Verification of cell viability at progressively higher scanning forces using a hybrid atomic force and fluorescence microscope.

Clifford Barnes, Barry O'Hagan, Vyvyan Howard, G McKerr

Research output: Contribution to journalArticle

Abstract

The prudent use of the atomic force microscope as a supra-vital live cell imaging tool requires that cell viability must be determined before and after scanning. Complementary optical techniques in conjunction with the fluorescent dyes rhodamine-123 and ethidium homodimer have been used within this study to determine cell viability after increasing loads are applied in contact mode. Guideline force ranges for five commonly cultured cell lines, human squamous carcinoma (A431), fibroblast, HeLa, Potorous tridactylis (PtK2) and rat intestinal epithelial (RIE) cells are given.
Original languageEnglish
Pages (from-to)185-9
JournalJournal of Microscopy
Volume228
Issue numberPt 2
Publication statusPublished - 2007

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