Towards a microfluidic based rapid amylase assay system

RJ Holmes, P Summesgil, T Ryan, BJ Treve Brown, A Mockbil, BD Grieve, PR Fielden

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    This article describes work into a prototype system for the assay of amylase, using microfludic technologies. The new system has a significantly shorter cycle time than the current laboratory methods, which generally use microtitre plates, yet is capable of generating significantly superior results. As such, we have shown that sensitivity is enhanced by a factor of 10 in the standard assay trials, and by a factor of 2 in the real-sample lab trials. In both assays, the use of a microreactor system reduced the reaction time by a factor of 6.2, from 20 min incubation to 3.2 min. Basing the conclusion on the Megazyme Cerealpha Standard Method, and using the Cerealpha units as a measure of assay efficiency, the typical response for the microfluidic assay was shown to be 1.0 × 10−3 CU/mL (standard deviation [SD] 2.5 × 10−4 CU/mL), compared to 2.56 × 10−4 CU/mL (SD 5.94 × 10−5 CU/mL) for the standard macroassay. It is believed that this improvement in the reaction schematics is due to the inherent advantages of microfluidic devices such as superior mixing, higher thermal efficiency, and enhanced reaction kinetics.
    LanguageEnglish
    PagesN37-N43
    JournalJournal of Food Science
    Volume74
    Issue number6
    DOIs
    Publication statusPublished - 2009

    Fingerprint

    Amylases
    Microfluidics
    Assays
    Schematic diagrams
    Reaction kinetics

    Keywords

    • amylase
    • analysis
    • enzyme assay
    • instrumentation
    • microfluidics

    Cite this

    Holmes, RJ., Summesgil, P., Ryan, T., Treve Brown, BJ., Mockbil, A., Grieve, BD., & Fielden, PR. (2009). Towards a microfluidic based rapid amylase assay system. Journal of Food Science, 74(6), N37-N43. https://doi.org/10.1111/j.1750-3841.2009.01235.x
    Holmes, RJ ; Summesgil, P ; Ryan, T ; Treve Brown, BJ ; Mockbil, A ; Grieve, BD ; Fielden, PR. / Towards a microfluidic based rapid amylase assay system. In: Journal of Food Science. 2009 ; Vol. 74, No. 6. pp. N37-N43.
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    Holmes, RJ, Summesgil, P, Ryan, T, Treve Brown, BJ, Mockbil, A, Grieve, BD & Fielden, PR 2009, 'Towards a microfluidic based rapid amylase assay system', Journal of Food Science, vol. 74, no. 6, pp. N37-N43. https://doi.org/10.1111/j.1750-3841.2009.01235.x

    Towards a microfluidic based rapid amylase assay system. / Holmes, RJ; Summesgil, P; Ryan, T; Treve Brown, BJ; Mockbil, A; Grieve, BD; Fielden, PR.

    In: Journal of Food Science, Vol. 74, No. 6, 2009, p. N37-N43.

    Research output: Contribution to journalArticle

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    AU - Grieve, BD

    AU - Fielden, PR

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    AB - This article describes work into a prototype system for the assay of amylase, using microfludic technologies. The new system has a significantly shorter cycle time than the current laboratory methods, which generally use microtitre plates, yet is capable of generating significantly superior results. As such, we have shown that sensitivity is enhanced by a factor of 10 in the standard assay trials, and by a factor of 2 in the real-sample lab trials. In both assays, the use of a microreactor system reduced the reaction time by a factor of 6.2, from 20 min incubation to 3.2 min. Basing the conclusion on the Megazyme Cerealpha Standard Method, and using the Cerealpha units as a measure of assay efficiency, the typical response for the microfluidic assay was shown to be 1.0 × 10−3 CU/mL (standard deviation [SD] 2.5 × 10−4 CU/mL), compared to 2.56 × 10−4 CU/mL (SD 5.94 × 10−5 CU/mL) for the standard macroassay. It is believed that this improvement in the reaction schematics is due to the inherent advantages of microfluidic devices such as superior mixing, higher thermal efficiency, and enhanced reaction kinetics.

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    Holmes RJ, Summesgil P, Ryan T, Treve Brown BJ, Mockbil A, Grieve BD et al. Towards a microfluidic based rapid amylase assay system. Journal of Food Science. 2009;74(6):N37-N43. https://doi.org/10.1111/j.1750-3841.2009.01235.x