Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses

Rhonda M Curran, Louise Donnelly, Ryan J Morrow, Carole Fraser, Gavin P Andrews, Martin Cranage, R Karl Malcolm, Robin J Shattock, A David Woolfson

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrixforming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24 h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.
Original languageEnglish
Pages (from-to)6791-6798
JournalVaccine
Volume27
DOIs
Publication statusPublished (in print/issue) - 2009

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