The effects of copper deficiency on human lymphoid and myeloid cells: An in vitro model

KK Tong, BM Hannigan, George McKerr, JJ Strain

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Abstract

Cu has long been known to influence immune responses, An in vitro model system was established in which human myeloid (HL-60), B-Lymphoid (Raji) and T-lymphoid (Molt-3) cell Lines could be grown in culture media of varying Cu levels, initially Cu was removed from the medium by dialysis of fetal calf serum against a metal-ion chelator, minor depletion of other trace metals being obviated by repletion with appropriate metal salts, The growth rate of HL-60 was significantly (P < 0.05) inhibited by 72 h Cu depletion, Molt-3 cells required a longer period, up to 144 h, in Cu-depleted medium before growth was impaired, Raji-cell growth was not affected, These results confirmed clinical observations that T-cell functions were more sensitive to Cu deprivation than B cells, Analysis of intracellular metal levels in Molt-3 cells showed that Cu levels had been significantly lowered (P < 0.05) although Ca2+ levels were raised, Intracellular activity of the antioxidant enzyme superoxide dismutase (EC 1.15.1.1) was significantly impaired (P < 0.05) in Molt-3 cells grown in Cu-depleted medium, Activity of the mitochondrial enzyme cytochrome c oxidase (EC 1.9.3.1)was also significantly impaired (P < 0.05) by Cu depletion, Each of these findings indicates an increase in the potential for cellular damage by reduced antioxidant activity, impairment of normal mitochondrial activity and excessive Ca2+ influx, A major consequence of the type of damage occurring under these circumstances is membrane disruption, This was confirmed by scanning electron microscopy of Molt-3 cells groan under varying Cu levels.
LanguageEnglish
Pages97-108
JournalBRITISH JOURNAL OF NUTRITION
Volume75
Issue number1
Publication statusPublished - Jan 1996

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Myeloid Cells
Copper
Lymphocytes
Metals
Growth
Antioxidants
Electron Transport Complex IV
Enzymes
Chelating Agents
Electron Scanning Microscopy
Superoxide Dismutase
Culture Media
Dialysis
B-Lymphocytes
Salts
In Vitro Techniques
Ions
T-Lymphocytes
Cell Line
Membranes

Cite this

Tong, KK ; Hannigan, BM ; McKerr, George ; Strain, JJ. / The effects of copper deficiency on human lymphoid and myeloid cells: An in vitro model. In: BRITISH JOURNAL OF NUTRITION. 1996 ; Vol. 75, No. 1. pp. 97-108.
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abstract = "Cu has long been known to influence immune responses, An in vitro model system was established in which human myeloid (HL-60), B-Lymphoid (Raji) and T-lymphoid (Molt-3) cell Lines could be grown in culture media of varying Cu levels, initially Cu was removed from the medium by dialysis of fetal calf serum against a metal-ion chelator, minor depletion of other trace metals being obviated by repletion with appropriate metal salts, The growth rate of HL-60 was significantly (P < 0.05) inhibited by 72 h Cu depletion, Molt-3 cells required a longer period, up to 144 h, in Cu-depleted medium before growth was impaired, Raji-cell growth was not affected, These results confirmed clinical observations that T-cell functions were more sensitive to Cu deprivation than B cells, Analysis of intracellular metal levels in Molt-3 cells showed that Cu levels had been significantly lowered (P < 0.05) although Ca2+ levels were raised, Intracellular activity of the antioxidant enzyme superoxide dismutase (EC 1.15.1.1) was significantly impaired (P < 0.05) in Molt-3 cells grown in Cu-depleted medium, Activity of the mitochondrial enzyme cytochrome c oxidase (EC 1.9.3.1)was also significantly impaired (P < 0.05) by Cu depletion, Each of these findings indicates an increase in the potential for cellular damage by reduced antioxidant activity, impairment of normal mitochondrial activity and excessive Ca2+ influx, A major consequence of the type of damage occurring under these circumstances is membrane disruption, This was confirmed by scanning electron microscopy of Molt-3 cells groan under varying Cu levels.",
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The effects of copper deficiency on human lymphoid and myeloid cells: An in vitro model. / Tong, KK; Hannigan, BM; McKerr, George; Strain, JJ.

In: BRITISH JOURNAL OF NUTRITION, Vol. 75, No. 1, 01.1996, p. 97-108.

Research output: Contribution to journalArticle

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AB - Cu has long been known to influence immune responses, An in vitro model system was established in which human myeloid (HL-60), B-Lymphoid (Raji) and T-lymphoid (Molt-3) cell Lines could be grown in culture media of varying Cu levels, initially Cu was removed from the medium by dialysis of fetal calf serum against a metal-ion chelator, minor depletion of other trace metals being obviated by repletion with appropriate metal salts, The growth rate of HL-60 was significantly (P < 0.05) inhibited by 72 h Cu depletion, Molt-3 cells required a longer period, up to 144 h, in Cu-depleted medium before growth was impaired, Raji-cell growth was not affected, These results confirmed clinical observations that T-cell functions were more sensitive to Cu deprivation than B cells, Analysis of intracellular metal levels in Molt-3 cells showed that Cu levels had been significantly lowered (P < 0.05) although Ca2+ levels were raised, Intracellular activity of the antioxidant enzyme superoxide dismutase (EC 1.15.1.1) was significantly impaired (P < 0.05) in Molt-3 cells grown in Cu-depleted medium, Activity of the mitochondrial enzyme cytochrome c oxidase (EC 1.9.3.1)was also significantly impaired (P < 0.05) by Cu depletion, Each of these findings indicates an increase in the potential for cellular damage by reduced antioxidant activity, impairment of normal mitochondrial activity and excessive Ca2+ influx, A major consequence of the type of damage occurring under these circumstances is membrane disruption, This was confirmed by scanning electron microscopy of Molt-3 cells groan under varying Cu levels.

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