UHPLC-HRMS Spectrometric Analysis: Method Validation and Plasma and Urinary Metabolite Identification after Mango Pulp Intake

Salud Cáceres-Jiménez; Raquel Rodríguez-Solana; Sara Dobani; Kirsty Pourshahidi; Chris Gill; José Manuel Moreno-Rojas; Tahani M. Almutairi; Alan Crozier; Gema Pereira-Caro

doi:10.1021/acs.jafc.3c03846

UHPLC-HRMS Spectrometric Analysis: Method Validation and Plasma and Urinary Metabolite Identification after Mango Pulp Intake

Salud Cáceres-Jiménez
, Raquel Rodríguez-Solana
, Sara Dobani
, Kirsty Pourshahidi
, Chris Gill
, José Manuel Moreno-Rojas
, Tahani M. Almutairi
, Alan Crozier
, Gema Pereira-Caro

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

115 Downloads (Pure)

Abstract

After an acute intake of 300 g of mango purée by 10 subjects, 0 and 24 h urine and plasma samples were analyzed by high-performance liquid chromatography–high-resolution mass spectrometry. The method was first validated for 44 reference polyphenols in terms of linearity, specificity, limits of detection and quantification, intra-day and inter-day precision, recovery, and matrix effects in two biological matrices. After method validation, a total of 94 microbial-derived phenolic catabolites, including 15 cinnamic acids, 3 phenylhydracrylic acids, 14 phenylpropanoic acids, 12 phenylacetic acids, 28 benzoic acids, 2 mandelic acids, 15 hydroxybenzenes, and 5 hippuric acid derivatives, were identified or tentatively identified in urine and/or plasma. These results establish the value of the UHPLC-HRMS protocol and the use of authentic standards to obtain a detailed and accurate picture of mango polyphenol metabolites, together with their phase II conjugated metabolites, in human bioavailability studies.

Original language	English
Pages (from-to)	11520-11533
Number of pages	14
Journal	Journal of Agricultural and Food Chemistry
Volume	71
Issue number	30
Early online date	20 Jul 2023
DOIs	https://doi.org/10.1021/acs.jafc.3c03846
Publication status	Published (in print/issue) - 2 Aug 2023

Bibliographical note

Funding Information:
This study was supported by the project “Bioavailability of mango polyphenols in healthy and ileostomy patients: relevance of the bioactive forms in vivo on the gastrointestinal health” (RTI2018-096703-J-I00), funded by the European Regional Development Funds (ERDF), the Spanish Ministry of Science, Innovation and Universities (MCIU), and the Spanish State Research Agency (AEI). S.C.-J. was supported by a predoctoral fellowship funded by the Spanish Ministry of Universities (FPU20/03549). R.R.S. was supported by a Juan de la Cierva–Incorporation contract from the Spanish Ministry of Science, Innovation and Universities (IJC2018-036207-I). A.C. and T.M.A. were supported by the Distinguished Scientist Fellowship Program (DSFP) at King Saud University, Riyadh, Saudi Arabia.

Publisher Copyright:
© 2023 American Chemical Society.

Funding

Funding Information: This study was supported by the project “Bioavailability of mango polyphenols in healthy and ileostomy patients: relevance of the bioactive forms in vivo on the gastrointestinal health” (RTI2018-096703-J-I00), funded by the European Regional Development Funds (ERDF), the Spanish Ministry of Science, Innovation and Universities (MCIU), and the Spanish State Research Agency (AEI). S.C.-J. was supported by a predoctoral fellowship funded by the Spanish Ministry of Universities (FPU20/03549). R.R.S. was supported by a Juan de la Cierva–Incorporation contract from the Spanish Ministry of Science, Innovation and Universities (IJC2018-036207-I). A.C. and T.M.A. were supported by the Distinguished Scientist Fellowship Program (DSFP) at King Saud University, Riyadh, Saudi Arabia. Publisher Copyright: © 2023 American Chemical Society.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

metabolites
urinary excretion
UHPLC-HRMS analysis
mango polyphenols
method validation

Access to Document

10.1021/acs.jafc.3c03846

Caceres-Jimenez et al_Accepted versionAuthor Accepted Manuscript, 846 KB

Cite this

Cáceres-Jiménez, S., Rodríguez-Solana, R., Dobani, S., Pourshahidi, K., Gill, C., Moreno-Rojas, J. M., Almutairi, T. M., Crozier, A., & Pereira-Caro, G. (2023). UHPLC-HRMS Spectrometric Analysis: Method Validation and Plasma and Urinary Metabolite Identification after Mango Pulp Intake. Journal of Agricultural and Food Chemistry, 71(30), 11520-11533. https://doi.org/10.1021/acs.jafc.3c03846

@article{c86465884adf4ba18f46dc0a2ceedd0e,

title = "UHPLC-HRMS Spectrometric Analysis: Method Validation and Plasma and Urinary Metabolite Identification after Mango Pulp Intake",

abstract = "After an acute intake of 300 g of mango pur{\'e}e by 10 subjects, 0 and 24 h urine and plasma samples were analyzed by high-performance liquid chromatography–high-resolution mass spectrometry. The method was first validated for 44 reference polyphenols in terms of linearity, specificity, limits of detection and quantification, intra-day and inter-day precision, recovery, and matrix effects in two biological matrices. After method validation, a total of 94 microbial-derived phenolic catabolites, including 15 cinnamic acids, 3 phenylhydracrylic acids, 14 phenylpropanoic acids, 12 phenylacetic acids, 28 benzoic acids, 2 mandelic acids, 15 hydroxybenzenes, and 5 hippuric acid derivatives, were identified or tentatively identified in urine and/or plasma. These results establish the value of the UHPLC-HRMS protocol and the use of authentic standards to obtain a detailed and accurate picture of mango polyphenol metabolites, together with their phase II conjugated metabolites, in human bioavailability studies.",

keywords = "metabolites, urinary excretion, UHPLC-HRMS analysis, mango polyphenols, method validation",

author = "Salud C{\'a}ceres-Jim{\'e}nez and Raquel Rodr{\'i}guez-Solana and Sara Dobani and Kirsty Pourshahidi and Chris Gill and Moreno-Rojas, \{Jos{\'e} Manuel\} and Almutairi, \{Tahani M.\} and Alan Crozier and Gema Pereira-Caro",

note = "Funding Information: This study was supported by the project “Bioavailability of mango polyphenols in healthy and ileostomy patients: relevance of the bioactive forms in vivo on the gastrointestinal health” (RTI2018-096703-J-I00), funded by the European Regional Development Funds (ERDF), the Spanish Ministry of Science, Innovation and Universities (MCIU), and the Spanish State Research Agency (AEI). S.C.-J. was supported by a predoctoral fellowship funded by the Spanish Ministry of Universities (FPU20/03549). R.R.S. was supported by a Juan de la Cierva–Incorporation contract from the Spanish Ministry of Science, Innovation and Universities (IJC2018-036207-I). A.C. and T.M.A. were supported by the Distinguished Scientist Fellowship Program (DSFP) at King Saud University, Riyadh, Saudi Arabia. Publisher Copyright: {\textcopyright} 2023 American Chemical Society.",

year = "2023",

month = aug,

day = "2",

doi = "10.1021/acs.jafc.3c03846",

language = "English",

volume = "71",

pages = "11520--11533",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "30",

}

Cáceres-Jiménez, S, Rodríguez-Solana, R, Dobani, S, Pourshahidi, K , Gill, C, Moreno-Rojas, JM, Almutairi, TM, Crozier, A & Pereira-Caro, G 2023, 'UHPLC-HRMS Spectrometric Analysis: Method Validation and Plasma and Urinary Metabolite Identification after Mango Pulp Intake', Journal of Agricultural and Food Chemistry, vol. 71, no. 30, pp. 11520-11533. https://doi.org/10.1021/acs.jafc.3c03846

TY - JOUR

T1 - UHPLC-HRMS Spectrometric Analysis: Method Validation and Plasma and Urinary Metabolite Identification after Mango Pulp Intake

AU - Cáceres-Jiménez, Salud

AU - Rodríguez-Solana, Raquel

AU - Dobani, Sara

AU - Pourshahidi, Kirsty

AU - Gill, Chris

AU - Moreno-Rojas, José Manuel

AU - Almutairi, Tahani M.

AU - Crozier, Alan

AU - Pereira-Caro, Gema

N1 - Funding Information: This study was supported by the project “Bioavailability of mango polyphenols in healthy and ileostomy patients: relevance of the bioactive forms in vivo on the gastrointestinal health” (RTI2018-096703-J-I00), funded by the European Regional Development Funds (ERDF), the Spanish Ministry of Science, Innovation and Universities (MCIU), and the Spanish State Research Agency (AEI). S.C.-J. was supported by a predoctoral fellowship funded by the Spanish Ministry of Universities (FPU20/03549). R.R.S. was supported by a Juan de la Cierva–Incorporation contract from the Spanish Ministry of Science, Innovation and Universities (IJC2018-036207-I). A.C. and T.M.A. were supported by the Distinguished Scientist Fellowship Program (DSFP) at King Saud University, Riyadh, Saudi Arabia. Publisher Copyright: © 2023 American Chemical Society.

PY - 2023/8/2

Y1 - 2023/8/2

N2 - After an acute intake of 300 g of mango purée by 10 subjects, 0 and 24 h urine and plasma samples were analyzed by high-performance liquid chromatography–high-resolution mass spectrometry. The method was first validated for 44 reference polyphenols in terms of linearity, specificity, limits of detection and quantification, intra-day and inter-day precision, recovery, and matrix effects in two biological matrices. After method validation, a total of 94 microbial-derived phenolic catabolites, including 15 cinnamic acids, 3 phenylhydracrylic acids, 14 phenylpropanoic acids, 12 phenylacetic acids, 28 benzoic acids, 2 mandelic acids, 15 hydroxybenzenes, and 5 hippuric acid derivatives, were identified or tentatively identified in urine and/or plasma. These results establish the value of the UHPLC-HRMS protocol and the use of authentic standards to obtain a detailed and accurate picture of mango polyphenol metabolites, together with their phase II conjugated metabolites, in human bioavailability studies.

AB - After an acute intake of 300 g of mango purée by 10 subjects, 0 and 24 h urine and plasma samples were analyzed by high-performance liquid chromatography–high-resolution mass spectrometry. The method was first validated for 44 reference polyphenols in terms of linearity, specificity, limits of detection and quantification, intra-day and inter-day precision, recovery, and matrix effects in two biological matrices. After method validation, a total of 94 microbial-derived phenolic catabolites, including 15 cinnamic acids, 3 phenylhydracrylic acids, 14 phenylpropanoic acids, 12 phenylacetic acids, 28 benzoic acids, 2 mandelic acids, 15 hydroxybenzenes, and 5 hippuric acid derivatives, were identified or tentatively identified in urine and/or plasma. These results establish the value of the UHPLC-HRMS protocol and the use of authentic standards to obtain a detailed and accurate picture of mango polyphenol metabolites, together with their phase II conjugated metabolites, in human bioavailability studies.

KW - metabolites

KW - urinary excretion

KW - UHPLC-HRMS analysis

KW - mango polyphenols

KW - method validation

UR - https://www.scopus.com/pages/publications/85166389592

U2 - 10.1021/acs.jafc.3c03846

DO - 10.1021/acs.jafc.3c03846

M3 - Article

C2 - 37471325

SN - 0021-8561

VL - 71

SP - 11520

EP - 11533

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 30

ER -

UHPLC-HRMS Spectrometric Analysis: Method Validation and Plasma and Urinary Metabolite Identification after Mango Pulp Intake

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

VALGUT: phenyl-ϒ-valerolactones are microbiota-derived compounds that can impact gut health

Cite this

UHPLC-HRMS Spectrometric Analysis: Method Validation and Plasma and Urinary Metabolite Identification after Mango Pulp Intake

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Student theses

VALGUT: phenyl-ϒ-valerolactones are microbiota-derived compounds that can impact gut health

Cite this