The expression of membrane-associated 67-kDa laminin receptor (67LR) is modulated in vitro by cell-contact inhibition.

E A Donaldson, Declan McKenna, C B McMullen, W N Scott, A W Stitt, J Nelson

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The interaction of cells with their substratum is an important determinant of cell behaviour, influencing attachment, proliferation, and motility. Such interactions are mediated by cell surface receptors which bind to attachment factors, like the glycoprotein laminin in basement membranes. We have previously shown that expression of the 67-kDa laminin receptor (67LR) is elevated in proliferating retinal microvasculature compared with mature, quiescent vessels. Here, we examined 67LR mRNA and protein expression in primary cultures of retinal microvascular endothelial cells (RMEC) and in the breast cancer cell-line T47D during stages of contact inhibition. In both cell types, the expression levels of 67LR mRNA and membrane-associated 67LR protein were significantly increased during the proliferative phases of monolayer formation. As the cells achieved contact inhibition, 67LR expression was reduced to comparatively low levels. Thus, the differential expression of 67LR between dividing and contact-inhibited cells may indicate a role for this receptor during proliferative processes.
LanguageEnglish
Pages53-9
JournalMolecular cell biology research communications : MCBRC
Volume3
Issue number1
Publication statusPublished - 2000

Fingerprint

Laminin Receptors
Contact Inhibition
Membranes
Messenger RNA
Cell Surface Receptors
Laminin
Microvessels
Basement Membrane
Cell Communication
In Vitro Techniques
Glycoproteins
Proteins
Endothelial Cells
Breast Neoplasms
Cell Line

Keywords

  • retina
  • development
  • laminin receptor
  • microvasculature

Cite this

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title = "The expression of membrane-associated 67-kDa laminin receptor (67LR) is modulated in vitro by cell-contact inhibition.",
abstract = "The interaction of cells with their substratum is an important determinant of cell behaviour, influencing attachment, proliferation, and motility. Such interactions are mediated by cell surface receptors which bind to attachment factors, like the glycoprotein laminin in basement membranes. We have previously shown that expression of the 67-kDa laminin receptor (67LR) is elevated in proliferating retinal microvasculature compared with mature, quiescent vessels. Here, we examined 67LR mRNA and protein expression in primary cultures of retinal microvascular endothelial cells (RMEC) and in the breast cancer cell-line T47D during stages of contact inhibition. In both cell types, the expression levels of 67LR mRNA and membrane-associated 67LR protein were significantly increased during the proliferative phases of monolayer formation. As the cells achieved contact inhibition, 67LR expression was reduced to comparatively low levels. Thus, the differential expression of 67LR between dividing and contact-inhibited cells may indicate a role for this receptor during proliferative processes.",
keywords = "retina, development, laminin receptor, microvasculature",
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The expression of membrane-associated 67-kDa laminin receptor (67LR) is modulated in vitro by cell-contact inhibition. / Donaldson, E A; McKenna, Declan; McMullen, C B; Scott, W N; Stitt, A W; Nelson, J.

In: Molecular cell biology research communications : MCBRC, Vol. 3, No. 1, 2000, p. 53-9.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The expression of membrane-associated 67-kDa laminin receptor (67LR) is modulated in vitro by cell-contact inhibition.

AU - Donaldson, E A

AU - McKenna, Declan

AU - McMullen, C B

AU - Scott, W N

AU - Stitt, A W

AU - Nelson, J

PY - 2000

Y1 - 2000

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AB - The interaction of cells with their substratum is an important determinant of cell behaviour, influencing attachment, proliferation, and motility. Such interactions are mediated by cell surface receptors which bind to attachment factors, like the glycoprotein laminin in basement membranes. We have previously shown that expression of the 67-kDa laminin receptor (67LR) is elevated in proliferating retinal microvasculature compared with mature, quiescent vessels. Here, we examined 67LR mRNA and protein expression in primary cultures of retinal microvascular endothelial cells (RMEC) and in the breast cancer cell-line T47D during stages of contact inhibition. In both cell types, the expression levels of 67LR mRNA and membrane-associated 67LR protein were significantly increased during the proliferative phases of monolayer formation. As the cells achieved contact inhibition, 67LR expression was reduced to comparatively low levels. Thus, the differential expression of 67LR between dividing and contact-inhibited cells may indicate a role for this receptor during proliferative processes.

KW - retina

KW - development

KW - laminin receptor

KW - microvasculature

M3 - Article

VL - 3

SP - 53

EP - 59

JO - Molecular Cell Biology Research Communications

T2 - Molecular Cell Biology Research Communications

JF - Molecular Cell Biology Research Communications

SN - 1522-4724

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ER -