The Characterization of Salmonella isolated from Pig Meat in Northern Ireland by PFGE and Antibiotic Resistance Profiles

David Egan, Sarah Spence, J Dooley, Patrick Naughton

Research output: Contribution to conferenceAbstract

Abstract

The emergence of antibiotic resistance and especially multiple antibiotic resistance Salmonella has become a concern for the pig industry throughout the EU. Pig herds and pork are considered as principal reservoirs for the multi-resistant Salmonella type Typhimurium DT104, which has acquired resistance to ampicillin, chloramphenicol, streptomycin, sulphonamides and tetracycline. This resistance pattern is also known as ACSSuT. In this study Porcine Salmonella strains were isolated between December 2005 and December 2006. The strains originated from an abattoir study sampling the ‘oyster’ cut. The Antimicrobial Resistance Profiles of all Salmonella isolates in this study were determined by disk diffusion tests. Twelve antibiotics were utilized throughout the profiling procedure. Pulsed Field Gel Electrophoresis (PFGE), which is regarded as the “Gold Standard” for the typing and strain identification of Salmonella isolates, was used to determine DNA fingerprints of the Salmonella isolates using the restriction enzyme XbaI (Invitrogen). The fragments were then separated by PFGE in a Chef DR II system (Bio-Rad). This enabled comparison of Salmonella isolated in this study.
Original languageEnglish
Publication statusPublished - 9 May 2007
Event7th International Symposium on the epidemiology & control of foodborne pathogens in pork: SafePork 2007 - Palazzo della Gran Guardia, Verona, Italy
Duration: 9 May 200711 May 2007

Conference

Conference7th International Symposium on the epidemiology & control of foodborne pathogens in pork
CountryItaly
CityVerona
Period9/05/0711/05/07

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    Egan, D., Spence, S., Dooley, J., & Naughton, P. (2007). The Characterization of Salmonella isolated from Pig Meat in Northern Ireland by PFGE and Antibiotic Resistance Profiles. Abstract from 7th International Symposium on the epidemiology & control of foodborne pathogens in pork, Verona, Italy.