The effect of cruciferous and leguminous sprouts on genotoxicity, in vitro and in vivo

Chris Gill, S Haldar, S Porter, S Matthews, S Sullivan, J Coulter, H McGlynn, I Rowland

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Vegetable consumption is associated with a reduced risk of colorectal cancer, which is the second most common cancer after lung/breast cancer within Europe. Some putative protective phytochemicals are found in higher amounts in young sprouts than in mature plants. The effect of an extract of mixed cruciferous and legume sprouts on DNA damage induced by H2O2 was measured in HT29 cells using single cell microgelelectrophoresis (comet). Significant antigenotoxic effect (P less than or equal to 0.05) was observed when HT29 cells were pre-incubated with the extract (100 and 200 muL/mL) for 24 hours and then challenged with H2O2. A parallel design intervention study was carried out on 10 male and 10 female healthy adult volunteers (mean age = 25.5 years) fed 113 g of cruciferous and legume sprouts daily for 14 days. The effect of the supplementation was measured on a range of parameters, including DNA damage in lymphocytes (comet), the activity of various detoxifying enzymes (glutathione S-transferase, glutathione peroxidase, and superoxide dismutase), antioxidant status using the ferric reducing ability of plasma assay, plasma antioxidants (uric acid, ascorbic acid, and alpha-tocopherol), blood lipids, plasma levels of lutein, and lycopene. A significant antigenotoxic effect against H2O2-induced DNA damage was shown in peripheral blood lymphocytes of volunteers who consumed the supplemented diet when compared with the control diet (P = 0.04). No significant induction of detoxifying enzymes was observed during the study, neither were plasma antioxidant levels or activity altered. The results support the theory that consumption of cruciferous vegetables is linked to a reduced risk of cancer via decreased damage to DNA.
LanguageEnglish
Pages1199+
JournalCANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
Volume13
Issue number7
Publication statusPublished - Jul 2004

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DNA Damage
HT29 Cells
Antioxidants
Fabaceae
Vegetables
Lung Neoplasms
Lymphocytes
Diet
Lutein
Enzyme Induction
Phytochemicals
alpha-Tocopherol
Glutathione Peroxidase
Uric Acid
Glutathione Transferase
Ascorbic Acid
Superoxide Dismutase
Volunteers
Colorectal Neoplasms
Healthy Volunteers

Cite this

Gill, C., Haldar, S., Porter, S., Matthews, S., Sullivan, S., Coulter, J., ... Rowland, I. (2004). The effect of cruciferous and leguminous sprouts on genotoxicity, in vitro and in vivo. CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, 13(7), 1199+.
Gill, Chris ; Haldar, S ; Porter, S ; Matthews, S ; Sullivan, S ; Coulter, J ; McGlynn, H ; Rowland, I. / The effect of cruciferous and leguminous sprouts on genotoxicity, in vitro and in vivo. In: CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION. 2004 ; Vol. 13, No. 7. pp. 1199+.
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Gill, C, Haldar, S, Porter, S, Matthews, S, Sullivan, S, Coulter, J, McGlynn, H & Rowland, I 2004, 'The effect of cruciferous and leguminous sprouts on genotoxicity, in vitro and in vivo', CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, vol. 13, no. 7, pp. 1199+.

The effect of cruciferous and leguminous sprouts on genotoxicity, in vitro and in vivo. / Gill, Chris; Haldar, S; Porter, S; Matthews, S; Sullivan, S; Coulter, J; McGlynn, H; Rowland, I.

In: CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, Vol. 13, No. 7, 07.2004, p. 1199+.

Research output: Contribution to journalArticle

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AU - Haldar, S

AU - Porter, S

AU - Matthews, S

AU - Sullivan, S

AU - Coulter, J

AU - McGlynn, H

AU - Rowland, I

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N2 - Vegetable consumption is associated with a reduced risk of colorectal cancer, which is the second most common cancer after lung/breast cancer within Europe. Some putative protective phytochemicals are found in higher amounts in young sprouts than in mature plants. The effect of an extract of mixed cruciferous and legume sprouts on DNA damage induced by H2O2 was measured in HT29 cells using single cell microgelelectrophoresis (comet). Significant antigenotoxic effect (P less than or equal to 0.05) was observed when HT29 cells were pre-incubated with the extract (100 and 200 muL/mL) for 24 hours and then challenged with H2O2. A parallel design intervention study was carried out on 10 male and 10 female healthy adult volunteers (mean age = 25.5 years) fed 113 g of cruciferous and legume sprouts daily for 14 days. The effect of the supplementation was measured on a range of parameters, including DNA damage in lymphocytes (comet), the activity of various detoxifying enzymes (glutathione S-transferase, glutathione peroxidase, and superoxide dismutase), antioxidant status using the ferric reducing ability of plasma assay, plasma antioxidants (uric acid, ascorbic acid, and alpha-tocopherol), blood lipids, plasma levels of lutein, and lycopene. A significant antigenotoxic effect against H2O2-induced DNA damage was shown in peripheral blood lymphocytes of volunteers who consumed the supplemented diet when compared with the control diet (P = 0.04). No significant induction of detoxifying enzymes was observed during the study, neither were plasma antioxidant levels or activity altered. The results support the theory that consumption of cruciferous vegetables is linked to a reduced risk of cancer via decreased damage to DNA.

AB - Vegetable consumption is associated with a reduced risk of colorectal cancer, which is the second most common cancer after lung/breast cancer within Europe. Some putative protective phytochemicals are found in higher amounts in young sprouts than in mature plants. The effect of an extract of mixed cruciferous and legume sprouts on DNA damage induced by H2O2 was measured in HT29 cells using single cell microgelelectrophoresis (comet). Significant antigenotoxic effect (P less than or equal to 0.05) was observed when HT29 cells were pre-incubated with the extract (100 and 200 muL/mL) for 24 hours and then challenged with H2O2. A parallel design intervention study was carried out on 10 male and 10 female healthy adult volunteers (mean age = 25.5 years) fed 113 g of cruciferous and legume sprouts daily for 14 days. The effect of the supplementation was measured on a range of parameters, including DNA damage in lymphocytes (comet), the activity of various detoxifying enzymes (glutathione S-transferase, glutathione peroxidase, and superoxide dismutase), antioxidant status using the ferric reducing ability of plasma assay, plasma antioxidants (uric acid, ascorbic acid, and alpha-tocopherol), blood lipids, plasma levels of lutein, and lycopene. A significant antigenotoxic effect against H2O2-induced DNA damage was shown in peripheral blood lymphocytes of volunteers who consumed the supplemented diet when compared with the control diet (P = 0.04). No significant induction of detoxifying enzymes was observed during the study, neither were plasma antioxidant levels or activity altered. The results support the theory that consumption of cruciferous vegetables is linked to a reduced risk of cancer via decreased damage to DNA.

M3 - Article

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SP - 1199+

JO - Cancer Epidemiology, Biomarkers and Prevention

T2 - Cancer Epidemiology, Biomarkers and Prevention

JF - Cancer Epidemiology, Biomarkers and Prevention

SN - 1055-9965

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