Thionin attached to a gold electrode modified with self-assembly of Mo6S9−XIXnanowires for amplified electrochemical detection of natural DNA

H Lin, H Cheng, L Liu, Z Zhu, Y Shao, P Papakonstantinou, D Mihailovic, M Li

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We demonstrate a novel electrochemical sensor for highly sensitive detection of natural double-stranded deoxyribonucleic acid (dsDNA) based on thionin (Th) attached to Mo6S9−XIX nanowires (MoSI NWs) self-assembled on a gold electrode. The sensing detection is based on a decrease of the voltammetric response of the immobilized Th due to the binding of Th with dsDNA through intercalation. MoSI NWs act as molecular connectors to provide an amplification and conductive sensing platform for the electrochem-ical detection of dsDNA, because many sulfur atoms at the ends and sides of MoSI NWs permit covalent bonds to be formed with gold as well as MoSI NWs with negative charges allow electrostatic binding with Th. A determination limit of 0.62 ng/mL for dsDNAwith this novel sensor is achieved,which is three orders ofmagnitude lower than that in the absence ofMoSI NWs. The operation is simple and label-free.
LanguageEnglish
Pages1866-1870
JournalBiosensors and Bioelectronics
Volume26
DOIs
Publication statusPublished - Jan 2011

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Thionins
Nanowires
Gold
Self assembly
Electrodes
DNA
Electrochemical sensors
Covalent bonds
Intercalation
Static Electricity
Sulfur
Amplification
Labels
Electrostatics
Atoms
Sensors

Cite this

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title = "Thionin attached to a gold electrode modified with self-assembly of Mo6S9−XIXnanowires for amplified electrochemical detection of natural DNA",
abstract = "We demonstrate a novel electrochemical sensor for highly sensitive detection of natural double-stranded deoxyribonucleic acid (dsDNA) based on thionin (Th) attached to Mo6S9−XIX nanowires (MoSI NWs) self-assembled on a gold electrode. The sensing detection is based on a decrease of the voltammetric response of the immobilized Th due to the binding of Th with dsDNA through intercalation. MoSI NWs act as molecular connectors to provide an amplification and conductive sensing platform for the electrochem-ical detection of dsDNA, because many sulfur atoms at the ends and sides of MoSI NWs permit covalent bonds to be formed with gold as well as MoSI NWs with negative charges allow electrostatic binding with Th. A determination limit of 0.62 ng/mL for dsDNAwith this novel sensor is achieved,which is three orders ofmagnitude lower than that in the absence ofMoSI NWs. The operation is simple and label-free.",
author = "H Lin and H Cheng and L Liu and Z Zhu and Y Shao and P Papakonstantinou and D Mihailovic and M Li",
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Thionin attached to a gold electrode modified with self-assembly of Mo6S9−XIXnanowires for amplified electrochemical detection of natural DNA. / Lin, H; Cheng, H; Liu, L; Zhu, Z; Shao, Y; Papakonstantinou, P; Mihailovic, D; Li, M.

In: Biosensors and Bioelectronics, Vol. 26, 01.2011, p. 1866-1870.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Thionin attached to a gold electrode modified with self-assembly of Mo6S9−XIXnanowires for amplified electrochemical detection of natural DNA

AU - Lin, H

AU - Cheng, H

AU - Liu, L

AU - Zhu, Z

AU - Shao, Y

AU - Papakonstantinou, P

AU - Mihailovic, D

AU - Li, M

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AB - We demonstrate a novel electrochemical sensor for highly sensitive detection of natural double-stranded deoxyribonucleic acid (dsDNA) based on thionin (Th) attached to Mo6S9−XIX nanowires (MoSI NWs) self-assembled on a gold electrode. The sensing detection is based on a decrease of the voltammetric response of the immobilized Th due to the binding of Th with dsDNA through intercalation. MoSI NWs act as molecular connectors to provide an amplification and conductive sensing platform for the electrochem-ical detection of dsDNA, because many sulfur atoms at the ends and sides of MoSI NWs permit covalent bonds to be formed with gold as well as MoSI NWs with negative charges allow electrostatic binding with Th. A determination limit of 0.62 ng/mL for dsDNAwith this novel sensor is achieved,which is three orders ofmagnitude lower than that in the absence ofMoSI NWs. The operation is simple and label-free.

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DO - 10.1016/j.bios.2010.01.035

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SN - 0956-5663

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