The use of oxygen uptake rate measurements to control the supply of toxic substrate: toluene hydroxylation by Pseudomonas putida UV4.

Catherine Hack

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above ( approximately 2.4 mmol), is fed to the reactor. A feed-back control system based on oxygen uptake rate measurements was used to control the feed rate, and thus maintain the aqueous phase toluene concentration in the desired range for zero order kinetics.
    Original languageEnglish
    Pages (from-to)183-188
    JournalEnzyme and Microbial Technology
    Volume28
    Issue number2-3
    Publication statusPublished - 2001

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