The use of low-cost brewery waste product for the production of surfactin as a natural microbial biocide

Talita Correa  Nazareth; Canrado Planas Zanutto; Lakshmi Tripathi; Abdulaziz Juma; Danielle Maass; Antonio Augusto De Douza; Selene Maria de Arruda Guelli De Souza; Ibrahim Banat

doi:10.1016/j.btre.2020.e00537

The use of low-cost brewery waste product for the production of surfactin as a natural microbial biocide

Talita Correa Nazareth, Canrado Planas Zanutto, Lakshmi Tripathi, Abdulaziz Juma, Danielle Maass, Antonio Augusto De Douza, Selene Maria de Arruda Guelli De Souza, Ibrahim Banat

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Abstract

Surfactin has potential as next generation antibiofilm agent to combat antimicrobial resistance against emerging pathogens. However, the widespread industrial applications of surfactin is hampered by its high production cost. In this work, surfactin was produced from Bacillus subtilis using a low-cost brewery waste as a carbon source. The strain produced 210.11 mg L ⁻¹ after 28 h. The antimicrobial activity was observed against all tested strains, achieving complete inhibition for Pseudomonas aeruginosa, at 500 μg mL ⁻¹. A growth log reduction of 3.91 was achieved for P. aeruginosa while, Staphylococcus aureus and Staphylococcus epidermidis showed between 1 and 2 log reductions. In the anti-biofilm assays against P. aeruginosa, the co-incubation, anti-adhesive and disruption showed inhibition, where the greatest inhibition was observed in the co-incubation assay (79.80%). This study provides evidence that surfactin produced from a low-cost substrate can be a promising biocide due to its antimicrobial and anti-biofilm abilities against pathogens.

Original language	English
Article number	e00537
Number of pages	10
Journal	Biotechnology Reports
Volume	28
Early online date	16 Oct 2020
DOIs	https://doi.org/10.1016/j.btre.2020.e00537
Publication status	Published (in print/issue) - 31 Dec 2020

Bibliographical note

Funding Information:
The authors gratefully acknowledge the financial support of Amazonas Research Foundation ( FAPEAM ), Microbiology Laboratory of Ulster University and Laboratory of Mass Transfer (LABMASSA) of Federal University of Santa Catarina. Danielle Maass is thankful to São Paulo Research Foundation ( FAPESP ) for the research grant 2019/07659-4 .

Publisher Copyright:
© 2020 The Authors

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

Adhesion
Biofilm
Biosurfactants
Brewery waste
Surfactin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.btre.2020.e00537

Nazareth et al 2020 Biotechnology ReportsFinal published version, 2.23 MBLicence: CC BY-NC-ND

Cite this

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abstract = "Surfactin has potential as next generation antibiofilm agent to combat antimicrobial resistance against emerging pathogens. However, the widespread industrial applications of surfactin is hampered by its high production cost. In this work, surfactin was produced from Bacillus subtilis using a low-cost brewery waste as a carbon source. The strain produced 210.11 mg L −1 after 28 h. The antimicrobial activity was observed against all tested strains, achieving complete inhibition for Pseudomonas aeruginosa, at 500 μg mL −1. A growth log reduction of 3.91 was achieved for P. aeruginosa while, Staphylococcus aureus and Staphylococcus epidermidis showed between 1 and 2 log reductions. In the anti-biofilm assays against P. aeruginosa, the co-incubation, anti-adhesive and disruption showed inhibition, where the greatest inhibition was observed in the co-incubation assay (79.80%). This study provides evidence that surfactin produced from a low-cost substrate can be a promising biocide due to its antimicrobial and anti-biofilm abilities against pathogens. ",

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AU - Maass, Danielle

AU - De Douza, Antonio Augusto

AU - De Souza, Selene Maria de Arruda Guelli

AU - Banat, Ibrahim

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N2 - Surfactin has potential as next generation antibiofilm agent to combat antimicrobial resistance against emerging pathogens. However, the widespread industrial applications of surfactin is hampered by its high production cost. In this work, surfactin was produced from Bacillus subtilis using a low-cost brewery waste as a carbon source. The strain produced 210.11 mg L −1 after 28 h. The antimicrobial activity was observed against all tested strains, achieving complete inhibition for Pseudomonas aeruginosa, at 500 μg mL −1. A growth log reduction of 3.91 was achieved for P. aeruginosa while, Staphylococcus aureus and Staphylococcus epidermidis showed between 1 and 2 log reductions. In the anti-biofilm assays against P. aeruginosa, the co-incubation, anti-adhesive and disruption showed inhibition, where the greatest inhibition was observed in the co-incubation assay (79.80%). This study provides evidence that surfactin produced from a low-cost substrate can be a promising biocide due to its antimicrobial and anti-biofilm abilities against pathogens.

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The use of low-cost brewery waste product for the production of surfactin as a natural microbial biocide

Abstract

Bibliographical note

Keywords

UN SDGs

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