The expression of proteinases and haemolysins by Aeromonas hydrophila under modified atmospheres

MAS McMahon

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    The study estimated the proteolytic activity (against Hide Powder Azure) and haemolytic activity (against horse erythrocytes) in cell-free filtrates (CFF) from four strains of Aeromonas hydrophila growing under a range of commercially relevant modified atmospheres (2% O-2, 78% N-2, 20% CO2; 10% O-2, 80%, N-2, 10% CO2; 50% N-2, 50% CO2; 100% CO2). The examined strains exhibited significant qualitative and quantitative differences in the extent and times of onset of expression of these enzymes under aerobic and modified atmospheres. No proteolytic or haemolytic activities were detected in any Aer. hydrophila cultures grown at sub-optimal temperatures under modified atmospheres containing high concentrations of CO2 (i.e. 50% CO2 or 100% CO2). Although Aer. hydrophila can grow rapidly in modified atmospheres, the overall spoilage and pathogenic potential is grossly affected. Implications of these findings are discussed.
    LanguageEnglish
    Pages415-422
    JournalJournal of Applied Microbiology
    Volume89
    Issue number3
    DOIs
    Publication statusPublished - Sep 2000

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    hemolysins
    Aeromonas hydrophila
    proteinases
    filtrates
    spoilage
    powders
    erythrocytes
    pathogenicity
    horses
    enzymes
    temperature
    cells

    Cite this

    @article{2c4972098b96403fac906bc09059c388,
    title = "The expression of proteinases and haemolysins by Aeromonas hydrophila under modified atmospheres",
    abstract = "The study estimated the proteolytic activity (against Hide Powder Azure) and haemolytic activity (against horse erythrocytes) in cell-free filtrates (CFF) from four strains of Aeromonas hydrophila growing under a range of commercially relevant modified atmospheres (2{\%} O-2, 78{\%} N-2, 20{\%} CO2; 10{\%} O-2, 80{\%}, N-2, 10{\%} CO2; 50{\%} N-2, 50{\%} CO2; 100{\%} CO2). The examined strains exhibited significant qualitative and quantitative differences in the extent and times of onset of expression of these enzymes under aerobic and modified atmospheres. No proteolytic or haemolytic activities were detected in any Aer. hydrophila cultures grown at sub-optimal temperatures under modified atmospheres containing high concentrations of CO2 (i.e. 50{\%} CO2 or 100{\%} CO2). Although Aer. hydrophila can grow rapidly in modified atmospheres, the overall spoilage and pathogenic potential is grossly affected. Implications of these findings are discussed.",
    author = "MAS McMahon",
    year = "2000",
    month = "9",
    doi = "10.1046/j.1365-2672.2000.01129x",
    language = "English",
    volume = "89",
    pages = "415--422",
    journal = "Journal of Applied Microbiology",
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    The expression of proteinases and haemolysins by Aeromonas hydrophila under modified atmospheres. / McMahon, MAS.

    In: Journal of Applied Microbiology, Vol. 89, No. 3, 09.2000, p. 415-422.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - The expression of proteinases and haemolysins by Aeromonas hydrophila under modified atmospheres

    AU - McMahon, MAS

    PY - 2000/9

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    AB - The study estimated the proteolytic activity (against Hide Powder Azure) and haemolytic activity (against horse erythrocytes) in cell-free filtrates (CFF) from four strains of Aeromonas hydrophila growing under a range of commercially relevant modified atmospheres (2% O-2, 78% N-2, 20% CO2; 10% O-2, 80%, N-2, 10% CO2; 50% N-2, 50% CO2; 100% CO2). The examined strains exhibited significant qualitative and quantitative differences in the extent and times of onset of expression of these enzymes under aerobic and modified atmospheres. No proteolytic or haemolytic activities were detected in any Aer. hydrophila cultures grown at sub-optimal temperatures under modified atmospheres containing high concentrations of CO2 (i.e. 50% CO2 or 100% CO2). Although Aer. hydrophila can grow rapidly in modified atmospheres, the overall spoilage and pathogenic potential is grossly affected. Implications of these findings are discussed.

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