The pattern of pleiomorphism in stressed Salmonella Virchow populations is nutrient and growth phase dependent

M. A. S. McMahon, D. A. McDowell, I. S. Blair

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Aims: To describe the interactions of imposed osmotic and nutritional stress on the morphology of stationary and exponential phase S. Virchow cells. Methods and Results: This study examined the morphology and viability of osmotically stressed exponential and stationary phase cultures of Salmonella Virchow under nutritionally deficient and competent conditions. In addition to normal morphology, salt-stressed cultures exhibited filamentous and spherical morphotypes, which were capable of reversion to normal morphology on stress removal. Proportions of atypical morphotypes were influenced by the phase of growth when the stress was applied. Salt-stressed exponential phase populations contained 54% filamentous, 30% spherical forms, salt-stressed stationary phase populations contained 16% filamentous, 79% spherical forms. Proportions of morphotypes were also influenced by the nutrient status of the medium, but not by metabolic by-products. Conclusion: Development of a range of morphotypes in response to stress (osmotic/nutritional), may offer population level advantages, increasing the survival potential of the population. Significance and Impact of the Study: The application of sublethal concentrations of salt may stimulate S. Virchow morphotype diversity, improving survival and rates of poststress recovery.
    LanguageEnglish
    Pages276-281
    JournalLetters in Applied Microbiology
    Volume45
    Issue number3
    DOIs
    Publication statusPublished - Sep 2007

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    Salmonella
    Salts
    Food
    Growth
    Population
    Osmoregulation
    Osmotic Pressure
    Population Dynamics
    S Phase
    Survival Rate

    Keywords

    • filamentous cells
    • morphotypes
    • nutrients
    • osmotic stress
    • Salmonella Virchow
    • spherical cells

    Cite this

    McMahon, M. A. S. ; McDowell, D. A. ; Blair, I. S. / The pattern of pleiomorphism in stressed Salmonella Virchow populations is nutrient and growth phase dependent. In: Letters in Applied Microbiology. 2007 ; Vol. 45, No. 3. pp. 276-281.
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    abstract = "Aims: To describe the interactions of imposed osmotic and nutritional stress on the morphology of stationary and exponential phase S. Virchow cells. Methods and Results: This study examined the morphology and viability of osmotically stressed exponential and stationary phase cultures of Salmonella Virchow under nutritionally deficient and competent conditions. In addition to normal morphology, salt-stressed cultures exhibited filamentous and spherical morphotypes, which were capable of reversion to normal morphology on stress removal. Proportions of atypical morphotypes were influenced by the phase of growth when the stress was applied. Salt-stressed exponential phase populations contained 54{\%} filamentous, 30{\%} spherical forms, salt-stressed stationary phase populations contained 16{\%} filamentous, 79{\%} spherical forms. Proportions of morphotypes were also influenced by the nutrient status of the medium, but not by metabolic by-products. Conclusion: Development of a range of morphotypes in response to stress (osmotic/nutritional), may offer population level advantages, increasing the survival potential of the population. Significance and Impact of the Study: The application of sublethal concentrations of salt may stimulate S. Virchow morphotype diversity, improving survival and rates of poststress recovery.",
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    The pattern of pleiomorphism in stressed Salmonella Virchow populations is nutrient and growth phase dependent. / McMahon, M. A. S.; McDowell, D. A.; Blair, I. S.

    In: Letters in Applied Microbiology, Vol. 45, No. 3, 09.2007, p. 276-281.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - McMahon, M. A. S.

    AU - McDowell, D. A.

    AU - Blair, I. S.

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    N2 - Aims: To describe the interactions of imposed osmotic and nutritional stress on the morphology of stationary and exponential phase S. Virchow cells. Methods and Results: This study examined the morphology and viability of osmotically stressed exponential and stationary phase cultures of Salmonella Virchow under nutritionally deficient and competent conditions. In addition to normal morphology, salt-stressed cultures exhibited filamentous and spherical morphotypes, which were capable of reversion to normal morphology on stress removal. Proportions of atypical morphotypes were influenced by the phase of growth when the stress was applied. Salt-stressed exponential phase populations contained 54% filamentous, 30% spherical forms, salt-stressed stationary phase populations contained 16% filamentous, 79% spherical forms. Proportions of morphotypes were also influenced by the nutrient status of the medium, but not by metabolic by-products. Conclusion: Development of a range of morphotypes in response to stress (osmotic/nutritional), may offer population level advantages, increasing the survival potential of the population. Significance and Impact of the Study: The application of sublethal concentrations of salt may stimulate S. Virchow morphotype diversity, improving survival and rates of poststress recovery.

    AB - Aims: To describe the interactions of imposed osmotic and nutritional stress on the morphology of stationary and exponential phase S. Virchow cells. Methods and Results: This study examined the morphology and viability of osmotically stressed exponential and stationary phase cultures of Salmonella Virchow under nutritionally deficient and competent conditions. In addition to normal morphology, salt-stressed cultures exhibited filamentous and spherical morphotypes, which were capable of reversion to normal morphology on stress removal. Proportions of atypical morphotypes were influenced by the phase of growth when the stress was applied. Salt-stressed exponential phase populations contained 54% filamentous, 30% spherical forms, salt-stressed stationary phase populations contained 16% filamentous, 79% spherical forms. Proportions of morphotypes were also influenced by the nutrient status of the medium, but not by metabolic by-products. Conclusion: Development of a range of morphotypes in response to stress (osmotic/nutritional), may offer population level advantages, increasing the survival potential of the population. Significance and Impact of the Study: The application of sublethal concentrations of salt may stimulate S. Virchow morphotype diversity, improving survival and rates of poststress recovery.

    KW - filamentous cells

    KW - morphotypes

    KW - nutrients

    KW - osmotic stress

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    KW - spherical cells

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