Top-down proteomic analysis of the soluble sub-proteome of the obligate thermophile, Geobacillus thermoleovorans T80: Insights into its cellular processes

RLJ Graham, CE Pollock, Nigel Ternan, Geoffrey McMullan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We report the first analysis of the soluble sub-proteome of the obligate thermophile, Geobacillus thermoleovorans T80, utilizing a robust multidimensional protein identification protocol. A total of 1336 proteins were initially identified utilizing automated MS/MS identification software. Intensive manual curation resulted in a final list containing a total of 294 unique proteins. Physiochemical characterization and functional classification of the soluble sub-proteome was carried out. The strategy has allowed us to gain an insight into the cellular processes of this obligate thermophile, identifying a variety of proteins known to play a role in stress response. Included within these were a number of sigma factors such as sigma(A) that initiate transcription of the heat shock operons controlled by the HrcA-CIRCE complex within gram positive bacteria. In addition, it has enabled us to assign a degree of functionality to 29 out of 36 gene products detected in this study that were hitherto described as being only hypothetical conserved proteins.
LanguageEnglish
Pages822-828
JournalJournal of Proteome Research
Volume5
Issue number4
DOIs
Publication statusPublished - Apr 2006

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Bacillus thermoleovorans
thermophilic microorganisms
proteome
proteomics
proteins
sigma factors
operon
Gram-positive bacteria
heat stress
stress response
transcription (genetics)
taxonomy

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abstract = "We report the first analysis of the soluble sub-proteome of the obligate thermophile, Geobacillus thermoleovorans T80, utilizing a robust multidimensional protein identification protocol. A total of 1336 proteins were initially identified utilizing automated MS/MS identification software. Intensive manual curation resulted in a final list containing a total of 294 unique proteins. Physiochemical characterization and functional classification of the soluble sub-proteome was carried out. The strategy has allowed us to gain an insight into the cellular processes of this obligate thermophile, identifying a variety of proteins known to play a role in stress response. Included within these were a number of sigma factors such as sigma(A) that initiate transcription of the heat shock operons controlled by the HrcA-CIRCE complex within gram positive bacteria. In addition, it has enabled us to assign a degree of functionality to 29 out of 36 gene products detected in this study that were hitherto described as being only hypothetical conserved proteins.",
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Top-down proteomic analysis of the soluble sub-proteome of the obligate thermophile, Geobacillus thermoleovorans T80: Insights into its cellular processes. / Graham, RLJ; Pollock, CE; Ternan, Nigel; McMullan, Geoffrey.

In: Journal of Proteome Research, Vol. 5, No. 4, 04.2006, p. 822-828.

Research output: Contribution to journalArticle

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