Valosin: isolation and characterization of a novel peptide from porcine intestine

Wolfgang E. Schmidt; Viktor Mutt; Mats Carlquist; Hartmut Kratzin; J. Michael Conlon; Werner Creutzfeldt

doi:10.1016/0014-5793(85)80021-1

Valosin: isolation and characterization of a novel peptide from porcine intestine

Wolfgang E. Schmidt
, Viktor Mutt
, Mats Carlquist
, Hartmut Kratzin
, J. Michael Conlon
, Werner Creutzfeldt

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

The isolation and primary structure of a novel gastrointestinal peptide, designated valosin, is described. The peptide was purified from porcine upper gut extracts using an HPLC and N-terminal sequence screening strategy which depends on Chromatographic and structural characteristics as isolation criterion. The amino acid sequence of this peptide consists of 25 amino acid residues: V Q Y P V E H P D K F L K F G M T P S K G V L F Y. It does not show structural homology to known protein or nucleotide sequences. According to preliminary experiments, valosin possesses biological effects on the gastrointestinal tract of the dog.

Original language	English
Pages (from-to)	264-268
Number of pages	5
Journal	FEBS Letters
Volume	191
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(85)80021-1
Publication status	Published (in print/issue) - 28 Oct 1985

Funding

We thank Dr R.F. Doolittle, University of California, San Diego, for performing the sequence homology search, Professor T.W. Schwartz and Dr H.F. Hansen, Copenhagen,f or the gas-phases equencingo f peptideT 4, Professor S. Konturek, Krakow, for biological screeninge x-periments and Professor N. Hilschmann, Got-tingen, for providing generouss upport in structural work. Excellentt echnicala ssistanceb y MS E. Rabbe, MS B. Wehle, MS D. Michels, Mrs L. Melin and Mrs B. Agerberth is gratefully acknowledged. This study was supported by Deutsche Forschungsgemeinscha(fgt rant Cr 201 19-4), by the SwedishM edical ResearchC ouncil (grant 13X01010),b y Stiftung Volkswagenwerk and by Smith Kline DauelsbergF oundation.

Funders	Funder number
13X01010
Deutsche Forschungsgemeinschaft DFG (J.L.)	Cr 201 19-4

Keywords

Gastrointestinal peptide
HPLC-sequence screening Primary structure
Isolation
Porcine gut extract
Valosin

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10.1016/0014-5793(85)80021-1

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title = "Valosin: isolation and characterization of a novel peptide from porcine intestine",

abstract = "The isolation and primary structure of a novel gastrointestinal peptide, designated valosin, is described. The peptide was purified from porcine upper gut extracts using an HPLC and N-terminal sequence screening strategy which depends on Chromatographic and structural characteristics as isolation criterion. The amino acid sequence of this peptide consists of 25 amino acid residues: V Q Y P V E H P D K F L K F G M T P S K G V L F Y. It does not show structural homology to known protein or nucleotide sequences. According to preliminary experiments, valosin possesses biological effects on the gastrointestinal tract of the dog.",

keywords = "Gastrointestinal peptide, HPLC-sequence screening Primary structure, Isolation, Porcine gut extract, Valosin",

author = "Schmidt, \{Wolfgang E.\} and Viktor Mutt and Mats Carlquist and Hartmut Kratzin and Conlon, \{J. Michael\} and Werner Creutzfeldt",

year = "1985",

month = oct,

day = "28",

doi = "10.1016/0014-5793(85)80021-1",

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AU - Schmidt, Wolfgang E.

AU - Mutt, Viktor

AU - Carlquist, Mats

AU - Kratzin, Hartmut

AU - Conlon, J. Michael

AU - Creutzfeldt, Werner

PY - 1985/10/28

Y1 - 1985/10/28

N2 - The isolation and primary structure of a novel gastrointestinal peptide, designated valosin, is described. The peptide was purified from porcine upper gut extracts using an HPLC and N-terminal sequence screening strategy which depends on Chromatographic and structural characteristics as isolation criterion. The amino acid sequence of this peptide consists of 25 amino acid residues: V Q Y P V E H P D K F L K F G M T P S K G V L F Y. It does not show structural homology to known protein or nucleotide sequences. According to preliminary experiments, valosin possesses biological effects on the gastrointestinal tract of the dog.

AB - The isolation and primary structure of a novel gastrointestinal peptide, designated valosin, is described. The peptide was purified from porcine upper gut extracts using an HPLC and N-terminal sequence screening strategy which depends on Chromatographic and structural characteristics as isolation criterion. The amino acid sequence of this peptide consists of 25 amino acid residues: V Q Y P V E H P D K F L K F G M T P S K G V L F Y. It does not show structural homology to known protein or nucleotide sequences. According to preliminary experiments, valosin possesses biological effects on the gastrointestinal tract of the dog.

KW - Gastrointestinal peptide

KW - HPLC-sequence screening Primary structure

KW - Isolation

KW - Porcine gut extract

KW - Valosin

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AN - SCOPUS:0022375050

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JO - FEBS Letters

JF - FEBS Letters

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ER -

Valosin: isolation and characterization of a novel peptide from porcine intestine

Abstract

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Keywords

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