Through the eye of an electrospray needle: mass spectrometric identification of themajor peptides and proteins in the milk of the one-humped camel (Camelus dromedarius)

A Alhaider, AG Abdelgader, AA Turjoman, K Newell, SW Hunsucker, B Shan, B Ma, DS Gibson, MW Duncan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The milk of the one-humped camel (Camelus dromedarius) reportedly offers medicinal benefits, perhaps because of its unique bioactive components. Milk proteins were determined by (1) two-dimensional gel electrophoresis and peptide mass mapping and (2) liquid chromatography-tandem mass spectrometry (LC-MS/MS) following one-dimensional polyacrylamide gel electrophoresis. Over 200 proteins were identified: some known camel proteins including heavy-chain immunoglobulins and others exhibiting regions of exact homology with proteins from other species. Indigenous peptides were also identified following isolation and concentration by two strategies: (1) gel-eluted liquid fraction entrapment electrophoresis and (2) small-scale electrophoretic separation. Extracts were analyzed by LC-MS/MS and peptides identified by matching strategies, by de novo sequencing and by applying a sequence tag tool requiring similarity to the proposed sequence, but not an exact match. A plethora of protein cleavage products including some novel peptides were characterized. These studies demonstrate that camel milk is a rich source of peptides, some of which may serve as nutraceuticals.
Original languageEnglish
Pages (from-to)779-794
JournalJournal of Mass Spectrometry
Volume48
Issue number7
DOIs
Publication statusPublished - Jul 2013

Keywords

  • Camel milk
  • de novo peptide sequencing
  • mass spectrometry
  • proteomics

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