whilst the right lobe was left uninoculated. The mice were sacrificed at increasing systematic time points, and lobe samples were subsequently analyzed using X-ray scattering. Control samples were also collected from healthy mice sacrificed at the same time points. This investigation revealed that the ratio between the X-ray scattering peaks associated with the lipids and water can serve as a structural biomarker of cancer, and this biomarker develops as the tumor advances. The obtained cancer trajectory can serve as a baseline for the determination of the disease stage, and the biomarker movement along the trajectory can be evidence of the healing or disease progression.",

keywords = "vitacrystallography, X-ray scattering, structural biomarkers, mouse model, prostate cancer, cancer trajectory, Structural Biomarkers, Cancer Trajectory, Vitacrystallography, Mouse Model",

author = "Oleksii Avdieiev and Denisov, \{Sergey A.\} and Ashkan Ajeer and Lois Adams and Charlene Greenwood and Heather Nesbitt and Keith Thomas and Keith Rogers and Olga Solovyeva and Lev Mourokh and Pavel Lazarev",

note = "Publisher Copyright: {\textcopyright} 2025 by the authors.",

year = "2025",

month = jun,

day = "30",

doi = "10.3390/life15060904",

language = "English",

volume = "15",

pages = "1--11",

journal = "Life",

issn = "2075-1729",

publisher = "MDPI Multidisciplinary Digital Publishing Institute",

number = "6",

}

TY - JOUR

T1 - Vitacrystallography: Appearance and Development of Cancer-Induced Structural Biomarkers in a Mouse Model

AU - Avdieiev, Oleksii

AU - Denisov, Sergey A.

AU - Ajeer, Ashkan

AU - Adams, Lois

AU - Greenwood, Charlene

AU - Nesbitt, Heather

AU - Thomas, Keith

AU - Rogers, Keith

AU - Solovyeva, Olga

AU - Mourokh, Lev

AU - Lazarev, Pavel

PY - 2025/6/30

Y1 - 2025/6/30

N2 - Structural biomarkers determined by X-ray scattering of the tissues can complement conventional diagnostics and provide a pathway for early detection of diseases. In the present study, mouse models were utilized to observe the progression of prostate cancer. We induced cancer in the left lobe of the mouse prostate, whilst the right lobe was left uninoculated. The mice were sacrificed at increasing systematic time points, and lobe samples were subsequently analyzed using X-ray scattering. Control samples were also collected from healthy mice sacrificed at the same time points. This investigation revealed that the ratio between the X-ray scattering peaks associated with the lipids and water can serve as a structural biomarker of cancer, and this biomarker develops as the tumor advances. The obtained cancer trajectory can serve as a baseline for the determination of the disease stage, and the biomarker movement along the trajectory can be evidence of the healing or disease progression.

AB - Structural biomarkers determined by X-ray scattering of the tissues can complement conventional diagnostics and provide a pathway for early detection of diseases. In the present study, mouse models were utilized to observe the progression of prostate cancer. We induced cancer in the left lobe of the mouse prostate, whilst the right lobe was left uninoculated. The mice were sacrificed at increasing systematic time points, and lobe samples were subsequently analyzed using X-ray scattering. Control samples were also collected from healthy mice sacrificed at the same time points. This investigation revealed that the ratio between the X-ray scattering peaks associated with the lipids and water can serve as a structural biomarker of cancer, and this biomarker develops as the tumor advances. The obtained cancer trajectory can serve as a baseline for the determination of the disease stage, and the biomarker movement along the trajectory can be evidence of the healing or disease progression.

KW - vitacrystallography

KW - X-ray scattering

KW - structural biomarkers

KW - mouse model

KW - prostate cancer

KW - cancer trajectory

KW - Structural Biomarkers

KW - Cancer Trajectory

KW - Vitacrystallography

KW - Mouse Model

UR - https://www.scopus.com/pages/publications/105009153887

U2 - 10.3390/life15060904

DO - 10.3390/life15060904

M3 - Article

C2 - 40566558

SN - 2075-1729

VL - 15

SP - 1

EP - 11

JO - Life

JF - Life

IS - 6

M1 - 904

ER -

Vitacrystallography: Appearance and Development of Cancer-Induced Structural Biomarkers in a Mouse Model

Abstract

Bibliographical note

Data Availability Statement

Funding

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this