Towards a microfluidic enzyme assay system

R Holmes

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    This presentation describes work into a prototype system for the assay of amylase, using microfludic technologies. The new system has a significantly shorter cycle time than the current laboratory methods, which generally use microtitre plates, yet is capable of generating significantly superior results. As such, we have shown that sensitivity is enhanced by a factor of 10 in the initial trials, whilst reducing the reaction time by a factor of 6.2, from 20 minutes incubation to 3.2 minutes. Basing the conclusion on the Megazyme Cerealpha Standard Method, and using the Cerealpha Units as a measure of assay efficiency, The typical response for the microfluidic assay was shown to be 1.0x10-3 CU/ml (SD 2.5x10-4 U/ml), compared to 2.56x10-4 CU/ml (SD 5.94x10-5 CU/ml) for the standard macro-assay. It is believed that this improvement in the reaction schematics is due to the inherent advantages of microfluidic devices such as superior mixing, higher thermal efficiency and enhanced reaction kinetics.
    LanguageEnglish
    Title of host publicationUnknown Host Publication
    Number of pages14
    Publication statusPublished - 3 Oct 2008
    EventMANCHESTER INTERDISCIPLINARY BIOSCIENCE CENTRE - RESEARCH CONFERENCE 2008 - MANCHESTER INTERDISCIPLINARY BIOSCIENCE CENTRE
    Duration: 3 Oct 2008 → …

    Conference

    ConferenceMANCHESTER INTERDISCIPLINARY BIOSCIENCE CENTRE - RESEARCH CONFERENCE 2008
    Period3/10/08 → …

    Fingerprint

    Microfluidics
    Assays
    Enzymes
    Schematic diagrams
    Amylases
    Reaction kinetics
    Macros

    Cite this

    Holmes, R. (2008). Towards a microfluidic enzyme assay system. In Unknown Host Publication
    Holmes, R. / Towards a microfluidic enzyme assay system. Unknown Host Publication. 2008.
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    abstract = "This presentation describes work into a prototype system for the assay of amylase, using microfludic technologies. The new system has a significantly shorter cycle time than the current laboratory methods, which generally use microtitre plates, yet is capable of generating significantly superior results. As such, we have shown that sensitivity is enhanced by a factor of 10 in the initial trials, whilst reducing the reaction time by a factor of 6.2, from 20 minutes incubation to 3.2 minutes. Basing the conclusion on the Megazyme Cerealpha Standard Method, and using the Cerealpha Units as a measure of assay efficiency, The typical response for the microfluidic assay was shown to be 1.0x10-3 CU/ml (SD 2.5x10-4 U/ml), compared to 2.56x10-4 CU/ml (SD 5.94x10-5 CU/ml) for the standard macro-assay. It is believed that this improvement in the reaction schematics is due to the inherent advantages of microfluidic devices such as superior mixing, higher thermal efficiency and enhanced reaction kinetics.",
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    Holmes, R 2008, Towards a microfluidic enzyme assay system. in Unknown Host Publication. MANCHESTER INTERDISCIPLINARY BIOSCIENCE CENTRE - RESEARCH CONFERENCE 2008, 3/10/08.

    Towards a microfluidic enzyme assay system. / Holmes, R.

    Unknown Host Publication. 2008.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

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    T1 - Towards a microfluidic enzyme assay system

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    AB - This presentation describes work into a prototype system for the assay of amylase, using microfludic technologies. The new system has a significantly shorter cycle time than the current laboratory methods, which generally use microtitre plates, yet is capable of generating significantly superior results. As such, we have shown that sensitivity is enhanced by a factor of 10 in the initial trials, whilst reducing the reaction time by a factor of 6.2, from 20 minutes incubation to 3.2 minutes. Basing the conclusion on the Megazyme Cerealpha Standard Method, and using the Cerealpha Units as a measure of assay efficiency, The typical response for the microfluidic assay was shown to be 1.0x10-3 CU/ml (SD 2.5x10-4 U/ml), compared to 2.56x10-4 CU/ml (SD 5.94x10-5 CU/ml) for the standard macro-assay. It is believed that this improvement in the reaction schematics is due to the inherent advantages of microfluidic devices such as superior mixing, higher thermal efficiency and enhanced reaction kinetics.

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    Holmes R. Towards a microfluidic enzyme assay system. In Unknown Host Publication. 2008