TY - JOUR
T1 - The nature of big plasma somatostatin
T2 - Implications for the measurement of somatostatin-like immunoreactivity in human plasma
AU - Conlon, J. Michael
AU - Bridgeman, Myrtle
AU - Alberti, K. George M.M.
PY - 1982/9/15
Y1 - 1982/9/15
N2 - Big plasma somatostatin (BPS) represents an artifact of measurement. High-molecular-weight globulins (α, β, and γ) in human plasma inhibit, in a concentration-dependent manner, the binding of radiolabeled somatostatin analogs to antibody directed against somatostatin. The magnitude of inhibition varies with antibody and plasma sample and is greatest for the α-globulin fraction. The mechanism of inhibition involves binding of plasma globulins to antibody, thereby blocking tracer-binding sites, and does not involve inhibition by somatostatin bound noncovalently to plasma proteins or tracer degradation. Thus BPS arises from a property of plasma rather than of somatostatin and so it is suggested that this mechanism may account for the presence of other "big" forms of hormones in plasma.
AB - Big plasma somatostatin (BPS) represents an artifact of measurement. High-molecular-weight globulins (α, β, and γ) in human plasma inhibit, in a concentration-dependent manner, the binding of radiolabeled somatostatin analogs to antibody directed against somatostatin. The magnitude of inhibition varies with antibody and plasma sample and is greatest for the α-globulin fraction. The mechanism of inhibition involves binding of plasma globulins to antibody, thereby blocking tracer-binding sites, and does not involve inhibition by somatostatin bound noncovalently to plasma proteins or tracer degradation. Thus BPS arises from a property of plasma rather than of somatostatin and so it is suggested that this mechanism may account for the presence of other "big" forms of hormones in plasma.
UR - http://www.scopus.com/inward/record.url?scp=0020404154&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(82)90002-1
DO - 10.1016/0003-2697(82)90002-1
M3 - Article
C2 - 6129817
AN - SCOPUS:0020404154
SN - 0003-2697
VL - 125
SP - 243
EP - 252
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -