Total radical-trapping antioxidant potential (TRAP) of plasma effects of supplementaiton of young healthy volunteers with large doses of alpha-tocopherol and ascorbic-acid

CW Mulholland, JJ Strain

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Abstract

A randomized, placebo-controlled double-blind supplementation trial was conducted on 32 adults to assess the effect of dl-alpha-tocopherol (1 g/day for 28 days) or ascorbic acid (AA) (1 g/day for 28 days) separately or in combination on the total radical-trapping antioxidant potential (TRAP) of plasma. Subjects were divided into 4 groups of 8 and received the following: Group 1, placebo, Group 2, ascorbic acid; Group 3, alpha-tocopherol; Group 4, alpha-tocopherol and AA. Antioxidant potential was assessed using the TRAP technique (a specific measure of the capacity of the secondary antioxidants, vitamins E, C, reduced thiol compounds and urate in plasma to resist controlled in vitro peroxidation). TRAP values were estimated at baseline and after treatment (day 29). Group means of the antioxidant vitamins at baseline were within normal values for both ascorbic acid (62-82 mumol/l) and alpha-tocopherol (24-29 mumol/l). Despite marked increases post-supplementation in plasma alpha-tocopherol levels in Groups 3 (94%) and 4 (91%) and in AA levels in Groups 2 (82%) and 4 (32%), there were no significant differences (analysis of variance) between the 4 treatment groups for TRAP values on day 29. Paired-t-testing revealed significant (P < 0.01) increases in postsupplementation TRAP values in Groups 2 and 3.
LanguageEnglish
Pages27-30
JournalInternational Journal for Vitamin and Nutrition Research
Volume63
Issue number1
Publication statusPublished - 1993

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alpha-tocopherol
volunteers
trapping
ascorbic acid
antioxidants
dosage
placebos
peroxidation
thiols
normal values
vitamin E
vitamins
analysis of variance

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@article{67518bf9717549e4aa05b85f9ff974aa,
title = "Total radical-trapping antioxidant potential (TRAP) of plasma effects of supplementaiton of young healthy volunteers with large doses of alpha-tocopherol and ascorbic-acid",
abstract = "A randomized, placebo-controlled double-blind supplementation trial was conducted on 32 adults to assess the effect of dl-alpha-tocopherol (1 g/day for 28 days) or ascorbic acid (AA) (1 g/day for 28 days) separately or in combination on the total radical-trapping antioxidant potential (TRAP) of plasma. Subjects were divided into 4 groups of 8 and received the following: Group 1, placebo, Group 2, ascorbic acid; Group 3, alpha-tocopherol; Group 4, alpha-tocopherol and AA. Antioxidant potential was assessed using the TRAP technique (a specific measure of the capacity of the secondary antioxidants, vitamins E, C, reduced thiol compounds and urate in plasma to resist controlled in vitro peroxidation). TRAP values were estimated at baseline and after treatment (day 29). Group means of the antioxidant vitamins at baseline were within normal values for both ascorbic acid (62-82 mumol/l) and alpha-tocopherol (24-29 mumol/l). Despite marked increases post-supplementation in plasma alpha-tocopherol levels in Groups 3 (94{\%}) and 4 (91{\%}) and in AA levels in Groups 2 (82{\%}) and 4 (32{\%}), there were no significant differences (analysis of variance) between the 4 treatment groups for TRAP values on day 29. Paired-t-testing revealed significant (P < 0.01) increases in postsupplementation TRAP values in Groups 2 and 3.",
author = "CW Mulholland and JJ Strain",
year = "1993",
language = "English",
volume = "63",
pages = "27--30",
journal = "International Journal for Vitamin and Nutrition Research",
issn = "0300-9831",
publisher = "Hogrefe Publishing",
number = "1",

}

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T1 - Total radical-trapping antioxidant potential (TRAP) of plasma effects of supplementaiton of young healthy volunteers with large doses of alpha-tocopherol and ascorbic-acid

AU - Mulholland, CW

AU - Strain, JJ

PY - 1993

Y1 - 1993

N2 - A randomized, placebo-controlled double-blind supplementation trial was conducted on 32 adults to assess the effect of dl-alpha-tocopherol (1 g/day for 28 days) or ascorbic acid (AA) (1 g/day for 28 days) separately or in combination on the total radical-trapping antioxidant potential (TRAP) of plasma. Subjects were divided into 4 groups of 8 and received the following: Group 1, placebo, Group 2, ascorbic acid; Group 3, alpha-tocopherol; Group 4, alpha-tocopherol and AA. Antioxidant potential was assessed using the TRAP technique (a specific measure of the capacity of the secondary antioxidants, vitamins E, C, reduced thiol compounds and urate in plasma to resist controlled in vitro peroxidation). TRAP values were estimated at baseline and after treatment (day 29). Group means of the antioxidant vitamins at baseline were within normal values for both ascorbic acid (62-82 mumol/l) and alpha-tocopherol (24-29 mumol/l). Despite marked increases post-supplementation in plasma alpha-tocopherol levels in Groups 3 (94%) and 4 (91%) and in AA levels in Groups 2 (82%) and 4 (32%), there were no significant differences (analysis of variance) between the 4 treatment groups for TRAP values on day 29. Paired-t-testing revealed significant (P < 0.01) increases in postsupplementation TRAP values in Groups 2 and 3.

AB - A randomized, placebo-controlled double-blind supplementation trial was conducted on 32 adults to assess the effect of dl-alpha-tocopherol (1 g/day for 28 days) or ascorbic acid (AA) (1 g/day for 28 days) separately or in combination on the total radical-trapping antioxidant potential (TRAP) of plasma. Subjects were divided into 4 groups of 8 and received the following: Group 1, placebo, Group 2, ascorbic acid; Group 3, alpha-tocopherol; Group 4, alpha-tocopherol and AA. Antioxidant potential was assessed using the TRAP technique (a specific measure of the capacity of the secondary antioxidants, vitamins E, C, reduced thiol compounds and urate in plasma to resist controlled in vitro peroxidation). TRAP values were estimated at baseline and after treatment (day 29). Group means of the antioxidant vitamins at baseline were within normal values for both ascorbic acid (62-82 mumol/l) and alpha-tocopherol (24-29 mumol/l). Despite marked increases post-supplementation in plasma alpha-tocopherol levels in Groups 3 (94%) and 4 (91%) and in AA levels in Groups 2 (82%) and 4 (32%), there were no significant differences (analysis of variance) between the 4 treatment groups for TRAP values on day 29. Paired-t-testing revealed significant (P < 0.01) increases in postsupplementation TRAP values in Groups 2 and 3.

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