The Effects of Environmental Stress on the Antibiotic Resistance Phenotype of Salmonella Typhimurium

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bacteria work to reduce the effects of sub-lethal stress by making phenotypic and genotypic changes. Phenotypic changes may provide resistance to further stress events and may also lead to cross-protection against a number of environmental challenges including resistance to antibiotics which bacteria see as a form of environmental stress. The effects of stress (temperature, pH, osmotic effects and starvation) on the antibiotic resistance phenotypes of S. Typhimurium DT104 were investigated in vitro. The antibiotics; tetracycline (T), streptomycin (S) and trimethoprim and sulfamethoxazole (SXT) were selected as resistance to T, S and SXT has been frequently observed in Salmonella isolated from farm animals in Northern Ireland. The investigation was carried out using standard antibiotic disk diffusion methodologies adapted to incorporate a stress inducing step. In terms of outcomes, starvation stress had the most effect on the susceptibility of Salmonella to the three antibiotics tested. Of the three antibiotics tested, Streptomycin was the least effective under starvation, osmotic and low temperature stresses. These results indicate that some types of sub-lethal stress may contribute to the expression of antibiotic resistance in Salmonella.
LanguageEnglish
Title of host publicationUnknown Host Publication
Pages78-79
Number of pages1
Publication statusAccepted/In press - 12 Jul 2016
Event5th ASM Conference on Salmonella - Potsdam, Germany
Duration: 12 Jul 2016 → …

Conference

Conference5th ASM Conference on Salmonella
Period12/07/16 → …

Fingerprint

Salmonella Typhimurium
antibiotic resistance
antibiotics
phenotype
starvation
Salmonella
streptomycin
sulfamethoxazole
Northern Ireland
trimethoprim
bacteria
farmed animal species
tetracycline
temperature
methodology

Keywords

  • Salmonella
  • Antibiotic Resistance

Cite this

@inproceedings{352efdc5ec43400e9e9311572b5932e4,
title = "The Effects of Environmental Stress on the Antibiotic Resistance Phenotype of Salmonella Typhimurium",
abstract = "Bacteria work to reduce the effects of sub-lethal stress by making phenotypic and genotypic changes. Phenotypic changes may provide resistance to further stress events and may also lead to cross-protection against a number of environmental challenges including resistance to antibiotics which bacteria see as a form of environmental stress. The effects of stress (temperature, pH, osmotic effects and starvation) on the antibiotic resistance phenotypes of S. Typhimurium DT104 were investigated in vitro. The antibiotics; tetracycline (T), streptomycin (S) and trimethoprim and sulfamethoxazole (SXT) were selected as resistance to T, S and SXT has been frequently observed in Salmonella isolated from farm animals in Northern Ireland. The investigation was carried out using standard antibiotic disk diffusion methodologies adapted to incorporate a stress inducing step. In terms of outcomes, starvation stress had the most effect on the susceptibility of Salmonella to the three antibiotics tested. Of the three antibiotics tested, Streptomycin was the least effective under starvation, osmotic and low temperature stresses. These results indicate that some types of sub-lethal stress may contribute to the expression of antibiotic resistance in Salmonella.",
keywords = "Salmonella, Antibiotic Resistance",
author = "Patrick Naughton and David Egan and Violetta Naughton and James Dooley",
year = "2016",
month = "7",
day = "12",
language = "English",
pages = "78--79",
booktitle = "Unknown Host Publication",

}

Naughton, P, Egan, D, Naughton, V & Dooley, J 2016, The Effects of Environmental Stress on the Antibiotic Resistance Phenotype of Salmonella Typhimurium. in Unknown Host Publication. pp. 78-79, 5th ASM Conference on Salmonella, 12/07/16.

The Effects of Environmental Stress on the Antibiotic Resistance Phenotype of Salmonella Typhimurium. / Naughton, Patrick; Egan, David; Naughton, Violetta; Dooley, James.

Unknown Host Publication. 2016. p. 78-79.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - The Effects of Environmental Stress on the Antibiotic Resistance Phenotype of Salmonella Typhimurium

AU - Naughton, Patrick

AU - Egan, David

AU - Naughton, Violetta

AU - Dooley, James

PY - 2016/7/12

Y1 - 2016/7/12

N2 - Bacteria work to reduce the effects of sub-lethal stress by making phenotypic and genotypic changes. Phenotypic changes may provide resistance to further stress events and may also lead to cross-protection against a number of environmental challenges including resistance to antibiotics which bacteria see as a form of environmental stress. The effects of stress (temperature, pH, osmotic effects and starvation) on the antibiotic resistance phenotypes of S. Typhimurium DT104 were investigated in vitro. The antibiotics; tetracycline (T), streptomycin (S) and trimethoprim and sulfamethoxazole (SXT) were selected as resistance to T, S and SXT has been frequently observed in Salmonella isolated from farm animals in Northern Ireland. The investigation was carried out using standard antibiotic disk diffusion methodologies adapted to incorporate a stress inducing step. In terms of outcomes, starvation stress had the most effect on the susceptibility of Salmonella to the three antibiotics tested. Of the three antibiotics tested, Streptomycin was the least effective under starvation, osmotic and low temperature stresses. These results indicate that some types of sub-lethal stress may contribute to the expression of antibiotic resistance in Salmonella.

AB - Bacteria work to reduce the effects of sub-lethal stress by making phenotypic and genotypic changes. Phenotypic changes may provide resistance to further stress events and may also lead to cross-protection against a number of environmental challenges including resistance to antibiotics which bacteria see as a form of environmental stress. The effects of stress (temperature, pH, osmotic effects and starvation) on the antibiotic resistance phenotypes of S. Typhimurium DT104 were investigated in vitro. The antibiotics; tetracycline (T), streptomycin (S) and trimethoprim and sulfamethoxazole (SXT) were selected as resistance to T, S and SXT has been frequently observed in Salmonella isolated from farm animals in Northern Ireland. The investigation was carried out using standard antibiotic disk diffusion methodologies adapted to incorporate a stress inducing step. In terms of outcomes, starvation stress had the most effect on the susceptibility of Salmonella to the three antibiotics tested. Of the three antibiotics tested, Streptomycin was the least effective under starvation, osmotic and low temperature stresses. These results indicate that some types of sub-lethal stress may contribute to the expression of antibiotic resistance in Salmonella.

KW - Salmonella

KW - Antibiotic Resistance

M3 - Conference contribution

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BT - Unknown Host Publication

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