Hematopoietic stem cells derived from fetal liver have promising therapeutic potential for allotransplantation but require a specific protocol to minimize the damage produced by cryopreservation procedures. In this study, a fundamental approach was applied for designing a cell preservation protocol. To this end, the biophysical characteristics that describe the osmotic reaction of CD34+CD38- human fetal liver stem cell candidates were studied using fluorescent microscopy. The osmotically inactive volume of the stem cell candidates was determined as 48% of the isotonic volume. The permeability coefficients for water and Me2SO were determined at T=+22°C: Lp=0.27±0.03μmmin-1atm -1, PMe2SO=2.09±0.85×10 -4cmmin-1, σMe2SO=0.63±0.03 and at T=+12°C: Lp=0.15±0.02μmmin-1atm -1, PMe2SO=6.44±1.42×10 -5cmmin-1, σMe2SO=0.46±0.05. The results obtained suggest that post-hypertonic and hypotonic stress are the possible reasons for damage to a CD34+CD38- cell during the cryopreservation procedure.
- Hematopoietic stem cells
- Osmotic characteristics