Unmasking venom gland transcriptomes in reptile venoms

TB Chen, AJ Bjourson, DF Orr, H Kwok, PF Rao, C Ivanyi, C Shaw

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

While structural studies of reptile venom toxins can be achieved using lyophilized venom samples, until now the cloning of precursor cDNAs required sacrifice of the specimen for dissection of the venom glands. Here we describe a simple and rapid technique that unmasks venom protein mRNAs present in lyophilized venom samples. To illustrate the technique we have RT-PCR-amplified a range of venom protein transcripts from cDNA libraries derived from the venoms of a hemotoxic snake, the Chinese copperhead (Deinagkistrodon acutus), a neurotoxic snake, the black mamba (Dendroaspis polylepis), and a venomous lizard, the Gila monster (Heloderma suspectum). These include a metalloproteinase and phospholipase A2 from D. acutus, a potassium channel blocker, dendrotoxin K, from D. polylepis, and exendin-4 from H. suspectum. These findings imply that the apparent absence and/or lability of mRNA in complex biological matrices is not always real and paves the way for accelerated acquisition of molecular genetic data on venom toxins for scientific and potential therapeutic purposes without sacrifice of endangered herpetofauna. (C) 2002 Elsevier Science (USA). All rights reserved.
LanguageEnglish
Pages152-156
JournalAnalytical Biochemistry
Volume311
Issue number2
Publication statusPublished - Dec 2002

Fingerprint

venoms
transcriptome
reptiles
snakes
toxins
herpetofauna
phospholipase A2
potassium channels
metalloproteinases
cDNA libraries
molecular genetics
lizards
molecular cloning
proteins
reverse transcriptase polymerase chain reaction
sampling
therapeutics
methodology
Heloderma suspectum

Cite this

Chen, TB., Bjourson, AJ., Orr, DF., Kwok, H., Rao, PF., Ivanyi, C., & Shaw, C. (2002). Unmasking venom gland transcriptomes in reptile venoms. Analytical Biochemistry, 311(2), 152-156.
Chen, TB ; Bjourson, AJ ; Orr, DF ; Kwok, H ; Rao, PF ; Ivanyi, C ; Shaw, C. / Unmasking venom gland transcriptomes in reptile venoms. In: Analytical Biochemistry. 2002 ; Vol. 311, No. 2. pp. 152-156.
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Chen, TB, Bjourson, AJ, Orr, DF, Kwok, H, Rao, PF, Ivanyi, C & Shaw, C 2002, 'Unmasking venom gland transcriptomes in reptile venoms', Analytical Biochemistry, vol. 311, no. 2, pp. 152-156.

Unmasking venom gland transcriptomes in reptile venoms. / Chen, TB; Bjourson, AJ; Orr, DF; Kwok, H; Rao, PF; Ivanyi, C; Shaw, C.

In: Analytical Biochemistry, Vol. 311, No. 2, 12.2002, p. 152-156.

Research output: Contribution to journalArticle

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Chen TB, Bjourson AJ, Orr DF, Kwok H, Rao PF, Ivanyi C et al. Unmasking venom gland transcriptomes in reptile venoms. Analytical Biochemistry. 2002 Dec;311(2):152-156.