Abstract
| Language | English |
|---|---|
| Pages | 6791-6798 |
| Journal | Vaccine |
| Volume | 27 |
| DOIs | |
| Publication status | Published - 2009 |
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Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses. / Curran, Rhonda M; Donnelly, Louise; Morrow, Ryan J; Fraser, Carole; Andrews, Gavin P; Cranage, Martin; Malcolm, R Karl; Shattock, Robin J; Woolfson, A David.
In: Vaccine, Vol. 27, 2009, p. 6791-6798.Research output: Contribution to journal › Article
TY - JOUR
T1 - Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelopeprotein CN54gp140 within novel rheologically structured vehicles elicitsspecific immune responses
AU - Curran, Rhonda M
AU - Donnelly, Louise
AU - Morrow, Ryan J
AU - Fraser, Carole
AU - Andrews, Gavin P
AU - Cranage, Martin
AU - Malcolm, R Karl
AU - Shattock, Robin J
AU - Woolfson, A David
PY - 2009
Y1 - 2009
N2 - Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrixforming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24 h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.
AB - Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrixforming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24 h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.
U2 - 10.1016/j.vaccine.2009.08.088
DO - 10.1016/j.vaccine.2009.08.088
M3 - Article
VL - 27
SP - 6791
EP - 6798
JO - Vaccine
T2 - Vaccine
JF - Vaccine
SN - 0264-410X
ER -