The effect of oxygen transfer rate on continuous ethanol fermentation by Kluyveromyces marxianus

Catherine Hack, R Marchant

Research output: Contribution to journalArticle

Abstract

The effect of oxygen transfer rate on the growth rate, the specific ethanol productivity and the volumetric ethanol productivity of the thermotolerant yeast Kluyveromyces marxianus IMB3 in a continous ethanol fermentation was investigated. Under anaerobic conditions the strain grew poorly, with a maximum biomass concentration of 0.4 g L(-1). A small increase in oxygen transfer to the culture led to an increase in both the specific growth rate and the specific ethanol productivity. Further increases in the oxygen transfer rate to the culture caused a linear increase in the growth rate but a rapid decrease in the specific ethanol productivity. The specific ethanol productivity decreased to a constant minimum value of 0.25 (+/-0.05) g ethanol (g cells h)(-1). An initial maximum volumetric ethanol productivity, 0.14 g L(-1) h(-1) was achieved at an oxygen transfer rate to the culture broth of 3 mmol L(-1) h(-1). The volumetric ethanol productivity increased again as the biomass concentration continued to rise although the specific ethanol productivity had levelled, reaching a maximum of 0.44 g L(-1) h(-1). The biomass concentration in a non-aerated chemostat was estimated using the cell yield on oxygen and the oxygen transfer rate to the broth.
LanguageEnglish
Pages151-154
JournalPrehrambeno-tehnoloska i Biotehnoloska Revija
Volume33
Issue number4
Publication statusPublished - Oct 1995

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Kluyveromyces
Fermentation
Ethanol
Oxygen
Biomass
Growth
Yeasts

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abstract = "The effect of oxygen transfer rate on the growth rate, the specific ethanol productivity and the volumetric ethanol productivity of the thermotolerant yeast Kluyveromyces marxianus IMB3 in a continous ethanol fermentation was investigated. Under anaerobic conditions the strain grew poorly, with a maximum biomass concentration of 0.4 g L(-1). A small increase in oxygen transfer to the culture led to an increase in both the specific growth rate and the specific ethanol productivity. Further increases in the oxygen transfer rate to the culture caused a linear increase in the growth rate but a rapid decrease in the specific ethanol productivity. The specific ethanol productivity decreased to a constant minimum value of 0.25 (+/-0.05) g ethanol (g cells h)(-1). An initial maximum volumetric ethanol productivity, 0.14 g L(-1) h(-1) was achieved at an oxygen transfer rate to the culture broth of 3 mmol L(-1) h(-1). The volumetric ethanol productivity increased again as the biomass concentration continued to rise although the specific ethanol productivity had levelled, reaching a maximum of 0.44 g L(-1) h(-1). The biomass concentration in a non-aerated chemostat was estimated using the cell yield on oxygen and the oxygen transfer rate to the broth.",
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The effect of oxygen transfer rate on continuous ethanol fermentation by Kluyveromyces marxianus. / Hack, Catherine; Marchant, R.

Vol. 33, No. 4, 10.1995, p. 151-154.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of oxygen transfer rate on continuous ethanol fermentation by Kluyveromyces marxianus

AU - Hack, Catherine

AU - Marchant, R

PY - 1995/10

Y1 - 1995/10

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AB - The effect of oxygen transfer rate on the growth rate, the specific ethanol productivity and the volumetric ethanol productivity of the thermotolerant yeast Kluyveromyces marxianus IMB3 in a continous ethanol fermentation was investigated. Under anaerobic conditions the strain grew poorly, with a maximum biomass concentration of 0.4 g L(-1). A small increase in oxygen transfer to the culture led to an increase in both the specific growth rate and the specific ethanol productivity. Further increases in the oxygen transfer rate to the culture caused a linear increase in the growth rate but a rapid decrease in the specific ethanol productivity. The specific ethanol productivity decreased to a constant minimum value of 0.25 (+/-0.05) g ethanol (g cells h)(-1). An initial maximum volumetric ethanol productivity, 0.14 g L(-1) h(-1) was achieved at an oxygen transfer rate to the culture broth of 3 mmol L(-1) h(-1). The volumetric ethanol productivity increased again as the biomass concentration continued to rise although the specific ethanol productivity had levelled, reaching a maximum of 0.44 g L(-1) h(-1). The biomass concentration in a non-aerated chemostat was estimated using the cell yield on oxygen and the oxygen transfer rate to the broth.

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