Thermostable, alkalophilic and cellulase free xylanase production by Thermoactinomyces thalophilus subgroup C

U Kohli, Poonam Singh - Nee Nigam, D Singh, K Chaudhary

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Thermoactinomyces thalophilus produced cellulase free extracellular endo-1,4-beta -xylanase (EC 3.2.1.8) at 50 degreesC and pH 8.5. Maximum xylanase production was thieved in fermentation medium using birchwood xylan as substrate after 96 h of growth at 50 degreesC. Other agricultural substrates such as wheat bran, wheat straw, sugarcane bagnsse and cornstover produced less xylanase, The crude enzyme preparation from mutant T. thalophilus P2 grown under optimised fermentation conditions showed no cellulase contamination and maximum xylanase activity of 42 U/ml at 65 degreesC and pH 8.5-9.0. This enzyme with initial sylanase activity of 42 U/ml was found thermostable up to 65 degreesC and retaining 50% of its activity after its incubation for 125 min at 65 degreesC. (C) 2001 Elsevier Science Inc. All rights reserved.
LanguageEnglish
Pages606-610
JournalEnzyme and Microbial Technology
Volume28
Issue number7-8
Publication statusPublished - May 2001

Fingerprint

Thermoactinomyces
xylanases
endo-1,4-beta-glucanase
endo-1,4-beta-xylanase
fermentation
xylan
wheat bran
wheat straw
enzymes
sugarcane
mutants

Cite this

@article{2c56885054474019b17e5338a701d9b0,
title = "Thermostable, alkalophilic and cellulase free xylanase production by Thermoactinomyces thalophilus subgroup C",
abstract = "Thermoactinomyces thalophilus produced cellulase free extracellular endo-1,4-beta -xylanase (EC 3.2.1.8) at 50 degreesC and pH 8.5. Maximum xylanase production was thieved in fermentation medium using birchwood xylan as substrate after 96 h of growth at 50 degreesC. Other agricultural substrates such as wheat bran, wheat straw, sugarcane bagnsse and cornstover produced less xylanase, The crude enzyme preparation from mutant T. thalophilus P2 grown under optimised fermentation conditions showed no cellulase contamination and maximum xylanase activity of 42 U/ml at 65 degreesC and pH 8.5-9.0. This enzyme with initial sylanase activity of 42 U/ml was found thermostable up to 65 degreesC and retaining 50{\%} of its activity after its incubation for 125 min at 65 degreesC. (C) 2001 Elsevier Science Inc. All rights reserved.",
author = "U Kohli and {Singh - Nee Nigam}, Poonam and D Singh and K Chaudhary",
year = "2001",
month = "5",
language = "English",
volume = "28",
pages = "606--610",
journal = "Enzyme and Microbial Technology",
issn = "0141-0229",
publisher = "Elsevier",
number = "7-8",

}

Thermostable, alkalophilic and cellulase free xylanase production by Thermoactinomyces thalophilus subgroup C. / Kohli, U; Singh - Nee Nigam, Poonam; Singh, D; Chaudhary, K.

In: Enzyme and Microbial Technology, Vol. 28, No. 7-8, 05.2001, p. 606-610.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Thermostable, alkalophilic and cellulase free xylanase production by Thermoactinomyces thalophilus subgroup C

AU - Kohli, U

AU - Singh - Nee Nigam, Poonam

AU - Singh, D

AU - Chaudhary, K

PY - 2001/5

Y1 - 2001/5

N2 - Thermoactinomyces thalophilus produced cellulase free extracellular endo-1,4-beta -xylanase (EC 3.2.1.8) at 50 degreesC and pH 8.5. Maximum xylanase production was thieved in fermentation medium using birchwood xylan as substrate after 96 h of growth at 50 degreesC. Other agricultural substrates such as wheat bran, wheat straw, sugarcane bagnsse and cornstover produced less xylanase, The crude enzyme preparation from mutant T. thalophilus P2 grown under optimised fermentation conditions showed no cellulase contamination and maximum xylanase activity of 42 U/ml at 65 degreesC and pH 8.5-9.0. This enzyme with initial sylanase activity of 42 U/ml was found thermostable up to 65 degreesC and retaining 50% of its activity after its incubation for 125 min at 65 degreesC. (C) 2001 Elsevier Science Inc. All rights reserved.

AB - Thermoactinomyces thalophilus produced cellulase free extracellular endo-1,4-beta -xylanase (EC 3.2.1.8) at 50 degreesC and pH 8.5. Maximum xylanase production was thieved in fermentation medium using birchwood xylan as substrate after 96 h of growth at 50 degreesC. Other agricultural substrates such as wheat bran, wheat straw, sugarcane bagnsse and cornstover produced less xylanase, The crude enzyme preparation from mutant T. thalophilus P2 grown under optimised fermentation conditions showed no cellulase contamination and maximum xylanase activity of 42 U/ml at 65 degreesC and pH 8.5-9.0. This enzyme with initial sylanase activity of 42 U/ml was found thermostable up to 65 degreesC and retaining 50% of its activity after its incubation for 125 min at 65 degreesC. (C) 2001 Elsevier Science Inc. All rights reserved.

M3 - Article

VL - 28

SP - 606

EP - 610

JO - Enzyme and Microbial Technology

T2 - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 7-8

ER -