TY - JOUR
T1 - The thymidylate synthase tandem repeat polymorphism is not associated with homocysteine concentrations in healthy young subjects
AU - Brown, Karen S.
AU - Kluijtmans, Leo A.J.
AU - Young, Ian S.
AU - McNulty, Helene
AU - Mitchell, Laura E.
AU - Yarnell, John W.G.
AU - Woodside, Jayne V.
AU - Boreham, Colin A.
AU - McMaster, Dorothy
AU - Murray, Liam
AU - Strain, J. J.
AU - Whitehead, Alexander S.
PY - 2004/1
Y1 - 2004/1
N2 - Thymidylate synthase (TYMS) and 5,10-methylenetetrahydrofolate reductase (MTHFR) may compete for their common cofactor 5,10-methylenetetrahyhdrofolate (5,10-meTHF). Limiting 5,10-meTHF results in elevated homocysteine, especially in individuals homozygous for the T allele of the MTHFR C677T polymorphism. The TYMS gene has a tandem repeat polymorphism (two repeats or three repeats, designated 2R or 3R, respectively), which may also affect homocysteine concentrations. The 3R allele is subject to increased translational efficiency in vitro and the 3R3R genotype is associated with both decreased serum folate and elevated plasma homocysteine (tHcy) in a population of Singapore Chinese. We assessed the relationship between TYMS genotype and key biochemical and genetic variables in a random sample of 392 healthy young Northwestern European men and women. The tHcy concentrations for 3R3R homozygotes (median 8.5 μmol/l) did not differ significantly from those for 2R2R homozygotes (median 8.7 μmol/l) or 2R3R heterozygotes (median 9.3μmol/l) in the population as a whole (P=0.43), or in subsets of subjects with low serum folate (P=0.60) or the MTHFR 677TT genotype (P=0.90). Furthermore, there was no trend toward elevated tHcy in 3R3R homozygotes. Similarly, the TYMS tandem repeat polymorphism was not associated with serum folate concentrations. Our findings indicate that the TYMS 3R3R genotype is not a determinant of homocysteine in this sample of healthy young Caucasian adults from Northern Ireland.
AB - Thymidylate synthase (TYMS) and 5,10-methylenetetrahydrofolate reductase (MTHFR) may compete for their common cofactor 5,10-methylenetetrahyhdrofolate (5,10-meTHF). Limiting 5,10-meTHF results in elevated homocysteine, especially in individuals homozygous for the T allele of the MTHFR C677T polymorphism. The TYMS gene has a tandem repeat polymorphism (two repeats or three repeats, designated 2R or 3R, respectively), which may also affect homocysteine concentrations. The 3R allele is subject to increased translational efficiency in vitro and the 3R3R genotype is associated with both decreased serum folate and elevated plasma homocysteine (tHcy) in a population of Singapore Chinese. We assessed the relationship between TYMS genotype and key biochemical and genetic variables in a random sample of 392 healthy young Northwestern European men and women. The tHcy concentrations for 3R3R homozygotes (median 8.5 μmol/l) did not differ significantly from those for 2R2R homozygotes (median 8.7 μmol/l) or 2R3R heterozygotes (median 9.3μmol/l) in the population as a whole (P=0.43), or in subsets of subjects with low serum folate (P=0.60) or the MTHFR 677TT genotype (P=0.90). Furthermore, there was no trend toward elevated tHcy in 3R3R homozygotes. Similarly, the TYMS tandem repeat polymorphism was not associated with serum folate concentrations. Our findings indicate that the TYMS 3R3R genotype is not a determinant of homocysteine in this sample of healthy young Caucasian adults from Northern Ireland.
UR - http://www.scopus.com/inward/record.url?scp=10744222721&partnerID=8YFLogxK
U2 - 10.1007/s00439-003-1039-9
DO - 10.1007/s00439-003-1039-9
M3 - Article
C2 - 14586640
AN - SCOPUS:10744222721
SN - 0340-6717
VL - 114
SP - 182
EP - 185
JO - Human Genetics
JF - Human Genetics
IS - 2
ER -