Towards efficient crude oil degradation by a mixed bacterial consortium

KSM Rahman, J Thahira-Rahman, P Lakshmanaperumalsamy, Ibrahim Banat

Research output: Contribution to journalArticle

237 Citations (Scopus)

Abstract

A laboratory study was undertaken to assess the optimal conditions for biodegradation of Bombay High (BH) crude oil. Among 130 oil degrading bacterial cultures isolated from oil contaminated soil samples, Micrococcus sp. GS2-22, Corynebacterium sp. GS5-66, Flavobacterium sp. DS5-73, Bacillus sp. DS6-86 and Pseudomonas sp. DS10-129 were selected for the study based on the efficiency of crude oil utilisation. A mixed bacterial consortium prepared using the above strains was also used. Individual bacterial cultures showed less growth and degradation than did the mixed bacterial consortium. At 1% crude oil concentration, the mixed bacterial consortium degraded a maximum of 78% of BH crude oil. This was followed by 66% by Pseudomonas sp. DS10-129, 59% by Bacillus sp. DS6-86, 49% by Micrococcus sp. GS2-22, 43% by Corynebacterium sp. GS5-66 and 41% by Flavobacterium sp. DS5-73. The percentage of degradation by the mixed bacterial consortium decreased from 78% to 52% as the concentration of crude oil was increased from 1% to 10%. Temperature of 30 degreesC and pH 7.5 were found to be optima for maximum biodegradation. (C) 2002 Elsevier Science Ltd. All rights reserved.
LanguageEnglish
Pages257-261
JournalBioresource Technology
Volume85
Issue number3
Publication statusPublished - Dec 2002

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crude oil
degradation
biodegradation
oil
temperature

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Rahman, KSM., Thahira-Rahman, J., Lakshmanaperumalsamy, P., & Banat, I. (2002). Towards efficient crude oil degradation by a mixed bacterial consortium. 85(3), 257-261.
Rahman, KSM ; Thahira-Rahman, J ; Lakshmanaperumalsamy, P ; Banat, Ibrahim. / Towards efficient crude oil degradation by a mixed bacterial consortium. 2002 ; Vol. 85, No. 3. pp. 257-261.
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Rahman, KSM, Thahira-Rahman, J, Lakshmanaperumalsamy, P & Banat, I 2002, 'Towards efficient crude oil degradation by a mixed bacterial consortium', vol. 85, no. 3, pp. 257-261.

Towards efficient crude oil degradation by a mixed bacterial consortium. / Rahman, KSM; Thahira-Rahman, J; Lakshmanaperumalsamy, P; Banat, Ibrahim.

Vol. 85, No. 3, 12.2002, p. 257-261.

Research output: Contribution to journalArticle

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Rahman KSM, Thahira-Rahman J, Lakshmanaperumalsamy P, Banat I. Towards efficient crude oil degradation by a mixed bacterial consortium. 2002 Dec;85(3):257-261.