Ultrasound-assisted synthesis of chitosan from fungal precursors for biomedical applications

Li Fang Zhu, Jing Song Li, John Mai, Ming-Wei Chang

Research output: Contribution to journalArticle

Abstract

Chitin, from the fungal source (Ganoderma lucidum spore powders, GLSP), was converted into chitosan via dual-frequency ultrasound irradiation which produced enhanced results compared with single-frequency processing. The differences between the effects of dual-frequency ultrasound irradiation (at 15 kHz and 20 kHz) with two superposition modes, in orthogonal and parallel orientations, were studied. SEM images confirmed morphology change in the presence of the dual-frequency sources. The enhancement of the degree of deacetylation (DD), dynamic viscosity ([η]) and molecular weight (Mv¯) of the resultant chitosan were also improved by the orthogonal configuration for dual-frequency ultrasound irradiation. In addition, the FTIR, TGA, XRD and 13 C NMR results show the differences in chemical groups, thermal stability and crystalline using two different ultrasound conditions in detail. The resulting biocompatible sample improved the proliferation of L929 cells, while antibacterial activity was also observed using E. coli and S. aureus. This presents a promising new use of a fungal material for biomedical applications.
LanguageEnglish
Pages498-507
Number of pages10
JournalChemical Engineering Journal
Volume357
Early online date25 Sep 2018
DOIs
Publication statusPublished - 1 Feb 2019

Fingerprint

Chitosan
Ultrasonics
irradiation
Irradiation
Chitin
chitin
Powders
Escherichia coli
nuclear magnetic resonance
spore
Thermodynamic stability
viscosity
Molecular weight
scanning electron microscopy
X-ray diffraction
Nuclear magnetic resonance
Viscosity
Crystalline materials
Scanning electron microscopy
ultrasound

Keywords

  • Chitin
  • Chitosan
  • Biomaterial
  • Ultrasound
  • Dual-frequency

Cite this

Zhu, Li Fang ; Li, Jing Song ; Mai, John ; Chang, Ming-Wei. / Ultrasound-assisted synthesis of chitosan from fungal precursors for biomedical applications. 2019 ; Vol. 357. pp. 498-507.
@article{d680ecbc770a4f7989ccf83b74058b3a,
title = "Ultrasound-assisted synthesis of chitosan from fungal precursors for biomedical applications",
abstract = "Chitin, from the fungal source (Ganoderma lucidum spore powders, GLSP), was converted into chitosan via dual-frequency ultrasound irradiation which produced enhanced results compared with single-frequency processing. The differences between the effects of dual-frequency ultrasound irradiation (at 15 kHz and 20 kHz) with two superposition modes, in orthogonal and parallel orientations, were studied. SEM images confirmed morphology change in the presence of the dual-frequency sources. The enhancement of the degree of deacetylation (DD), dynamic viscosity ([η]) and molecular weight (Mv¯) of the resultant chitosan were also improved by the orthogonal configuration for dual-frequency ultrasound irradiation. In addition, the FTIR, TGA, XRD and 13 C NMR results show the differences in chemical groups, thermal stability and crystalline using two different ultrasound conditions in detail. The resulting biocompatible sample improved the proliferation of L929 cells, while antibacterial activity was also observed using E. coli and S. aureus. This presents a promising new use of a fungal material for biomedical applications.",
keywords = "Chitin, Chitosan, Biomaterial, Ultrasound, Dual-frequency",
author = "Zhu, {Li Fang} and Li, {Jing Song} and John Mai and Ming-Wei Chang",
note = "Ming-Wei Chang was employed in China at the time of acceptance and publication and was therefore not aware of or bound by open access rules at the time.",
year = "2019",
month = "2",
day = "1",
doi = "10.1016/j.cej.2018.09.183",
language = "English",
volume = "357",
pages = "498--507",

}

Ultrasound-assisted synthesis of chitosan from fungal precursors for biomedical applications. / Zhu, Li Fang; Li, Jing Song; Mai, John; Chang, Ming-Wei.

Vol. 357, 01.02.2019, p. 498-507.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ultrasound-assisted synthesis of chitosan from fungal precursors for biomedical applications

AU - Zhu, Li Fang

AU - Li, Jing Song

AU - Mai, John

AU - Chang, Ming-Wei

N1 - Ming-Wei Chang was employed in China at the time of acceptance and publication and was therefore not aware of or bound by open access rules at the time.

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Chitin, from the fungal source (Ganoderma lucidum spore powders, GLSP), was converted into chitosan via dual-frequency ultrasound irradiation which produced enhanced results compared with single-frequency processing. The differences between the effects of dual-frequency ultrasound irradiation (at 15 kHz and 20 kHz) with two superposition modes, in orthogonal and parallel orientations, were studied. SEM images confirmed morphology change in the presence of the dual-frequency sources. The enhancement of the degree of deacetylation (DD), dynamic viscosity ([η]) and molecular weight (Mv¯) of the resultant chitosan were also improved by the orthogonal configuration for dual-frequency ultrasound irradiation. In addition, the FTIR, TGA, XRD and 13 C NMR results show the differences in chemical groups, thermal stability and crystalline using two different ultrasound conditions in detail. The resulting biocompatible sample improved the proliferation of L929 cells, while antibacterial activity was also observed using E. coli and S. aureus. This presents a promising new use of a fungal material for biomedical applications.

AB - Chitin, from the fungal source (Ganoderma lucidum spore powders, GLSP), was converted into chitosan via dual-frequency ultrasound irradiation which produced enhanced results compared with single-frequency processing. The differences between the effects of dual-frequency ultrasound irradiation (at 15 kHz and 20 kHz) with two superposition modes, in orthogonal and parallel orientations, were studied. SEM images confirmed morphology change in the presence of the dual-frequency sources. The enhancement of the degree of deacetylation (DD), dynamic viscosity ([η]) and molecular weight (Mv¯) of the resultant chitosan were also improved by the orthogonal configuration for dual-frequency ultrasound irradiation. In addition, the FTIR, TGA, XRD and 13 C NMR results show the differences in chemical groups, thermal stability and crystalline using two different ultrasound conditions in detail. The resulting biocompatible sample improved the proliferation of L929 cells, while antibacterial activity was also observed using E. coli and S. aureus. This presents a promising new use of a fungal material for biomedical applications.

KW - Chitin

KW - Chitosan

KW - Biomaterial

KW - Ultrasound

KW - Dual-frequency

UR - http://www.scopus.com/inward/record.url?scp=85054073746&partnerID=8YFLogxK

U2 - 10.1016/j.cej.2018.09.183

DO - 10.1016/j.cej.2018.09.183

M3 - Article

VL - 357

SP - 498

EP - 507

ER -