Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses

Rhonda M Curran, Louise Donnelly, Ryan J Morrow, Carole Fraser, Gavin P Andrews, Martin Cranage, R Karl Malcolm, Robin J Shattock, A David Woolfson

    Research output: Contribution to journalArticle

    32 Citations (Scopus)

    Abstract

    Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrixforming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24 h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.
    LanguageEnglish
    Pages6791-6798
    JournalVaccine
    Volume27
    DOIs
    Publication statusPublished - 2009

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    HIV-1
    Immunoglobulin G
    Intravaginal Administration
    Immunoglobulin A
    Immunization
    Glycoproteins
    Polymers
    Vaccination
    Gels
    Enzyme-Linked Immunosorbent Assay
    Rabbits
    Serum
    GP 140
    In Vitro Techniques

    Cite this

    Curran, Rhonda M ; Donnelly, Louise ; Morrow, Ryan J ; Fraser, Carole ; Andrews, Gavin P ; Cranage, Martin ; Malcolm, R Karl ; Shattock, Robin J ; Woolfson, A David. / Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses. 2009 ; Vol. 27. pp. 6791-6798.
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    abstract = "Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrixforming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24 h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.",
    author = "Curran, {Rhonda M} and Louise Donnelly and Morrow, {Ryan J} and Carole Fraser and Andrews, {Gavin P} and Martin Cranage and Malcolm, {R Karl} and Shattock, {Robin J} and Woolfson, {A David}",
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    Curran, RM, Donnelly, L, Morrow, RJ, Fraser, C, Andrews, GP, Cranage, M, Malcolm, RK, Shattock, RJ & Woolfson, AD 2009, 'Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses', vol. 27, pp. 6791-6798. https://doi.org/10.1016/j.vaccine.2009.08.088

    Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses. / Curran, Rhonda M; Donnelly, Louise; Morrow, Ryan J; Fraser, Carole; Andrews, Gavin P; Cranage, Martin; Malcolm, R Karl; Shattock, Robin J; Woolfson, A David.

    Vol. 27, 2009, p. 6791-6798.

    Research output: Contribution to journalArticle

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    AU - Curran, Rhonda M

    AU - Donnelly, Louise

    AU - Morrow, Ryan J

    AU - Fraser, Carole

    AU - Andrews, Gavin P

    AU - Cranage, Martin

    AU - Malcolm, R Karl

    AU - Shattock, Robin J

    AU - Woolfson, A David

    PY - 2009

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    AB - Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrixforming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24 h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.

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