Transfer of antibiotic resistance genes between Enterococcus faecalis strains in filter feeding zooplankton Daphnia magna and Daphnia pulex

Temilola Olukayode Olanrewaju, Mary McCarron, J Dooley, Joerg Arnscheidt

Research output: Contribution to journalArticle

Abstract

Antibiotic resistant bacteria from faecal pollution sources are pervasive in aquatic environments. A facilitating role for the emergence of waterborne, multi-drug resistant bacterial pathogens has been attributed to biofiltration but had not yet been substantiated. This study investigated the effect of filtration and gut passage in Daphnia spp. on conjugal transfer of resistance genes in Enterococcus faecalis. In vivo conjugation experiments involved a vancomycin-resistant donor strain bearing a plasmid-borne vanA resistance gene, and two vancomycin-susceptible and rifampicin-resistant recipient strains in the presence of Daphnia magna or Daphnia pulex. Results showed successful transfer of the vanA resistance gene from donor to recipient; gene identity was confirmed by PCR and DNA sequencing. There was no significant difference in the number of transconjugants recovered from D. magna and D. pulex. However, transconjugant numbers differed by one order of magnitude between recipient strains. Transconjugant numbers from D. magna were also significantly different between treatments with ingestion of individual phytoplankton species before filtration of bacteria. The highest transfer efficiency calculated from excreted transconjugants was 2.5 × 10-6. This proof of concept for facilitation of horizontal gene transfer by a filter feeding organism provides evidence that Daphnia can disseminate antibiotic resistant transconjugants in the environment.
LanguageEnglish
Pages1168-1175
Number of pages8
JournalScience of the Total Environment
Volume659
Early online date22 Dec 2018
DOIs
Publication statusPublished - 1 Apr 2019

Fingerprint

Zooplankton
Daphnia
Daphnia pulex
filter feeding
Daphnia magna
antibiotic resistance
Enterococcus faecalis
Antibiotics
Microbial Drug Resistance
zooplankton
Genes
Anti-Bacterial Agents
vancomycin
gene
Vancomycin
antibiotics
Bacteria
Bearings (structural)
genes
biofiltration

Keywords

  • Daphnia, antimicrobial resistance, vanA gene, conjugation, bacteria, Enterococcus faecalis, enterococci, Enterococcus, filter feeder, zooplankton, antibiotic resistance, horizontal transfer, multi-drug resistance, super bug, host microbe interaction, microbe, cladocera, palmellopsis, desmodesmus, chlorophyta
  • Enterococcus faecalis
  • Daphnia
  • Bacteria
  • Antimicrobial resistance
  • Conjugation
  • vanA gene

Cite this

@article{c2b6fab2d7a44c1aa0bf3f977f617727,
title = "Transfer of antibiotic resistance genes between Enterococcus faecalis strains in filter feeding zooplankton Daphnia magna and Daphnia pulex",
abstract = "Antibiotic resistant bacteria from faecal pollution sources are pervasive in aquatic environments. A facilitating role for the emergence of waterborne, multi-drug resistant bacterial pathogens has been attributed to biofiltration but had not yet been substantiated. This study investigated the effect of filtration and gut passage in Daphnia spp. on conjugal transfer of resistance genes in Enterococcus faecalis. In vivo conjugation experiments involved a vancomycin-resistant donor strain bearing a plasmid-borne vanA resistance gene, and two vancomycin-susceptible and rifampicin-resistant recipient strains in the presence of Daphnia magna or Daphnia pulex. Results showed successful transfer of the vanA resistance gene from donor to recipient; gene identity was confirmed by PCR and DNA sequencing. There was no significant difference in the number of transconjugants recovered from D. magna and D. pulex. However, transconjugant numbers differed by one order of magnitude between recipient strains. Transconjugant numbers from D. magna were also significantly different between treatments with ingestion of individual phytoplankton species before filtration of bacteria. The highest transfer efficiency calculated from excreted transconjugants was 2.5 × 10-6. This proof of concept for facilitation of horizontal gene transfer by a filter feeding organism provides evidence that Daphnia can disseminate antibiotic resistant transconjugants in the environment.",
keywords = "Daphnia, antimicrobial resistance, vanA gene, conjugation, bacteria, Enterococcus faecalis, enterococci, Enterococcus, filter feeder, zooplankton, antibiotic resistance, horizontal transfer, multi-drug resistance, super bug, host microbe interaction, microbe, cladocera, palmellopsis, desmodesmus, chlorophyta , Enterococcus faecalis, Daphnia, Bacteria, Antimicrobial resistance, Conjugation, vanA gene",
author = "Olanrewaju, {Temilola Olukayode} and Mary McCarron and J Dooley and Joerg Arnscheidt",
year = "2019",
month = "4",
day = "1",
doi = "10.1016/j.scitotenv.2018.12.314",
language = "English",
volume = "659",
pages = "1168--1175",

}

Transfer of antibiotic resistance genes between Enterococcus faecalis strains in filter feeding zooplankton Daphnia magna and Daphnia pulex. / Olanrewaju, Temilola Olukayode; McCarron, Mary; Dooley, J; Arnscheidt, Joerg.

Vol. 659, 01.04.2019, p. 1168-1175.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Transfer of antibiotic resistance genes between Enterococcus faecalis strains in filter feeding zooplankton Daphnia magna and Daphnia pulex

AU - Olanrewaju, Temilola Olukayode

AU - McCarron, Mary

AU - Dooley, J

AU - Arnscheidt, Joerg

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Antibiotic resistant bacteria from faecal pollution sources are pervasive in aquatic environments. A facilitating role for the emergence of waterborne, multi-drug resistant bacterial pathogens has been attributed to biofiltration but had not yet been substantiated. This study investigated the effect of filtration and gut passage in Daphnia spp. on conjugal transfer of resistance genes in Enterococcus faecalis. In vivo conjugation experiments involved a vancomycin-resistant donor strain bearing a plasmid-borne vanA resistance gene, and two vancomycin-susceptible and rifampicin-resistant recipient strains in the presence of Daphnia magna or Daphnia pulex. Results showed successful transfer of the vanA resistance gene from donor to recipient; gene identity was confirmed by PCR and DNA sequencing. There was no significant difference in the number of transconjugants recovered from D. magna and D. pulex. However, transconjugant numbers differed by one order of magnitude between recipient strains. Transconjugant numbers from D. magna were also significantly different between treatments with ingestion of individual phytoplankton species before filtration of bacteria. The highest transfer efficiency calculated from excreted transconjugants was 2.5 × 10-6. This proof of concept for facilitation of horizontal gene transfer by a filter feeding organism provides evidence that Daphnia can disseminate antibiotic resistant transconjugants in the environment.

AB - Antibiotic resistant bacteria from faecal pollution sources are pervasive in aquatic environments. A facilitating role for the emergence of waterborne, multi-drug resistant bacterial pathogens has been attributed to biofiltration but had not yet been substantiated. This study investigated the effect of filtration and gut passage in Daphnia spp. on conjugal transfer of resistance genes in Enterococcus faecalis. In vivo conjugation experiments involved a vancomycin-resistant donor strain bearing a plasmid-borne vanA resistance gene, and two vancomycin-susceptible and rifampicin-resistant recipient strains in the presence of Daphnia magna or Daphnia pulex. Results showed successful transfer of the vanA resistance gene from donor to recipient; gene identity was confirmed by PCR and DNA sequencing. There was no significant difference in the number of transconjugants recovered from D. magna and D. pulex. However, transconjugant numbers differed by one order of magnitude between recipient strains. Transconjugant numbers from D. magna were also significantly different between treatments with ingestion of individual phytoplankton species before filtration of bacteria. The highest transfer efficiency calculated from excreted transconjugants was 2.5 × 10-6. This proof of concept for facilitation of horizontal gene transfer by a filter feeding organism provides evidence that Daphnia can disseminate antibiotic resistant transconjugants in the environment.

KW - Daphnia, antimicrobial resistance, vanA gene, conjugation, bacteria, Enterococcus faecalis, enterococci, Enterococcus, filter feeder, zooplankton, antibiotic resistance, horizontal transfer, multi-drug resistance, super bug, host microbe interaction, micr

KW - Enterococcus faecalis

KW - Daphnia

KW - Bacteria

KW - Antimicrobial resistance

KW - Conjugation

KW - vanA gene

UR - http://www.scopus.com/inward/record.url?scp=85059515314&partnerID=8YFLogxK

U2 - 10.1016/j.scitotenv.2018.12.314

DO - 10.1016/j.scitotenv.2018.12.314

M3 - Article

VL - 659

SP - 1168

EP - 1175

ER -