Toll-like receptor agonist induced changes in clonal rat BRIN-BD11 beta-cell insulin secretion and signal transduction.

A Kiely A, A Robinson A, Neville H. McClenaghan, Peter Flatt, P Newsholme P

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Evidence for involvement of toll-like receptors (TLRs) (e.g. TLR4 and TLR2, whose agonists include lipopolysaccharides (LPS) and saturated fatty acids) in altered patterns of signalling in adipose, liver and muscle from animal models of insulin resistance and obesity has been published. We have now extended this area of research and have determined the effects of LPS on cell viability, insulin secretion, insulin signalling and metabolism in a clonal beta-cell line. BRIN-BD11 beta-cells were treated for 24 h with increasing concentrations of LPS. Chronic (24 h) and acute (20 min) insulin secretion, insulin content and parameters of cell metabolism and insulin signalling were determined. Incubation of BRIN-BD11 cells for 24 h in the presence of increasing concentrations of the TLR4 ligand LPS significantly decreased chronic (24 h) insulin secretion from 1.09+/-0.19 to 0.76+/-0.18 microg insulin/mg protein in the presence of 100 ng/ml LPS (P
LanguageEnglish
Pages365-373
JournalJournal of Endrocrinology
Volume202
Issue number3
DOIs
Publication statusPublished - 24 Jun 2009

Fingerprint

Toll-Like Receptors
Signal Transduction
Insulin
Lipopolysaccharides
Insulin Resistance
Cell Survival
Fatty Acids
Animal Models
Obesity
Ligands
Cell Line
Muscles
Liver
Research
Proteins

Cite this

Kiely A, A ; Robinson A, A ; McClenaghan, Neville H. ; Flatt, Peter ; Newsholme P, P. / Toll-like receptor agonist induced changes in clonal rat BRIN-BD11 beta-cell insulin secretion and signal transduction. In: Journal of Endrocrinology. 2009 ; Vol. 202, No. 3. pp. 365-373.
@article{f79b1b63ed0a4b54a1b151e6b706aaa9,
title = "Toll-like receptor agonist induced changes in clonal rat BRIN-BD11 beta-cell insulin secretion and signal transduction.",
abstract = "Evidence for involvement of toll-like receptors (TLRs) (e.g. TLR4 and TLR2, whose agonists include lipopolysaccharides (LPS) and saturated fatty acids) in altered patterns of signalling in adipose, liver and muscle from animal models of insulin resistance and obesity has been published. We have now extended this area of research and have determined the effects of LPS on cell viability, insulin secretion, insulin signalling and metabolism in a clonal beta-cell line. BRIN-BD11 beta-cells were treated for 24 h with increasing concentrations of LPS. Chronic (24 h) and acute (20 min) insulin secretion, insulin content and parameters of cell metabolism and insulin signalling were determined. Incubation of BRIN-BD11 cells for 24 h in the presence of increasing concentrations of the TLR4 ligand LPS significantly decreased chronic (24 h) insulin secretion from 1.09+/-0.19 to 0.76+/-0.18 microg insulin/mg protein in the presence of 100 ng/ml LPS (P",
author = "{Kiely A}, A and {Robinson A}, A and McClenaghan, {Neville H.} and Peter Flatt and {Newsholme P}, P",
year = "2009",
month = "6",
day = "24",
doi = "10.1677/JOE-09-0160",
language = "English",
volume = "202",
pages = "365--373",
journal = "Journal of Endrocrinology",
issn = "0022-0795",
number = "3",

}

Toll-like receptor agonist induced changes in clonal rat BRIN-BD11 beta-cell insulin secretion and signal transduction. / Kiely A, A; Robinson A, A; McClenaghan, Neville H.; Flatt, Peter; Newsholme P, P.

In: Journal of Endrocrinology, Vol. 202, No. 3, 24.06.2009, p. 365-373.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Toll-like receptor agonist induced changes in clonal rat BRIN-BD11 beta-cell insulin secretion and signal transduction.

AU - Kiely A, A

AU - Robinson A, A

AU - McClenaghan, Neville H.

AU - Flatt, Peter

AU - Newsholme P, P

PY - 2009/6/24

Y1 - 2009/6/24

N2 - Evidence for involvement of toll-like receptors (TLRs) (e.g. TLR4 and TLR2, whose agonists include lipopolysaccharides (LPS) and saturated fatty acids) in altered patterns of signalling in adipose, liver and muscle from animal models of insulin resistance and obesity has been published. We have now extended this area of research and have determined the effects of LPS on cell viability, insulin secretion, insulin signalling and metabolism in a clonal beta-cell line. BRIN-BD11 beta-cells were treated for 24 h with increasing concentrations of LPS. Chronic (24 h) and acute (20 min) insulin secretion, insulin content and parameters of cell metabolism and insulin signalling were determined. Incubation of BRIN-BD11 cells for 24 h in the presence of increasing concentrations of the TLR4 ligand LPS significantly decreased chronic (24 h) insulin secretion from 1.09+/-0.19 to 0.76+/-0.18 microg insulin/mg protein in the presence of 100 ng/ml LPS (P

AB - Evidence for involvement of toll-like receptors (TLRs) (e.g. TLR4 and TLR2, whose agonists include lipopolysaccharides (LPS) and saturated fatty acids) in altered patterns of signalling in adipose, liver and muscle from animal models of insulin resistance and obesity has been published. We have now extended this area of research and have determined the effects of LPS on cell viability, insulin secretion, insulin signalling and metabolism in a clonal beta-cell line. BRIN-BD11 beta-cells were treated for 24 h with increasing concentrations of LPS. Chronic (24 h) and acute (20 min) insulin secretion, insulin content and parameters of cell metabolism and insulin signalling were determined. Incubation of BRIN-BD11 cells for 24 h in the presence of increasing concentrations of the TLR4 ligand LPS significantly decreased chronic (24 h) insulin secretion from 1.09+/-0.19 to 0.76+/-0.18 microg insulin/mg protein in the presence of 100 ng/ml LPS (P

U2 - 10.1677/JOE-09-0160

DO - 10.1677/JOE-09-0160

M3 - Article

VL - 202

SP - 365

EP - 373

JO - Journal of Endrocrinology

T2 - Journal of Endrocrinology

JF - Journal of Endrocrinology

SN - 0022-0795

IS - 3

ER -