Verification of cell viability at progressively higher scanning forces using a hybrid atomic force and fluorescence microscope.

Clifford Barnes, Barry O'Hagan, Vyvyan Howard, G McKerr

Research output: Contribution to journalArticle

Abstract

The prudent use of the atomic force microscope as a supra-vital live cell imaging tool requires that cell viability must be determined before and after scanning. Complementary optical techniques in conjunction with the fluorescent dyes rhodamine-123 and ethidium homodimer have been used within this study to determine cell viability after increasing loads are applied in contact mode. Guideline force ranges for five commonly cultured cell lines, human squamous carcinoma (A431), fibroblast, HeLa, Potorous tridactylis (PtK2) and rat intestinal epithelial (RIE) cells are given.
LanguageEnglish
Pages185-9
JournalJournal of Microscopy
Volume228
Issue numberPt 2
Publication statusPublished - 2007

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Cell Survival
Fluorescence
Rhodamine 123
Fluorescent Dyes
Squamous Cell Carcinoma
Cultured Cells
Fibroblasts
Epithelial Cells
Guidelines
Cell Line
ethidium homodimer

Cite this

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abstract = "The prudent use of the atomic force microscope as a supra-vital live cell imaging tool requires that cell viability must be determined before and after scanning. Complementary optical techniques in conjunction with the fluorescent dyes rhodamine-123 and ethidium homodimer have been used within this study to determine cell viability after increasing loads are applied in contact mode. Guideline force ranges for five commonly cultured cell lines, human squamous carcinoma (A431), fibroblast, HeLa, Potorous tridactylis (PtK2) and rat intestinal epithelial (RIE) cells are given.",
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Verification of cell viability at progressively higher scanning forces using a hybrid atomic force and fluorescence microscope. / Barnes, Clifford; O'Hagan, Barry; Howard, Vyvyan; McKerr, G.

In: Journal of Microscopy, Vol. 228, No. Pt 2, 2007, p. 185-9.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Verification of cell viability at progressively higher scanning forces using a hybrid atomic force and fluorescence microscope.

AU - Barnes, Clifford

AU - O'Hagan, Barry

AU - Howard, Vyvyan

AU - McKerr, G

PY - 2007

Y1 - 2007

N2 - The prudent use of the atomic force microscope as a supra-vital live cell imaging tool requires that cell viability must be determined before and after scanning. Complementary optical techniques in conjunction with the fluorescent dyes rhodamine-123 and ethidium homodimer have been used within this study to determine cell viability after increasing loads are applied in contact mode. Guideline force ranges for five commonly cultured cell lines, human squamous carcinoma (A431), fibroblast, HeLa, Potorous tridactylis (PtK2) and rat intestinal epithelial (RIE) cells are given.

AB - The prudent use of the atomic force microscope as a supra-vital live cell imaging tool requires that cell viability must be determined before and after scanning. Complementary optical techniques in conjunction with the fluorescent dyes rhodamine-123 and ethidium homodimer have been used within this study to determine cell viability after increasing loads are applied in contact mode. Guideline force ranges for five commonly cultured cell lines, human squamous carcinoma (A431), fibroblast, HeLa, Potorous tridactylis (PtK2) and rat intestinal epithelial (RIE) cells are given.

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JO - Journal of Microscopy

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