The osmotic characteristics of human fetal liver-derived hematopoietic stem cell candidates

A. I. Tarasov, A. Yu Petrenko, D. R.E. Jones

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Hematopoietic stem cells derived from fetal liver have promising therapeutic potential for allotransplantation but require a specific protocol to minimize the damage produced by cryopreservation procedures. In this study, a fundamental approach was applied for designing a cell preservation protocol. To this end, the biophysical characteristics that describe the osmotic reaction of CD34+CD38- human fetal liver stem cell candidates were studied using fluorescent microscopy. The osmotically inactive volume of the stem cell candidates was determined as 48% of the isotonic volume. The permeability coefficients for water and Me2SO were determined at T=+22°C: Lp=0.27±0.03μmmin-1atm -1, PMe2SO=2.09±0.85×10 -4cmmin-1, σMe2SO=0.63±0.03 and at T=+12°C: Lp=0.15±0.02μmmin-1atm -1, PMe2SO=6.44±1.42×10 -5cmmin-1, σMe2SO=0.46±0.05. The results obtained suggest that post-hypertonic and hypotonic stress are the possible reasons for damage to a CD34+CD38- cell during the cryopreservation procedure.

Original languageEnglish
Pages (from-to)333-340
Number of pages8
JournalCryobiology
Volume48
Issue number3
DOIs
Publication statusPublished - 1 Jun 2004

Keywords

  • Cryopreservation
  • Hematopoietic stem cells
  • Osmotic characteristics

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