Topological Characterization of G-Quadruplexes by UV Absorption and Circular Dichroism

Ioannis Karsisiotis, N Ma’ani Hessari, E Novellino, GP Spada, A Randazzo, Mateus Webba da Silva

Research output: Contribution to journalArticle

223 Citations (Scopus)

Abstract

The emergence of nucleic acid four-stranded architectures, denominated G-quadruplexes as a prolific area of research, has led to an interest in the development of inexpensive methods for the rapid assessment of their structural characterization in solution. Research in this area is motivated by their potential impact in regulation of biological mechanisms and technological applications. For many applications, light absorption techniques, such as circular dichroism (CD) and UV, have been sufficient to discriminate the quadruplex fold from other architectures. CD is also useful to discriminate a single quadruplex topology from all other 25 generic folding topologies. Here we demonstrate the use of these techniques for characterizing three different types of G-quadruplex topologies classified through the sequence of glycosidic bond angles (GBA) adopted by guanosines of the G-quadruplex stem.
Original languageEnglish
Pages (from-to)10645-10648
JournalAngewandte Chemie International Edition
Volume50
DOIs
Publication statusPublished - Oct 2011

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