The human neuroprotective placental protein composition suppressing tinnitus and restoring auditory brainstem response in a rodent model of sodium salicylate-induced ototoxicity

Mohammad Farhadi; Ali Gorji; Marjan Mirsalehi; Marcus Müller; Alexander Borisovich Poletaev; Fereidoun Mahboudi; Abdoreza Asadpour; Mohammad Ebrahimi; Mohaddeseh Beiranvand; Mohaddeseh Dehghani Khaftari; Zeinab Akbarnejad; Saeid Mahmoudian

doi:10.1016/j.heliyon.2023.e19052

The human neuroprotective placental protein composition suppressing tinnitus and restoring auditory brainstem response in a rodent model of sodium salicylate-induced ototoxicity

Mohammad Farhadi
, Ali Gorji
, Marjan Mirsalehi
, Marcus Müller
, Alexander Borisovich Poletaev
, Fereidoun Mahboudi
, Abdoreza Asadpour
, Mohammad Ebrahimi
, Mohaddeseh Beiranvand
, Mohaddeseh Dehghani Khaftari
, Zeinab Akbarnejad
, Saeid Mahmoudian

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

104 Downloads (Pure)

Abstract

The effect of neuroprotective placental protein composition (NPPC) on the suppression of tinnitus and the restoration of the auditory brainstem response (ABR) characteristics was explored in tinnitus-induced rats. The animals were placed into two groups: (1) the study group, rats received sodium salicylate (SS) at the dose of 200 mg/kg twice a day for two weeks, and then 0.4 mg of the NPPC per day, between the 14th and 28th days, (2) the placebo group, rats received saline for two weeks, and then the NPPC alone between the 14th and 28th days. The gap pre-pulse inhibition of the acoustic startle (GPIAS), the pre-pulse inhibition (PPI), and the ABR assessments were performed on animals in both groups three times (baseline, day 14, and 28). The GPIAS value declined after 14 consecutive days of the SS injection, while NPPC treatment augmented the GPIAS score in the study group on the 28th day. The PPI outcomes revealed no significant changes, indicating hearing preservation after the SS and NPPC administrations. Moreover, some changes in ABR characteristics were observed following SS injection, including (1) higher ABR thresholds, (2) lowered waves I and II amplitudes at the frequencies of 6, 12, and 24 kHz and wave III at the 12 kHz, (3) elevated amplitude ratios, and (4) prolongation in brainstem transmission time (BTT). All the mentioned variables returned to their normal values after applying the NPPC. The NPPC use could exert positive therapeutic effects on the tinnitus-induced rats and improve their ABR parameters.

Original language	English
Article number	e19052
Pages (from-to)	1-18
Number of pages	18
Journal	Heliyon
Volume	9
Issue number	8
Early online date	11 Aug 2023
DOIs	https://doi.org/10.1016/j.heliyon.2023.e19052
Publication status	Published (in print/issue) - 30 Aug 2023

Bibliographical note

Funding Information:
The support of the Iran National Science Foundation (INSF), ENT and Head & Neck Research Center, Iran University of Medical Sciences to assign credit to the research line auditory neuroscience is gratefully acknowledged. Also, all authors would like to appreciate the late Professor Alexander Borisovich Poletaev (*06 November 1951 – † March 6th, 2021). He was the first to report the evident positive effects of the protein fraction of porcine placental extract introduced by Alexander Anikin, in terms of having prominent neuroprotective properties, initially named X-proteins (XP). He also generously provided the placenta extract extraction method to this research group. Moreover, the authors would like to extend special thanks to Professor Marcus Müller (*06 November 1957 – †14 June 2020), Associate Professor of Physiology, Department of Otolaryngology, Head, and Neck Surgery of the University of Tübingen, Tübingen, Germany, for his unwavering support to set up the ABR system and record appropriate waveforms. He was a highly recognized auditory physiologist, tireless researcher, and dedicated teacher. The authors further thank all colleagues as well as administrative personnel at Shefa Neuroscience Institute, Tehran, Iran, who contributed to data collection.

Funding Information:
This study was ﬁnancially supported by the Iran National Science Foundation (INSF, Synergy Grant Code no. insf-98020383-1400/03/23), and ENT and Head & Neck Research Center, Hazrate Rasoul Akram Hospital, the Five Senses Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran (Ethical approval no. IR.IUMS.REC.1398.233).The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr. Mohammad Farhadi reports financial support was provided by Iran National Science Foundation. Dr. Saeid Mahmoudian reports financial support and administrative support were provided by ENT and Head and Neck Research Center, The Five Senses Health Institute, Iran University of Medical Sciences.

Funding Information:
This study was ﬁnancially supported by the Iran National Science Foundation (INSF, Synergy Grant Code no. insf-98020383-1400/03/23 ), and ENT and Head & Neck Research Center, Hazrate Rasoul Akram Hospital, the Five Senses Institute, School of Medicine, Iran University of Medical Sciences , Tehran, Iran (Ethical approval no. IR.IUMS.REC.1398.233 ).

Publisher Copyright:
© 2023

Data Availability Statement

Data included in article material/referenced in the article.

Funding

Funding Information: The support of the Iran National Science Foundation (INSF), ENT and Head & Neck Research Center, Iran University of Medical Sciences to assign credit to the research line auditory neuroscience is gratefully acknowledged. Also, all authors would like to appreciate the late Professor Alexander Borisovich Poletaev (*06 November 1951 – † March 6th, 2021). He was the first to report the evident positive effects of the protein fraction of porcine placental extract introduced by Alexander Anikin, in terms of having prominent neuroprotective properties, initially named X-proteins (XP). He also generously provided the placenta extract extraction method to this research group. Moreover, the authors would like to extend special thanks to Professor Marcus Müller (*06 November 1957 – †14 June 2020), Associate Professor of Physiology, Department of Otolaryngology, Head, and Neck Surgery of the University of Tübingen, Tübingen, Germany, for his unwavering support to set up the ABR system and record appropriate waveforms. He was a highly recognized auditory physiologist, tireless researcher, and dedicated teacher. The authors further thank all colleagues as well as administrative personnel at Shefa Neuroscience Institute, Tehran, Iran, who contributed to data collection. Funding Information: This study was ﬁnancially supported by the Iran National Science Foundation (INSF, Synergy Grant Code no. insf-98020383-1400/03/23), and ENT and Head & Neck Research Center, Hazrate Rasoul Akram Hospital, the Five Senses Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran (Ethical approval no. IR.IUMS.REC.1398.233).The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr. Mohammad Farhadi reports financial support was provided by Iran National Science Foundation. Dr. Saeid Mahmoudian reports financial support and administrative support were provided by ENT and Head and Neck Research Center, The Five Senses Health Institute, Iran University of Medical Sciences. Funding Information: This study was ﬁnancially supported by the Iran National Science Foundation (INSF, Synergy Grant Code no. insf-98020383-1400/03/23 ), and ENT and Head & Neck Research Center, Hazrate Rasoul Akram Hospital, the Five Senses Institute, School of Medicine, Iran University of Medical Sciences , Tehran, Iran (Ethical approval no. IR.IUMS.REC.1398.233 ). Publisher Copyright: © 2023

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Tinnitus
Evoked potentials
Auditory
Auditory brain stem response
Placental extract

Access to Document

10.1016/j.heliyon.2023.e19052

human neuroprotective placental protein composition suppressing tinnitusFinal published version, 6.2 MBLicence: CC BY-NC-ND

https://www.sciencedirect.com/science/article/pii/S2405844023062606

Cite this

Farhadi, M., Gorji, A., Mirsalehi, M., Müller, M., Poletaev, A. B., Mahboudi, F., Asadpour, A., Ebrahimi, M., Beiranvand, M., Khaftari, M. D., Akbarnejad, Z., & Mahmoudian, S. (2023). The human neuroprotective placental protein composition suppressing tinnitus and restoring auditory brainstem response in a rodent model of sodium salicylate-induced ototoxicity. Heliyon, 9(8), 1-18. Article e19052. https://doi.org/10.1016/j.heliyon.2023.e19052

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title = "The human neuroprotective placental protein composition suppressing tinnitus and restoring auditory brainstem response in a rodent model of sodium salicylate-induced ototoxicity",

abstract = "The effect of neuroprotective placental protein composition (NPPC) on the suppression of tinnitus and the restoration of the auditory brainstem response (ABR) characteristics was explored in tinnitus-induced rats. The animals were placed into two groups: (1) the study group, rats received sodium salicylate (SS) at the dose of 200 mg/kg twice a day for two weeks, and then 0.4 mg of the NPPC per day, between the 14th and 28th days, (2) the placebo group, rats received saline for two weeks, and then the NPPC alone between the 14th and 28th days. The gap pre-pulse inhibition of the acoustic startle (GPIAS), the pre-pulse inhibition (PPI), and the ABR assessments were performed on animals in both groups three times (baseline, day 14, and 28). The GPIAS value declined after 14 consecutive days of the SS injection, while NPPC treatment augmented the GPIAS score in the study group on the 28th day. The PPI outcomes revealed no significant changes, indicating hearing preservation after the SS and NPPC administrations. Moreover, some changes in ABR characteristics were observed following SS injection, including (1) higher ABR thresholds, (2) lowered waves I and II amplitudes at the frequencies of 6, 12, and 24 kHz and wave III at the 12 kHz, (3) elevated amplitude ratios, and (4) prolongation in brainstem transmission time (BTT). All the mentioned variables returned to their normal values after applying the NPPC. The NPPC use could exert positive therapeutic effects on the tinnitus-induced rats and improve their ABR parameters.",

keywords = "Tinnitus, Evoked potentials, Auditory, Auditory brain stem response, Placental extract",

author = "Mohammad Farhadi and Ali Gorji and Marjan Mirsalehi and Marcus M{\"u}ller and Poletaev, \{Alexander Borisovich\} and Fereidoun Mahboudi and Abdoreza Asadpour and Mohammad Ebrahimi and Mohaddeseh Beiranvand and Khaftari, \{Mohaddeseh Dehghani\} and Zeinab Akbarnejad and Saeid Mahmoudian",

note = "Funding Information: The support of the Iran National Science Foundation (INSF), ENT and Head \& Neck Research Center, Iran University of Medical Sciences to assign credit to the research line auditory neuroscience is gratefully acknowledged. Also, all authors would like to appreciate the late Professor Alexander Borisovich Poletaev (*06 November 1951 – † March 6th, 2021). He was the first to report the evident positive effects of the protein fraction of porcine placental extract introduced by Alexander Anikin, in terms of having prominent neuroprotective properties, initially named X-proteins (XP). He also generously provided the placenta extract extraction method to this research group. Moreover, the authors would like to extend special thanks to Professor Marcus M{\"u}ller (*06 November 1957 – †14 June 2020), Associate Professor of Physiology, Department of Otolaryngology, Head, and Neck Surgery of the University of T{\"u}bingen, T{\"u}bingen, Germany, for his unwavering support to set up the ABR system and record appropriate waveforms. He was a highly recognized auditory physiologist, tireless researcher, and dedicated teacher. The authors further thank all colleagues as well as administrative personnel at Shefa Neuroscience Institute, Tehran, Iran, who contributed to data collection. Funding Information: This study was ﬁnancially supported by the Iran National Science Foundation (INSF, Synergy Grant Code no. insf-98020383-1400/03/23), and ENT and Head \& Neck Research Center, Hazrate Rasoul Akram Hospital, the Five Senses Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran (Ethical approval no. IR.IUMS.REC.1398.233).The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr. Mohammad Farhadi reports financial support was provided by Iran National Science Foundation. Dr. Saeid Mahmoudian reports financial support and administrative support were provided by ENT and Head and Neck Research Center, The Five Senses Health Institute, Iran University of Medical Sciences. Funding Information: This study was ﬁnancially supported by the Iran National Science Foundation (INSF, Synergy Grant Code no. insf-98020383-1400/03/23 ), and ENT and Head \& Neck Research Center, Hazrate Rasoul Akram Hospital, the Five Senses Institute, School of Medicine, Iran University of Medical Sciences , Tehran, Iran (Ethical approval no. IR.IUMS.REC.1398.233 ). Publisher Copyright: {\textcopyright} 2023",

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Farhadi, M, Gorji, A, Mirsalehi, M, Müller, M, Poletaev, AB, Mahboudi, F, Asadpour, A, Ebrahimi, M, Beiranvand, M, Khaftari, MD, Akbarnejad, Z & Mahmoudian, S 2023, 'The human neuroprotective placental protein composition suppressing tinnitus and restoring auditory brainstem response in a rodent model of sodium salicylate-induced ototoxicity', Heliyon, vol. 9, no. 8, e19052, pp. 1-18. https://doi.org/10.1016/j.heliyon.2023.e19052

TY - JOUR

T1 - The human neuroprotective placental protein composition suppressing tinnitus and restoring auditory brainstem response in a rodent model of sodium salicylate-induced ototoxicity

AU - Farhadi, Mohammad

AU - Gorji, Ali

AU - Mirsalehi, Marjan

AU - Müller, Marcus

AU - Poletaev, Alexander Borisovich

AU - Mahboudi, Fereidoun

AU - Asadpour, Abdoreza

AU - Ebrahimi, Mohammad

AU - Beiranvand, Mohaddeseh

AU - Khaftari, Mohaddeseh Dehghani

AU - Akbarnejad, Zeinab

AU - Mahmoudian, Saeid

N1 - Funding Information: The support of the Iran National Science Foundation (INSF), ENT and Head & Neck Research Center, Iran University of Medical Sciences to assign credit to the research line auditory neuroscience is gratefully acknowledged. Also, all authors would like to appreciate the late Professor Alexander Borisovich Poletaev (*06 November 1951 – † March 6th, 2021). He was the first to report the evident positive effects of the protein fraction of porcine placental extract introduced by Alexander Anikin, in terms of having prominent neuroprotective properties, initially named X-proteins (XP). He also generously provided the placenta extract extraction method to this research group. Moreover, the authors would like to extend special thanks to Professor Marcus Müller (*06 November 1957 – †14 June 2020), Associate Professor of Physiology, Department of Otolaryngology, Head, and Neck Surgery of the University of Tübingen, Tübingen, Germany, for his unwavering support to set up the ABR system and record appropriate waveforms. He was a highly recognized auditory physiologist, tireless researcher, and dedicated teacher. The authors further thank all colleagues as well as administrative personnel at Shefa Neuroscience Institute, Tehran, Iran, who contributed to data collection. Funding Information: This study was ﬁnancially supported by the Iran National Science Foundation (INSF, Synergy Grant Code no. insf-98020383-1400/03/23), and ENT and Head & Neck Research Center, Hazrate Rasoul Akram Hospital, the Five Senses Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran (Ethical approval no. IR.IUMS.REC.1398.233).The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr. Mohammad Farhadi reports financial support was provided by Iran National Science Foundation. Dr. Saeid Mahmoudian reports financial support and administrative support were provided by ENT and Head and Neck Research Center, The Five Senses Health Institute, Iran University of Medical Sciences. Funding Information: This study was ﬁnancially supported by the Iran National Science Foundation (INSF, Synergy Grant Code no. insf-98020383-1400/03/23 ), and ENT and Head & Neck Research Center, Hazrate Rasoul Akram Hospital, the Five Senses Institute, School of Medicine, Iran University of Medical Sciences , Tehran, Iran (Ethical approval no. IR.IUMS.REC.1398.233 ). Publisher Copyright: © 2023

PY - 2023/8/30

Y1 - 2023/8/30

N2 - The effect of neuroprotective placental protein composition (NPPC) on the suppression of tinnitus and the restoration of the auditory brainstem response (ABR) characteristics was explored in tinnitus-induced rats. The animals were placed into two groups: (1) the study group, rats received sodium salicylate (SS) at the dose of 200 mg/kg twice a day for two weeks, and then 0.4 mg of the NPPC per day, between the 14th and 28th days, (2) the placebo group, rats received saline for two weeks, and then the NPPC alone between the 14th and 28th days. The gap pre-pulse inhibition of the acoustic startle (GPIAS), the pre-pulse inhibition (PPI), and the ABR assessments were performed on animals in both groups three times (baseline, day 14, and 28). The GPIAS value declined after 14 consecutive days of the SS injection, while NPPC treatment augmented the GPIAS score in the study group on the 28th day. The PPI outcomes revealed no significant changes, indicating hearing preservation after the SS and NPPC administrations. Moreover, some changes in ABR characteristics were observed following SS injection, including (1) higher ABR thresholds, (2) lowered waves I and II amplitudes at the frequencies of 6, 12, and 24 kHz and wave III at the 12 kHz, (3) elevated amplitude ratios, and (4) prolongation in brainstem transmission time (BTT). All the mentioned variables returned to their normal values after applying the NPPC. The NPPC use could exert positive therapeutic effects on the tinnitus-induced rats and improve their ABR parameters.

AB - The effect of neuroprotective placental protein composition (NPPC) on the suppression of tinnitus and the restoration of the auditory brainstem response (ABR) characteristics was explored in tinnitus-induced rats. The animals were placed into two groups: (1) the study group, rats received sodium salicylate (SS) at the dose of 200 mg/kg twice a day for two weeks, and then 0.4 mg of the NPPC per day, between the 14th and 28th days, (2) the placebo group, rats received saline for two weeks, and then the NPPC alone between the 14th and 28th days. The gap pre-pulse inhibition of the acoustic startle (GPIAS), the pre-pulse inhibition (PPI), and the ABR assessments were performed on animals in both groups three times (baseline, day 14, and 28). The GPIAS value declined after 14 consecutive days of the SS injection, while NPPC treatment augmented the GPIAS score in the study group on the 28th day. The PPI outcomes revealed no significant changes, indicating hearing preservation after the SS and NPPC administrations. Moreover, some changes in ABR characteristics were observed following SS injection, including (1) higher ABR thresholds, (2) lowered waves I and II amplitudes at the frequencies of 6, 12, and 24 kHz and wave III at the 12 kHz, (3) elevated amplitude ratios, and (4) prolongation in brainstem transmission time (BTT). All the mentioned variables returned to their normal values after applying the NPPC. The NPPC use could exert positive therapeutic effects on the tinnitus-induced rats and improve their ABR parameters.

KW - Tinnitus

KW - Evoked potentials

KW - Auditory

KW - Auditory brain stem response

KW - Placental extract

UR - https://doi.org/10.1016/j.heliyon.2023.e19052

UR - https://www.scopus.com/pages/publications/85168388675

U2 - 10.1016/j.heliyon.2023.e19052

DO - 10.1016/j.heliyon.2023.e19052

M3 - Article

C2 - 37636471

SN - 2405-8440

VL - 9

SP - 1

EP - 18

JO - Heliyon

JF - Heliyon

IS - 8

M1 - e19052

ER -

The human neuroprotective placental protein composition suppressing tinnitus and restoring auditory brainstem response in a rodent model of sodium salicylate-induced ototoxicity

Abstract

Bibliographical note

Data Availability Statement

Funding

UN SDGs

Keywords

Access to Document

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