THE EFFECTS OF PHOSPHORIC-ACID PRETREATMENT ON CONVERSION OF CELLULOSE TO ETHANOL AT 45-DEGREES-C USING THE THERMOTOLERANT YEAST KLUYVEROMYCES-MARXIANUS IMB3

U NILSSON, N BARRON, L MCHALE, AP MCHALE

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Abstract

Here we report on the effects of phosphoric acid pretreated cellulose as a substrate for ethanol production by K. marxianus IMB3 using simultaneous saccharification and fermentation systems at 45 degrees C. With untreated, milled filter paper as substrate the maximum amount of ethanol produced was 25% of the maximum theoretical yield. After pre-treatment with 100% phosphoric acid, the yield increased to 42% of the maximum theoretical yield. When untreated microcrystalline cellulose was used as the fermentation substrate, yields of ethanol as 45 degrees C amounted to 16% of the maximum theoretical yield whereas pretreatment of the substrate with phosphoric acid resulted in an increase in ethanol production to 69% of the maximum theoretical yield. This suggests that pretreatment of substrate with phosphoric acid would contribute to a reduction in the amount of exogenous enzyme needed.
LanguageEnglish
Pages985-988
JournalBiotechnology Letters
Volume17
Issue number9
DOIs
Publication statusPublished - Sep 1995

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Kluyveromyces marxianus
phosphoric acid
cellulose
pretreatment
ethanol
yeasts
ethanol production
fermentation
saccharification
enzymes

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title = "THE EFFECTS OF PHOSPHORIC-ACID PRETREATMENT ON CONVERSION OF CELLULOSE TO ETHANOL AT 45-DEGREES-C USING THE THERMOTOLERANT YEAST KLUYVEROMYCES-MARXIANUS IMB3",
abstract = "Here we report on the effects of phosphoric acid pretreated cellulose as a substrate for ethanol production by K. marxianus IMB3 using simultaneous saccharification and fermentation systems at 45 degrees C. With untreated, milled filter paper as substrate the maximum amount of ethanol produced was 25{\%} of the maximum theoretical yield. After pre-treatment with 100{\%} phosphoric acid, the yield increased to 42{\%} of the maximum theoretical yield. When untreated microcrystalline cellulose was used as the fermentation substrate, yields of ethanol as 45 degrees C amounted to 16{\%} of the maximum theoretical yield whereas pretreatment of the substrate with phosphoric acid resulted in an increase in ethanol production to 69{\%} of the maximum theoretical yield. This suggests that pretreatment of substrate with phosphoric acid would contribute to a reduction in the amount of exogenous enzyme needed.",
author = "U NILSSON and N BARRON and L MCHALE and AP MCHALE",
year = "1995",
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THE EFFECTS OF PHOSPHORIC-ACID PRETREATMENT ON CONVERSION OF CELLULOSE TO ETHANOL AT 45-DEGREES-C USING THE THERMOTOLERANT YEAST KLUYVEROMYCES-MARXIANUS IMB3. / NILSSON, U; BARRON, N; MCHALE, L; MCHALE, AP.

In: Biotechnology Letters, Vol. 17, No. 9, 09.1995, p. 985-988.

Research output: Contribution to journalArticle

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AB - Here we report on the effects of phosphoric acid pretreated cellulose as a substrate for ethanol production by K. marxianus IMB3 using simultaneous saccharification and fermentation systems at 45 degrees C. With untreated, milled filter paper as substrate the maximum amount of ethanol produced was 25% of the maximum theoretical yield. After pre-treatment with 100% phosphoric acid, the yield increased to 42% of the maximum theoretical yield. When untreated microcrystalline cellulose was used as the fermentation substrate, yields of ethanol as 45 degrees C amounted to 16% of the maximum theoretical yield whereas pretreatment of the substrate with phosphoric acid resulted in an increase in ethanol production to 69% of the maximum theoretical yield. This suggests that pretreatment of substrate with phosphoric acid would contribute to a reduction in the amount of exogenous enzyme needed.

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