The Potential Role of Lipopeptide Biosurfactant Generated by Acinetobacter junii B6 on Leishmania Tropica: The Synergy of Lipopeptide Biosurfactant and Glucantime

Fatemeh Sharifi, Iraj Sharifi, Mandana Ohadi, Neda Mohamadi, Ehsan Salarkia, Ibrahim M Banat, Gholamreza Dehghannoudeh

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Abstract

An in vitro investigation was carried out to assess how the lipopeptide biosurfactant (LPB) produced by Acinetobacter junii B6 affects Leishmania tropica infection and the associated cytokine gene expression in macrophages infected with L. tropica. Glucantime® (meglumine antimoniate, MA) and LPB were investigated for their leishmanicidal effect, alone and in combination (LPB + MA), using a colorimetric test and a macrophage model. Immunomodulatory impact was also evaluated through analysis of Th1 and Th2 cytokine gene expression in infected macrophages after treatment with MA and LPB, individually and in combination. The MA/LPB combination showed higher inhibitory impacts on L. tropica amastigotes and promastigotes than each alone. Cytokine gene expression confirmed LPB’s affinity to IFN-γ, affirming the elevated IL-12p40 and IFN- γ concentrations in addition to a reduction in the secretion of IL-10 in a dose-dependent manner, particularly in combined treatment. The results indicated higher effectiveness of LPB along with MA in the reduction of the parasite growth and promoting the immune reaction level, which may be considered as a possible therapeutic strategy to treat those with anthroponotic cutaneous leishmaniasis.
Original languageEnglish
Article number57
Number of pages8
JournalInternational Journal of Peptide Research and Therapeutics
Volume29
Issue number4
Early online date19 May 2023
DOIs
Publication statusPublished online - 19 May 2023

Bibliographical note

Funding Information:
This work was supported by the Kerman University of Medical Sciences under grant number 402000027.

Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Nature B.V.

Keywords

  • Lipopeptide biosurfactant
  • Acinetobacter junii B6
  • Leishmania tropica
  • Glucantime

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