The effects of atomic force microscopy upon nominated living cells.

Barry O'Hagan, Peter Doyle, James M Allen, Kerry Sutton, G McKerr

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This work describes a system for precise re-location of cells within a monolayer after atomic force imaging. As we know little about probe interaction with soft biological surfaces any corroborative evidence is of great importance. For example, it is of paramount importance in living cell force microscopy that interrogated cells can be re-located and imaged by other corroborative technologies. Methodologies expressed here have shown that non-invasive force parameters can be established for specific cell types. Additionally, we show that the same sample can be transferred reliably to an SEM. Results here indicate that further work with live cells should initially establish appropriate prevailing force parameters and that cell damage should be checked for before and after an imaging experiment.
LanguageEnglish
Pages1-5
JournalUltramicroscopy
Volume102
Issue number1
Publication statusPublished - 2004

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Atomic force microscopy
Cells
atomic force microscopy
Imaging techniques
Relocation
cells
Monolayers
Microscopic examination
relocation
Scanning electron microscopy
Experiments
methodology
damage
microscopy
scanning electron microscopy
probes
interactions

Cite this

O'Hagan, B., Doyle, P., Allen, J. M., Sutton, K., & McKerr, G. (2004). The effects of atomic force microscopy upon nominated living cells. Ultramicroscopy, 102(1), 1-5.
O'Hagan, Barry ; Doyle, Peter ; Allen, James M ; Sutton, Kerry ; McKerr, G. / The effects of atomic force microscopy upon nominated living cells. In: Ultramicroscopy. 2004 ; Vol. 102, No. 1. pp. 1-5.
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O'Hagan, B, Doyle, P, Allen, JM, Sutton, K & McKerr, G 2004, 'The effects of atomic force microscopy upon nominated living cells.', Ultramicroscopy, vol. 102, no. 1, pp. 1-5.

The effects of atomic force microscopy upon nominated living cells. / O'Hagan, Barry; Doyle, Peter; Allen, James M; Sutton, Kerry; McKerr, G.

In: Ultramicroscopy, Vol. 102, No. 1, 2004, p. 1-5.

Research output: Contribution to journalArticle

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