The use of oxygen uptake rate measurements to control the supply of toxic substrate: toluene hydroxylation by Pseudomonas putida UV4.

Catherine Hack

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above ( approximately 2.4 mmol), is fed to the reactor. A feed-back control system based on oxygen uptake rate measurements was used to control the feed rate, and thus maintain the aqueous phase toluene concentration in the desired range for zero order kinetics.
    LanguageEnglish
    Pages183-188
    JournalEnzyme and Microbial Technology
    Volume28
    Issue number2-3
    Publication statusPublished - 2001

    Fingerprint

    Hydroxylation
    Pseudomonas putida
    Poisons
    Toluene
    Oxygen
    Substrates
    Glycols
    Feedback control
    Control systems
    Molecules
    Kinetics

    Cite this

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    title = "The use of oxygen uptake rate measurements to control the supply of toxic substrate: toluene hydroxylation by Pseudomonas putida UV4.",
    abstract = "During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above ( approximately 2.4 mmol), is fed to the reactor. A feed-back control system based on oxygen uptake rate measurements was used to control the feed rate, and thus maintain the aqueous phase toluene concentration in the desired range for zero order kinetics.",
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    year = "2001",
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    pages = "183--188",
    journal = "Enzyme and Microbial Technology",
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    The use of oxygen uptake rate measurements to control the supply of toxic substrate: toluene hydroxylation by Pseudomonas putida UV4. / Hack, Catherine.

    In: Enzyme and Microbial Technology, Vol. 28, No. 2-3, 2001, p. 183-188.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - The use of oxygen uptake rate measurements to control the supply of toxic substrate: toluene hydroxylation by Pseudomonas putida UV4.

    AU - Hack, Catherine

    PY - 2001

    Y1 - 2001

    N2 - During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above ( approximately 2.4 mmol), is fed to the reactor. A feed-back control system based on oxygen uptake rate measurements was used to control the feed rate, and thus maintain the aqueous phase toluene concentration in the desired range for zero order kinetics.

    AB - During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above ( approximately 2.4 mmol), is fed to the reactor. A feed-back control system based on oxygen uptake rate measurements was used to control the feed rate, and thus maintain the aqueous phase toluene concentration in the desired range for zero order kinetics.

    M3 - Article

    VL - 28

    SP - 183

    EP - 188

    JO - Enzyme and Microbial Technology

    T2 - Enzyme and Microbial Technology

    JF - Enzyme and Microbial Technology

    SN - 0141-0229

    IS - 2-3

    ER -