The petroleum-degrading bacteria Alcaligenes aquatilis strain YGD 2906 as a potential source of lipopeptide biosurfactant

Drifa Yalaoui-Guellal, Samira Fella-Temzi, Salima Djafri-Dib, Sunil Kumar Sahu, Victor U. Irorere, Ibrahim M. Banat, Khodir Madani

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Soummam river sediments were used to isolate a biosurfactant-producing and petroleum-degrading bacterium. The strain was identified as Alcaligenes aquatilis YGD 2906 using phenotypic characterization and 16S ribosomal RNA sequencing. The culture supernatant of the isolated strain showed no haemolytic activity had an oiled displacement of 23.66 ± 0.57 mm and Emulsification index (E24) of 68.5% ± 0.5%. The biosurfactant produced in the minimal medium was extracted by acid precipitation and quantified gravimetrically, with a yield of 4.2 ± 0.01 g/L. The crude Biosurfactant was determinate by TLC and MALDI-TOF-MS as a lipopeptide with protein and lipid content of 8.49 ± 0.19% and 52.66 ± 1.16% respectively. This lipopeptide structure was confirmed by HPLC-MS/MS. This technique gave two main peak ranges which are typical of surfactins, iturins and fengycin. Tandem mass spectrometry was further used to elucidate the structure of the lipopeptide produced by the strain. The non-haemolytic nature of the lipopeptide produced by this strain offers opportunities for biomedical applications. Further work is needed to optimize production and analyze potential biomedical uses of this lipopeptide including antimicrobial properties.

Original languageEnglish
Article number119112
Pages (from-to)1-7
Number of pages7
Early online date8 Sept 2020
Publication statusPublished (in print/issue) - 1 Feb 2021

Bibliographical note

Funding Information:
This study was supported by a grant from the Ministry of High Education and Scientific Research of Algeria and University of Ulster (Northern Ireland, UK). All authors thank everyone who contributed in the successful completion of to the realization of this project. We would like to thank Penedo P.M. of laboratory Stab VIDA (Portugal) for helping in the molecular identification of the studied species.

Publisher Copyright:
© 2020 Elsevier Ltd


  • Alcaligenes aquatilis strain YGD 2906
  • Biosurfactant production
  • MALDI-TOF/MS analysis
  • Petroleum biodegradation


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