Vibrational and AFM studies of adsorption of glycine on DLC and silicon-doped DLC

M Ahmed, JA Byrne, JAD McLaughlin, A Elhissi, DA Phoenix, W Ahmed

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A better understanding of protein adsorption onto surfaces of materials is required to control biocompatibility and bioactivity. Diamond-like carbon (DLC) is known to have excellent biocompatibility. Various samples of a-C:H and silicon-doped a-C:H thin films (Si-DLC) were deposited onto silicon substrates using plasma-enhanced chemical vapour deposition (PECVD). Subsequently, the adsorption of the simplest amino acid glycine onto the surfaces of the thin films was investigated to elucidate the mechanisms involved in protein adhesion. The physicochemical characteristics of the surfaces, before and after adsorption of glycine, were investigated using Raman spectroscopy and atomic force microscopy (AFM). The Raman study highlighted a slight decrease in the I D/I G ratio with increasing the silicon dopant levels. Following exposure to glycine solutions, the presence of bands at ?1735 and ?1200 cm -1 indicates that the adsorption of glycine onto the surfaces has taken place. Glycine was bound to the surfaces via both deprotonated carboxyl and protonated amino groups whilst, as the silicon content in the DLC film increased the adsorption of glycine decreased. AFM analysis showed that the surface roughness increased following exposure to glycine. These results show that at low silicon doping the adsorption of the amino acid was enhanced whilst increased doping levels led to a reduced adsorption compared to undoped DLC. Therefore, doping of DLC may provide an approach to control the protein adsorption. © Springer Science+Business Media, LLC 2011.
LanguageEnglish
Pages1729
JournalJournal of Materials Science
Volume47
Issue number4
DOIs
Publication statusPublished - 2012

Fingerprint

Diamond
Silicon
Glycine
Amino acids
Atomic force microscopy
Diamonds
Carbon
Adsorption
Doping (additives)
Biocompatibility
Proteins
Amino Acids
Thin films
Diamond like carbon films
Plasma enhanced chemical vapor deposition
Bioactivity
Raman spectroscopy
Adhesion
Surface roughness
Substrates

Cite this

@article{b8c447163ce341619d9e43e86299d491,
title = "Vibrational and AFM studies of adsorption of glycine on DLC and silicon-doped DLC",
abstract = "A better understanding of protein adsorption onto surfaces of materials is required to control biocompatibility and bioactivity. Diamond-like carbon (DLC) is known to have excellent biocompatibility. Various samples of a-C:H and silicon-doped a-C:H thin films (Si-DLC) were deposited onto silicon substrates using plasma-enhanced chemical vapour deposition (PECVD). Subsequently, the adsorption of the simplest amino acid glycine onto the surfaces of the thin films was investigated to elucidate the mechanisms involved in protein adhesion. The physicochemical characteristics of the surfaces, before and after adsorption of glycine, were investigated using Raman spectroscopy and atomic force microscopy (AFM). The Raman study highlighted a slight decrease in the I D/I G ratio with increasing the silicon dopant levels. Following exposure to glycine solutions, the presence of bands at ?1735 and ?1200 cm -1 indicates that the adsorption of glycine onto the surfaces has taken place. Glycine was bound to the surfaces via both deprotonated carboxyl and protonated amino groups whilst, as the silicon content in the DLC film increased the adsorption of glycine decreased. AFM analysis showed that the surface roughness increased following exposure to glycine. These results show that at low silicon doping the adsorption of the amino acid was enhanced whilst increased doping levels led to a reduced adsorption compared to undoped DLC. Therefore, doping of DLC may provide an approach to control the protein adsorption. {\circledC} Springer Science+Business Media, LLC 2011.",
author = "M Ahmed and JA Byrne and JAD McLaughlin and A Elhissi and DA Phoenix and W Ahmed",
year = "2012",
doi = "10.1007/s10853-011-5952-3",
language = "English",
volume = "47",
pages = "1729",
journal = "Journal of Materials Science",
issn = "0022-2461",
number = "4",

}

Vibrational and AFM studies of adsorption of glycine on DLC and silicon-doped DLC. / Ahmed, M; Byrne, JA; McLaughlin, JAD; Elhissi, A; Phoenix, DA; Ahmed, W.

In: Journal of Materials Science, Vol. 47, No. 4, 2012, p. 1729.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Vibrational and AFM studies of adsorption of glycine on DLC and silicon-doped DLC

AU - Ahmed, M

AU - Byrne, JA

AU - McLaughlin, JAD

AU - Elhissi, A

AU - Phoenix, DA

AU - Ahmed, W

PY - 2012

Y1 - 2012

N2 - A better understanding of protein adsorption onto surfaces of materials is required to control biocompatibility and bioactivity. Diamond-like carbon (DLC) is known to have excellent biocompatibility. Various samples of a-C:H and silicon-doped a-C:H thin films (Si-DLC) were deposited onto silicon substrates using plasma-enhanced chemical vapour deposition (PECVD). Subsequently, the adsorption of the simplest amino acid glycine onto the surfaces of the thin films was investigated to elucidate the mechanisms involved in protein adhesion. The physicochemical characteristics of the surfaces, before and after adsorption of glycine, were investigated using Raman spectroscopy and atomic force microscopy (AFM). The Raman study highlighted a slight decrease in the I D/I G ratio with increasing the silicon dopant levels. Following exposure to glycine solutions, the presence of bands at ?1735 and ?1200 cm -1 indicates that the adsorption of glycine onto the surfaces has taken place. Glycine was bound to the surfaces via both deprotonated carboxyl and protonated amino groups whilst, as the silicon content in the DLC film increased the adsorption of glycine decreased. AFM analysis showed that the surface roughness increased following exposure to glycine. These results show that at low silicon doping the adsorption of the amino acid was enhanced whilst increased doping levels led to a reduced adsorption compared to undoped DLC. Therefore, doping of DLC may provide an approach to control the protein adsorption. © Springer Science+Business Media, LLC 2011.

AB - A better understanding of protein adsorption onto surfaces of materials is required to control biocompatibility and bioactivity. Diamond-like carbon (DLC) is known to have excellent biocompatibility. Various samples of a-C:H and silicon-doped a-C:H thin films (Si-DLC) were deposited onto silicon substrates using plasma-enhanced chemical vapour deposition (PECVD). Subsequently, the adsorption of the simplest amino acid glycine onto the surfaces of the thin films was investigated to elucidate the mechanisms involved in protein adhesion. The physicochemical characteristics of the surfaces, before and after adsorption of glycine, were investigated using Raman spectroscopy and atomic force microscopy (AFM). The Raman study highlighted a slight decrease in the I D/I G ratio with increasing the silicon dopant levels. Following exposure to glycine solutions, the presence of bands at ?1735 and ?1200 cm -1 indicates that the adsorption of glycine onto the surfaces has taken place. Glycine was bound to the surfaces via both deprotonated carboxyl and protonated amino groups whilst, as the silicon content in the DLC film increased the adsorption of glycine decreased. AFM analysis showed that the surface roughness increased following exposure to glycine. These results show that at low silicon doping the adsorption of the amino acid was enhanced whilst increased doping levels led to a reduced adsorption compared to undoped DLC. Therefore, doping of DLC may provide an approach to control the protein adsorption. © Springer Science+Business Media, LLC 2011.

U2 - 10.1007/s10853-011-5952-3

DO - 10.1007/s10853-011-5952-3

M3 - Article

VL - 47

SP - 1729

JO - Journal of Materials Science

T2 - Journal of Materials Science

JF - Journal of Materials Science

SN - 0022-2461

IS - 4

ER -