UHPLC-HRMS Spectrometric Analysis: Method Validation and Plasma and Urinary Metabolite Identification after Mango Pulp Intake

Salud Cáceres-Jiménez, Raquel Rodríguez-Solana, Sara Dobani, Kirsty Pourshahidi, Chris Gill, José Manuel Moreno-Rojas, Tahani M. Almutairi, Alan Crozier, Gema Pereira-Caro

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

After an acute intake of 300 g of mango purée by 10 subjects, 0 and 24 h urine and plasma samples were analyzed by high-performance liquid chromatography–high-resolution mass spectrometry. The method was first validated for 44 reference polyphenols in terms of linearity, specificity, limits of detection and quantification, intra-day and inter-day precision, recovery, and matrix effects in two biological matrices. After method validation, a total of 94 microbial-derived phenolic catabolites, including 15 cinnamic acids, 3 phenylhydracrylic acids, 14 phenylpropanoic acids, 12 phenylacetic acids, 28 benzoic acids, 2 mandelic acids, 15 hydroxybenzenes, and 5 hippuric acid derivatives, were identified or tentatively identified in urine and/or plasma. These results establish the value of the UHPLC-HRMS protocol and the use of authentic standards to obtain a detailed and accurate picture of mango polyphenol metabolites, together with their phase II conjugated metabolites, in human bioavailability studies.
Original languageEnglish
Pages (from-to)11520-11533
Number of pages14
JournalJournal of Agricultural and Food Chemistry
Volume71
Issue number30
Early online date20 Jul 2023
DOIs
Publication statusPublished (in print/issue) - 2 Aug 2023

Bibliographical note

Funding Information:
This study was supported by the project “Bioavailability of mango polyphenols in healthy and ileostomy patients: relevance of the bioactive forms in vivo on the gastrointestinal health” (RTI2018-096703-J-I00), funded by the European Regional Development Funds (ERDF), the Spanish Ministry of Science, Innovation and Universities (MCIU), and the Spanish State Research Agency (AEI). S.C.-J. was supported by a predoctoral fellowship funded by the Spanish Ministry of Universities (FPU20/03549). R.R.S. was supported by a Juan de la Cierva–Incorporation contract from the Spanish Ministry of Science, Innovation and Universities (IJC2018-036207-I). A.C. and T.M.A. were supported by the Distinguished Scientist Fellowship Program (DSFP) at King Saud University, Riyadh, Saudi Arabia.

Publisher Copyright:
© 2023 American Chemical Society.

Keywords

  • metabolites
  • urinary excretion
  • UHPLC-HRMS analysis
  • mango polyphenols
  • method validation

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