Abstract
| Language | English |
|---|---|
| Pages | 378-381 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 37 |
| Issue number | 3 |
| Publication status | Published - Jun 1992 |
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THE ISOLATION AND CHARACTERIZATION OF A SALICYLATE-HYDROXYLASE-PRODUCING STRAIN OF PSEUDOMONAS-PUTIDA. / Banat, IM; Singh - Nee Nigam, Poonam; Marchant, R.
In: Applied Microbiology and Biotechnology, Vol. 37, No. 3, 06.1992, p. 378-381.Research output: Contribution to journal › Article
TY - JOUR
T1 - THE ISOLATION AND CHARACTERIZATION OF A SALICYLATE-HYDROXYLASE-PRODUCING STRAIN OF PSEUDOMONAS-PUTIDA
AU - Banat, IM
AU - Singh - Nee Nigam, Poonam
AU - Marchant, R
PY - 1992/6
Y1 - 1992/6
N2 - A salicylate-hydroxylase-producing strain of Pseudomonas putida with an unusual capability to grow at toxic levels of salicylate up to 10 g l-1 has been isolated. It grew well under continuous culture conditions, with optimum growth at pH 6.5 and a temperature of 25-degrees-C. The use of an ammonium salt as a nitrogen source, instead of nitrate, resulted in a 30-40% increase in its biomass yield coefficient. Optimum growth under continuous culture conditions was achieved using 4 g l-1 salicylate at 25-degrees-C, pH 6.5 and 0.2 h-1 dilution rate. High salicylate hydroxylase enzyme activity [236 units (U) l-1] and productivity (424.8 U h-1) were obtained at a dilution rate of 0.45 h-1 using a mineral medium containing 4 g l-1 of salicylate. Operating under continuous culture conditions with oxygen limitation and a slight accumulation of residual salicylate (0.2 g l-1) resulted in a decrease in culture performance and enzyme productivity.
AB - A salicylate-hydroxylase-producing strain of Pseudomonas putida with an unusual capability to grow at toxic levels of salicylate up to 10 g l-1 has been isolated. It grew well under continuous culture conditions, with optimum growth at pH 6.5 and a temperature of 25-degrees-C. The use of an ammonium salt as a nitrogen source, instead of nitrate, resulted in a 30-40% increase in its biomass yield coefficient. Optimum growth under continuous culture conditions was achieved using 4 g l-1 salicylate at 25-degrees-C, pH 6.5 and 0.2 h-1 dilution rate. High salicylate hydroxylase enzyme activity [236 units (U) l-1] and productivity (424.8 U h-1) were obtained at a dilution rate of 0.45 h-1 using a mineral medium containing 4 g l-1 of salicylate. Operating under continuous culture conditions with oxygen limitation and a slight accumulation of residual salicylate (0.2 g l-1) resulted in a decrease in culture performance and enzyme productivity.
M3 - Article
VL - 37
SP - 378
EP - 381
JO - Applied Microbiology and Biotechnology
T2 - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 3
ER -