Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Chemical Constituents

Graeme Kirkpatrick, P Nigam, R.K. Owusu-Apenten

Research output: Contribution to journalArticle

Abstract

Aims: To compare the total phenol content, antioxidant capacity and antibacterial activity of methyl syringate (MSY), methylglyoxal (MGO) and phenyllactic acid (PLA) as major components from Manuka honeyStudy Design: In-vitro study.Place and Duration of Study: Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, University of Ulster, Coleraine Campus, between June 2016 and September 2017.Methodology: Total phenols content (TPC) was determined using the Folin-Ciocalteu assay. Antioxidant capacity was evaluated as 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical quenching activity or iron (III) reducing antioxidant capacity (IRAC). Antibacterial activity was measured using the disc diffusion assay with, E.coli, Bacillus subtilis or Staphylococcus aureus.Results: The TPC for MSY was 60.8 % gallic acid equivalents (GAE) and significantly higher than 1.6-3.2% GAE observed for MGO or PLA. The antioxidant capacity for MSY (128% to 270% trolox equivalents (TE)) was significantly higher compared with -6% to 4.4%TE for MGO or PLA. A disc diffusion assay for MGO and PLA showed antibacterial power but MSY had no antibacterial activity. Conclusion: Methylglyoxal and PLA from Manuka honey showed antibacterial activity but no detectable antioxidant and total phenol character. Methyl syringate, which shows high antioxidant capacity and TPC, had no detectable antibacterial activity. Total phenols content and antioxidant power of Manuka honey is unlikely to be related to its antibacterial activity.
LanguageEnglish
Pages1-7
JournalJournal of Advances in Biology and Biotechnology
Volume15
Issue number4
DOIs
Publication statusPublished - 6 Nov 2017

Fingerprint

Honey
Phenols
Pyruvaldehyde
Antioxidants
Acids
Gallic Acid
Phenol
School Health Services
Sulfonic Acids
Bacillus subtilis
Staphylococcus aureus
Iron
methyl syringate
Escherichia coli
Food

Keywords

  • Manuka honey
  • honey components
  • antibacterial
  • antioxidant
  • methylglyoxal
  • methyl syringate
  • phenyllactic Acid

Cite this

Kirkpatrick, Graeme ; Nigam, P ; Owusu-Apenten, R.K. / Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Chemical Constituents. 2017 ; Vol. 15, No. 4. pp. 1-7.
@article{c3a257baf6154c5c8f19c20ced11c36a,
title = "Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Chemical Constituents",
abstract = "Aims: To compare the total phenol content, antioxidant capacity and antibacterial activity of methyl syringate (MSY), methylglyoxal (MGO) and phenyllactic acid (PLA) as major components from Manuka honeyStudy Design: In-vitro study.Place and Duration of Study: Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, University of Ulster, Coleraine Campus, between June 2016 and September 2017.Methodology: Total phenols content (TPC) was determined using the Folin-Ciocalteu assay. Antioxidant capacity was evaluated as 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical quenching activity or iron (III) reducing antioxidant capacity (IRAC). Antibacterial activity was measured using the disc diffusion assay with, E.coli, Bacillus subtilis or Staphylococcus aureus.Results: The TPC for MSY was 60.8 {\%} gallic acid equivalents (GAE) and significantly higher than 1.6-3.2{\%} GAE observed for MGO or PLA. The antioxidant capacity for MSY (128{\%} to 270{\%} trolox equivalents (TE)) was significantly higher compared with -6{\%} to 4.4{\%}TE for MGO or PLA. A disc diffusion assay for MGO and PLA showed antibacterial power but MSY had no antibacterial activity. Conclusion: Methylglyoxal and PLA from Manuka honey showed antibacterial activity but no detectable antioxidant and total phenol character. Methyl syringate, which shows high antioxidant capacity and TPC, had no detectable antibacterial activity. Total phenols content and antioxidant power of Manuka honey is unlikely to be related to its antibacterial activity.",
keywords = "Manuka honey, honey components, antibacterial, antioxidant, methylglyoxal, methyl syringate, phenyllactic Acid",
author = "Graeme Kirkpatrick and P Nigam and R.K. Owusu-Apenten",
year = "2017",
month = "11",
day = "6",
doi = "10.9734/JABB/2017/37242",
language = "English",
volume = "15",
pages = "1--7",
number = "4",

}

Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Chemical Constituents. / Kirkpatrick, Graeme; Nigam, P; Owusu-Apenten, R.K.

Vol. 15, No. 4, 06.11.2017, p. 1-7.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Chemical Constituents

AU - Kirkpatrick, Graeme

AU - Nigam, P

AU - Owusu-Apenten, R.K.

PY - 2017/11/6

Y1 - 2017/11/6

N2 - Aims: To compare the total phenol content, antioxidant capacity and antibacterial activity of methyl syringate (MSY), methylglyoxal (MGO) and phenyllactic acid (PLA) as major components from Manuka honeyStudy Design: In-vitro study.Place and Duration of Study: Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, University of Ulster, Coleraine Campus, between June 2016 and September 2017.Methodology: Total phenols content (TPC) was determined using the Folin-Ciocalteu assay. Antioxidant capacity was evaluated as 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical quenching activity or iron (III) reducing antioxidant capacity (IRAC). Antibacterial activity was measured using the disc diffusion assay with, E.coli, Bacillus subtilis or Staphylococcus aureus.Results: The TPC for MSY was 60.8 % gallic acid equivalents (GAE) and significantly higher than 1.6-3.2% GAE observed for MGO or PLA. The antioxidant capacity for MSY (128% to 270% trolox equivalents (TE)) was significantly higher compared with -6% to 4.4%TE for MGO or PLA. A disc diffusion assay for MGO and PLA showed antibacterial power but MSY had no antibacterial activity. Conclusion: Methylglyoxal and PLA from Manuka honey showed antibacterial activity but no detectable antioxidant and total phenol character. Methyl syringate, which shows high antioxidant capacity and TPC, had no detectable antibacterial activity. Total phenols content and antioxidant power of Manuka honey is unlikely to be related to its antibacterial activity.

AB - Aims: To compare the total phenol content, antioxidant capacity and antibacterial activity of methyl syringate (MSY), methylglyoxal (MGO) and phenyllactic acid (PLA) as major components from Manuka honeyStudy Design: In-vitro study.Place and Duration of Study: Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, University of Ulster, Coleraine Campus, between June 2016 and September 2017.Methodology: Total phenols content (TPC) was determined using the Folin-Ciocalteu assay. Antioxidant capacity was evaluated as 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical quenching activity or iron (III) reducing antioxidant capacity (IRAC). Antibacterial activity was measured using the disc diffusion assay with, E.coli, Bacillus subtilis or Staphylococcus aureus.Results: The TPC for MSY was 60.8 % gallic acid equivalents (GAE) and significantly higher than 1.6-3.2% GAE observed for MGO or PLA. The antioxidant capacity for MSY (128% to 270% trolox equivalents (TE)) was significantly higher compared with -6% to 4.4%TE for MGO or PLA. A disc diffusion assay for MGO and PLA showed antibacterial power but MSY had no antibacterial activity. Conclusion: Methylglyoxal and PLA from Manuka honey showed antibacterial activity but no detectable antioxidant and total phenol character. Methyl syringate, which shows high antioxidant capacity and TPC, had no detectable antibacterial activity. Total phenols content and antioxidant power of Manuka honey is unlikely to be related to its antibacterial activity.

KW - Manuka honey

KW - honey components

KW - antibacterial

KW - antioxidant

KW - methylglyoxal

KW - methyl syringate

KW - phenyllactic Acid

U2 - 10.9734/JABB/2017/37242

DO - 10.9734/JABB/2017/37242

M3 - Article

VL - 15

SP - 1

EP - 7

IS - 4

ER -