The isolation and characterisation of a salicylate-hydroxylase-producing strain of Pseudomonas putida

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    Abstract

    A salicylate-hydroxylase-producing strain of Pseudomonas putida with an unusual capability to grow at toxic levels of salicylate up to 10 g l-1 has been isolated. It grew well under continuous culture conditions, with optimum growth at pH 6.5 and a temperature of 25° C. The use of an ammonium salt as a nitrogen source, instead of nitrate, resulted in a 30-40% increase in its biomass yield coefficient. Optimum growth under continuous culture conditions was achieved using 4 g l-1 salicylate at 25° C, pH 6.5 and 0.2 h-1 dilution rate. High salicylate hydroxylase enzyme activity [236 units (U) l-1] and productivity (424.8 U h-1) were obtained at a dilution rate of 0.45 h-1 using a mineral medium containing 4 g l-1 of salicylate. Operating under continuous culture conditions with oxygen limitation and a slight accumulation of residual salicylate (0.2 g l-1) resulted in a decrease in culture performance and enzyme productivity.

    Original languageEnglish
    Pages (from-to)378-381
    Number of pages4
    JournalApplied Microbiology and Biotechnology
    Volume37
    Issue number3
    DOIs
    Publication statusPublished - 1 Jun 1992

    Keywords

    • Biomass
    • Salicylate
    • Enzyme Productivity
    • Optimum Growth
    • Biomass Yield

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