Time-correlation between membrane depolarization and intracellular calcium in insulin secreting BRIN-BD11 cells: studies using FLIPR

JC Miguel, Steven Patterson, Yasser Abdel-Wahab, PCF Mathias, Peter Flatt

Research output: Contribution to journalArticle

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Abstract

Cytoplasmic Ca2+ ([Ca2+](i)) and membrane potential changes were measured in clonal pancreatic beta cells using a fluorimetric imaging plate reader (FLIPR). KCl (30 mM) produced a fast membrane depolarization immediately followed by increase of [Ca2+](i) in BRIN-BD11 cells. L-Alanine (10 mM) but not L-arginine (10 mM) mimicked the KCl profile and also produced a fast membrane depolarization and elevation of [Ca2+];. Conversely, a rise in glucose from 5.6 mM to 11.1 or 16.7 mM induced rapid membrane depolarization, followed by a slower and delayed increase of [Ca2+](i). GLP-1 (20 nM) did not affect membrane potential or [Ca2+](i). In contrast, acetylcholine (ACh, 100 muM) induced fast membrane depolarization immediately followed by a modest [Ca2+]; increase. When extracellular Ca2+ was buffered with EGTA, ACh mobilized intracellular calcium stores and the [Ca2+]; increase was reduced by 2-aminoethoxydiphenyl borate but not by dantrolene, indicating the involvement of inositol triphosphate receptors (InsP(3)R). It is concluded that membrane depolarization of beta cells by glucose stimulation is not immediately followed by elevation of [Ca2+]i and other metabolic events are involved in glucose induced stimulus-secretion coupling. It is also suggested that ACh mobilizes intracellular Ca2+ through store operated InsP(3)R. (C) 2003 Elsevier Ltd. All rights reserved.
LanguageEnglish
Pages43-50
JournalCell Calcium
Volume36
Issue number1
DOIs
Publication statusPublished - Jul 2004

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Intracellular Membranes
Insulin
Calcium
Membranes
Glucose
Membrane Potentials
Dantrolene
Inositol 1,4,5-Trisphosphate Receptors
Glucagon-Like Peptide 1
Egtazic Acid
Insulin-Secreting Cells
Alanine
Acetylcholine
Arginine

Cite this

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title = "Time-correlation between membrane depolarization and intracellular calcium in insulin secreting BRIN-BD11 cells: studies using FLIPR",
abstract = "Cytoplasmic Ca2+ ([Ca2+](i)) and membrane potential changes were measured in clonal pancreatic beta cells using a fluorimetric imaging plate reader (FLIPR). KCl (30 mM) produced a fast membrane depolarization immediately followed by increase of [Ca2+](i) in BRIN-BD11 cells. L-Alanine (10 mM) but not L-arginine (10 mM) mimicked the KCl profile and also produced a fast membrane depolarization and elevation of [Ca2+];. Conversely, a rise in glucose from 5.6 mM to 11.1 or 16.7 mM induced rapid membrane depolarization, followed by a slower and delayed increase of [Ca2+](i). GLP-1 (20 nM) did not affect membrane potential or [Ca2+](i). In contrast, acetylcholine (ACh, 100 muM) induced fast membrane depolarization immediately followed by a modest [Ca2+]; increase. When extracellular Ca2+ was buffered with EGTA, ACh mobilized intracellular calcium stores and the [Ca2+]; increase was reduced by 2-aminoethoxydiphenyl borate but not by dantrolene, indicating the involvement of inositol triphosphate receptors (InsP(3)R). It is concluded that membrane depolarization of beta cells by glucose stimulation is not immediately followed by elevation of [Ca2+]i and other metabolic events are involved in glucose induced stimulus-secretion coupling. It is also suggested that ACh mobilizes intracellular Ca2+ through store operated InsP(3)R. (C) 2003 Elsevier Ltd. All rights reserved.",
author = "JC Miguel and Steven Patterson and Yasser Abdel-Wahab and PCF Mathias and Peter Flatt",
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Time-correlation between membrane depolarization and intracellular calcium in insulin secreting BRIN-BD11 cells: studies using FLIPR. / Miguel, JC; Patterson, Steven; Abdel-Wahab, Yasser; Mathias, PCF; Flatt, Peter.

In: Cell Calcium, Vol. 36, No. 1, 07.2004, p. 43-50.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Time-correlation between membrane depolarization and intracellular calcium in insulin secreting BRIN-BD11 cells: studies using FLIPR

AU - Miguel, JC

AU - Patterson, Steven

AU - Abdel-Wahab, Yasser

AU - Mathias, PCF

AU - Flatt, Peter

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AB - Cytoplasmic Ca2+ ([Ca2+](i)) and membrane potential changes were measured in clonal pancreatic beta cells using a fluorimetric imaging plate reader (FLIPR). KCl (30 mM) produced a fast membrane depolarization immediately followed by increase of [Ca2+](i) in BRIN-BD11 cells. L-Alanine (10 mM) but not L-arginine (10 mM) mimicked the KCl profile and also produced a fast membrane depolarization and elevation of [Ca2+];. Conversely, a rise in glucose from 5.6 mM to 11.1 or 16.7 mM induced rapid membrane depolarization, followed by a slower and delayed increase of [Ca2+](i). GLP-1 (20 nM) did not affect membrane potential or [Ca2+](i). In contrast, acetylcholine (ACh, 100 muM) induced fast membrane depolarization immediately followed by a modest [Ca2+]; increase. When extracellular Ca2+ was buffered with EGTA, ACh mobilized intracellular calcium stores and the [Ca2+]; increase was reduced by 2-aminoethoxydiphenyl borate but not by dantrolene, indicating the involvement of inositol triphosphate receptors (InsP(3)R). It is concluded that membrane depolarization of beta cells by glucose stimulation is not immediately followed by elevation of [Ca2+]i and other metabolic events are involved in glucose induced stimulus-secretion coupling. It is also suggested that ACh mobilizes intracellular Ca2+ through store operated InsP(3)R. (C) 2003 Elsevier Ltd. All rights reserved.

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