Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses

Rhonda M Curran; Louise Donnelly; Ryan J Morrow; Carole Fraser; Gavin P Andrews; Martin Cranage; R Karl Malcolm; Robin J Shattock; A David Woolfson

doi:10.1016/j.vaccine.2009.08.088

Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses

Rhonda M Curran, Louise Donnelly, Ryan J Morrow, Carole Fraser, Gavin P Andrews, Martin Cranage, R Karl Malcolm, Robin J Shattock, A David Woolfson

Centre for Maternal, Fetal and Infant Research (MFIR)

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrixforming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24 h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.

Original language	English
Pages (from-to)	6791-6798
Journal	Vaccine
Volume	27
DOIs	https://doi.org/10.1016/j.vaccine.2009.08.088
Publication status	Published (in print/issue) - 2009

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Curran, R. M., Donnelly, L., Morrow, R. J., Fraser, C., Andrews, G. P., Cranage, M., Malcolm, R. K., Shattock, R. J., & Woolfson, A. D. (2009). Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses. Vaccine, 27, 6791-6798. https://doi.org/10.1016/j.vaccine.2009.08.088

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title = "Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses",

abstract = "Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrixforming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24 h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.",

author = "Curran, {Rhonda M} and Louise Donnelly and Morrow, {Ryan J} and Carole Fraser and Andrews, {Gavin P} and Martin Cranage and Malcolm, {R Karl} and Shattock, {Robin J} and Woolfson, {A David}",

year = "2009",

doi = "10.1016/j.vaccine.2009.08.088",

language = "English",

volume = "27",

pages = "6791--6798",

journal = "Vaccine",

publisher = "Elsevier Ltd",

}

Curran, RM, Donnelly, L, Morrow, RJ, Fraser, C, Andrews, GP, Cranage, M, Malcolm, RK, Shattock, RJ & Woolfson, AD 2009, 'Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses', Vaccine, vol. 27, pp. 6791-6798. https://doi.org/10.1016/j.vaccine.2009.08.088

TY - JOUR

T1 - Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses

AU - Curran, Rhonda M

AU - Donnelly, Louise

AU - Morrow, Ryan J

AU - Fraser, Carole

AU - Andrews, Gavin P

AU - Cranage, Martin

AU - Malcolm, R Karl

AU - Shattock, Robin J

AU - Woolfson, A David

PY - 2009

Y1 - 2009

N2 - Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrixforming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24 h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.

AB - Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrixforming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24 h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.

U2 - 10.1016/j.vaccine.2009.08.088

DO - 10.1016/j.vaccine.2009.08.088

M3 - Article

VL - 27

SP - 6791

EP - 6798

JO - Vaccine

JF - Vaccine

ER -

Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses

Abstract

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