THE EFFECTS OF MN2+ ON ETHANOL-PRODUCTION BY KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA AT 45-DEGREES-C

D BRADY, R Marchant, L MCHALE, Anthony McHale

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Abstract

It has previously been demonstrated that the thermotolerant yeast strain, K. marxianus IMB3 is capable of growth and ethanol production on lactose containing media at 45 degrees C. Although the organism is capable of producing a beta-galactosidase, that enzyme has been shown to be extremely thermolabile at 45 degrees C and this has been reflected in reduced efficiencies with respect to conversion of lactose to ethanol. In this paper we demonstrate that addition of Mn2+ ions to enzyme preparations contributes significantly to enzyme stability at 45 degrees C. We also demonstrate that addition of Mn2+ to fermentations results in increased efficiency of conversion of lactose to ethanol at this temperature.
LanguageEnglish
Pages233-236
JournalBiotechnology Letters
Volume17
Issue number2
Publication statusPublished - Feb 1995

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Kluyveromyces marxianus
ethanol production
lactose
ethanol
enzyme stability
beta-galactosidase
enzymes
fermentation
ions
yeasts
organisms
temperature

Cite this

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title = "THE EFFECTS OF MN2+ ON ETHANOL-PRODUCTION BY KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA AT 45-DEGREES-C",
abstract = "It has previously been demonstrated that the thermotolerant yeast strain, K. marxianus IMB3 is capable of growth and ethanol production on lactose containing media at 45 degrees C. Although the organism is capable of producing a beta-galactosidase, that enzyme has been shown to be extremely thermolabile at 45 degrees C and this has been reflected in reduced efficiencies with respect to conversion of lactose to ethanol. In this paper we demonstrate that addition of Mn2+ ions to enzyme preparations contributes significantly to enzyme stability at 45 degrees C. We also demonstrate that addition of Mn2+ to fermentations results in increased efficiency of conversion of lactose to ethanol at this temperature.",
author = "D BRADY and R Marchant and L MCHALE and Anthony McHale",
year = "1995",
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journal = "Biotechnology Letters",
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AU - BRADY, D

AU - Marchant, R

AU - MCHALE, L

AU - McHale, Anthony

PY - 1995/2

Y1 - 1995/2

N2 - It has previously been demonstrated that the thermotolerant yeast strain, K. marxianus IMB3 is capable of growth and ethanol production on lactose containing media at 45 degrees C. Although the organism is capable of producing a beta-galactosidase, that enzyme has been shown to be extremely thermolabile at 45 degrees C and this has been reflected in reduced efficiencies with respect to conversion of lactose to ethanol. In this paper we demonstrate that addition of Mn2+ ions to enzyme preparations contributes significantly to enzyme stability at 45 degrees C. We also demonstrate that addition of Mn2+ to fermentations results in increased efficiency of conversion of lactose to ethanol at this temperature.

AB - It has previously been demonstrated that the thermotolerant yeast strain, K. marxianus IMB3 is capable of growth and ethanol production on lactose containing media at 45 degrees C. Although the organism is capable of producing a beta-galactosidase, that enzyme has been shown to be extremely thermolabile at 45 degrees C and this has been reflected in reduced efficiencies with respect to conversion of lactose to ethanol. In this paper we demonstrate that addition of Mn2+ ions to enzyme preparations contributes significantly to enzyme stability at 45 degrees C. We also demonstrate that addition of Mn2+ to fermentations results in increased efficiency of conversion of lactose to ethanol at this temperature.

M3 - Article

VL - 17

SP - 233

EP - 236

JO - Biotechnology Letters

T2 - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

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